LOCUS       sequence-317509-        7655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast vector for expressing mCherry tagged genes under the GAL1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Roy B, Granas D, Bragg F Jr, Cher JAY, White MA, Stormo GD
  TITLE     Autoregulation of yeast ribosomal proteins discovered by efficient 
            search for feedback regulation.
  JOURNAL   Commun Biol. 2020 Dec 11;3(1):761. doi: 10.1038/s42003-020-01494-z.
  PUBMED    33311538
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-12-11"; volume: "3(1):761. doi"; pages: " 
            10.1038/s42003-020-01494-z"
COMMENT     Sequence Label: pMJB1
FEATURES             Location/Qualifiers
     source          1..7655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1451..1469)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1719..1738
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2197..2223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(2405..2423)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2444..2460)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2444..2460)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2457..2479)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2468..2484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2492..2498,2499..2516,2517..2522))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2537..2558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2675..2692)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2846..3434)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2926..2945)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3605..4396,4397..4465))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4228..4247
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4466..4570)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4607..5110
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5151..5169
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5207..5229)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5329..5348
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5372..5587
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5588..6391
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(6522..6977)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6659..6680)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6871..6890
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7107..7129
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7121..7138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7122..7138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7145..7164
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7145..7163
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        7181..7622
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    7181..7298
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     7593..7616
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 atgcttaagg gcccgcttaa gggctgcttg aatatgtcga agaaagtgat tgtaatagcc
       61 ggaactacag gtgttggcaa aagccaatta tctatacagt tggcacaaaa gtttaatggc
      121 gaagtaatta actctgattc catgcaagtt tataaagata taccgataat cactaataaa
      181 catccgctac aagagagaga agggattcct catcatgtca tgaatcacgt ggactggtca
      241 gaagagtact attcgcatag gttcgaaacg gaatgcatga acgctataga agacattcat
      301 agaaggggca aaatacctat tgttgttgga ggtacacact actatttaca gactctcttt
      361 aacaagcgag tagatactaa atcttccgaa cgtaaactaa ctcgtaaaca gttggatatt
      421 cttgaatcta cagatcctga tgtcatctac aatactttgg taaaatgtga ccccgacatt
      481 gcgacgaaat accatccgaa cgactatcgt agggtgcaaa gaatgcttga gatttattat
      541 aagacgggca aaaaaccatc tgaaacgttt aacgagcaaa agatcacttt gaaatttgac
      601 actcttttct tgtggctcta ttctaaaccc gaaccacttt ttcaaaggct ggatgacagg
      661 gtagatgata tgttagaaag aggggctttg caagaaataa aacaattgta cgaatactac
      721 agccaaaata aattcacacc tgaacaatgt gagaatggcg tttggcaggt aattggcttc
      781 aaggaatttc tgccttggct gacaggtaag acagatgata acactgtaaa actagaagat
      841 tgtatcgaga ggatgaaaac ccgaacccgt caatatgcta agcgtcaagt aaagtggatt
      901 aaaaaaatgt taataccgga tataaaaggt gatatctacc tattggatgc tacagatcta
      961 agtcaatggg acaccaatgc atcccaaagg gctatagcca ttagtaacga ctttatcagc
     1021 aatcggccta tcaagcaaga aagagctcct aaggcattgg aagaattact ttcaaagggt
     1081 gaaacaacta tgaaaaaatt ggatgattgg actcactata cgtgcaacgt atgcagaaat
     1141 gccgacggta aaaatgttgt tgcaattgga gaaaagtatt ggaaaataca tctaggttcc
     1201 cgtcggcata aatccaactt gaagagaaac acaagacagg cagactttga gaagtggaaa
     1261 attaataaaa aagagacggt ggaggtgagc aagggcgagg agcctagggt gagcaagggc
     1321 gaggaggata acatggccat catcaaggag ttcatgagat tcaaggtgca catggagggc
     1381 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggcagacc ctacgagggc
     1441 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1501 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1561 gactacttga agctgtcctt ccccgagggc ttcaagtggg agagagtgat gaacttcgag
     1621 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1681 aaggtgaagt tgagaggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1741 atgggctggg aggcctcctc cgagagaatg taccccgagg acggcgccct gaagggcgag
     1801 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     1861 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     1921 atcacctccc acaacgagga ctacaccatt gtggaacaat atgaaagagc tgaaggtaga
     1981 cattctactg gtggtatgga tgaattgtac aaataagcta gcatagatta atttaaacag
     2041 tatatgtaca gttttatata tatatatata tatatataca tatataaaga aacctgtgcg
     2101 ttttttgtat tttcaaatac atttagtttt ggcgccactt ctataaaagg tctcatgata
     2161 ttgttacaga tctgtttagc ttgcctcgag gtcgactatc catacgatgt tcctgattat
     2221 gcttagtgta catgacaccg attatttaaa gctgcagcat acgatatata tacatgtgta
     2281 tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata ctgaagatga
     2341 caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgcggat cccagctttt
     2401 gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
     2461 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2521 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2581 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2641 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2701 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2761 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2821 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2881 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2941 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3001 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3061 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3121 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3181 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3241 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3301 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3361 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3421 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3481 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3541 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3601 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3661 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3721 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3781 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3841 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3901 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3961 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4021 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4081 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4141 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4201 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4261 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4321 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4381 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4441 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4501 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4561 gggttccgcg cacatttccc cgaaaagtgc cacctgggtc cttttcatca cgtgctataa
     4621 aaataattat aatttaaatt ttttaatata aatatataaa ttaaaaatag aaagtaaaaa
     4681 aagaaattaa agaaaaaata gtttttgttt tccgaagatg taaaagactc tagggggatc
     4741 gccaacaaat actacctttt atcttgctct tcctgctctc aggtattaat gccgaattgt
     4801 ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg tgattttaca ttttacttat
     4861 cgttaatcga atgtatatct atttaatctg cttttcttgt ctaataaata tatatgtaaa
     4921 gtacgctttt tgttgaaatt ttttaaacct ttgtttattt ttttttcttc attccgtaac
     4981 tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa ataaataaac
     5041 acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg taagttacag
     5101 gcaagcgatc cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     5161 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     5221 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     5281 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     5341 agattgtact gagagtgcac cataccacct tttcaattca tcattttttt tttattcttt
     5401 tttttgattt cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga
     5461 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
     5521 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
     5581 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc
     5641 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt
     5701 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact
     5761 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa
     5821 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg
     5881 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat
     5941 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga
     6001 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc
     6061 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt
     6121 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt
     6181 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag
     6241 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt
     6301 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc
     6361 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat
     6421 actaaactca caaattagag cttcaattta attatatcag ttattaccct atgcggtgtg
     6481 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     6541 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     6601 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     6661 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     6721 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     6781 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     6841 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     6901 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     6961 gccgctacag ggcgcgtcgc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     7021 atcggtgcgg gcctcttcgc tattacgcca gctggcgaag gggggatgtg ctgcaaggcg
     7081 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     7141 attgtaatac gactcactat agggcgaatt ggcggccgca cggattagaa gccgccgagc
     7201 gggcgacagc cctccgacgg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt
     7261 tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac
     7321 tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa
     7381 ttaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta gccttatttc
     7441 tggggtaatt aatcagcgaa gcgatgattt ttgatctatt aacagatata taaatggaaa
     7501 agctgcataa ccactttaac taatactttc aacattttca gtttgtatta cttcttattc
     7561 aaatgtcata aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg
     7621 agaaggttaa ttaaggagaa aaaactatag ctagc
//
