LOCUS       sequence-31752-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Hui CC, Joyner AL
  TITLE     A mouse model of greig cephalopolysyndactyly syndrome: the 
            extra-toesJ mutation contains an intragenic deletion of the Gli3 
            gene.
  JOURNAL   Nat Genet. 1993 Mar . 3(3):241-6.
  PUBMED    8387379
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            1993 Mar . 3(3):241-6."
COMMENT     Sequence Label: pGli3a
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..19
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4..20
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(806..822)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(806..822)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(854..873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(855..873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(883..900)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(883..899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(892..914)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cctcgaggtc gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatcctc
       61 gccaaggcag ggggcagggt ggggcctgag agtggcttca agtcggcagg gttctgctgc
      121 ccctgatgct catgggtctg tcccggggaa agaggcaagc aggaggctga agtcatctac
      181 gagacaaact gccactggga aggctgcacc agagagttcg acacccaaga tcagcttgtg
      241 catcatatca ataatgacca cattcatgga gaaaagaagg agttcgtgtg ccgctggctt
      301 gattgttcac gagagcagaa accgttcaaa gcccagtaca tgttggtagt gcatatgaga
      361 agacacactg gggagaagcc tcacaaatgt acatttgaag gttgcacaaa agcctactca
      421 agactcgaaa acctgaaaac ccacttgaga tctcacactg gagagaagcc atacgtctgt
      481 gagcatgagg gctgcaacaa ggctttctct aatgcttcag atcgggccaa gcaccaaaac
      541 agaacacatt ccaatgagaa accgtatgta tgcaaaatcc ctggctgcac caagcgttac
      601 acagacccca gctctctccg gaaacacgtg aagactgtgc atggccctga ggctcatgtt
      661 accaagaagc agcgtgggga catgcaccct cggcctccac ccccgagaga ttccggcagc
      721 cattcacagt ccaggtcccc tggccggcca actcanggag catttgggga catgcaccct
      781 cggcctccac cccccatggt acccggatcc actagttcta gagcggccgc caccgcggtg
      841 gagctccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
      901 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      961 tttcgccagc tg
//
