LOCUS       sequence-317544-        8717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For stable integration of catalytic inactive FLAG-METTL3 in human 
            cells with trasposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Sorci M, Ianniello Z, Cruciani S, Larivera S, Ginistrelli LC, 
            Capuano E, Marchioni M, Fazi F, Fatica A
  TITLE     METTL3 regulates WTAP protein homeostasis.
  JOURNAL   Cell Death Dis. 2018 Jul 23;9(8):796. doi: 
            10.1038/s41419-018-0843-z.
  PUBMED    30038300
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2018-07-23"; volume: "9(8):796. doi"; pages: " 
            10.1038/s41419-018-0843-z"
COMMENT     Sequence Label: ePB-PURO-FLAG-METTL3-APPA
FEATURES             Location/Qualifiers
     source          1..8717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        84..398
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    92..110
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    128..146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    163..181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    199..217
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    235..253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    270..288
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    306..324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     371..395
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             439..462
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2324..2445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   2631..2693
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2728..2747)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2729..2747)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2757..2774)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2757..2773)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2766..2788)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2915..3370
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3002..3021)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3212..3233
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3396..3500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3501..3569,3570..4361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3719..3738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4532..5120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5021..5040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5274..5291
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5408..5429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5444..5449,5450..5467,5468..5474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5482..5498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5487..5509
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5506..5522
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5506..5522
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5541..5561
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5543..5561
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   5609..5643
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    5931..6052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6099..6698)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MAEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6182..6202)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(6702..6755)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(6756..6761,6762..6797,6798..6800,6801..6836,
                     6837..6839,6840..6875,6876..6881,6882..7499))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(7518..7565)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(7574..8717,1..68))
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(7718..7737)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 tgacgtggca gcgctcgccg tgaggagggg ggcgcccgcg gggggcgcca aaacccggcg
       61 cggaggccag atctcccgat ctcgagttta ctccctatca gtgatagaga acgtatgtcg
      121 agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag
      181 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
      241 tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag
      301 tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct
      361 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga gctcggtacc
      421 cggggatcca agcttatgga ttacaaggat gacgacgata agtcggacac gtggagctct
      481 atccaggccc acaagaagca gctggactct ctgcgggaga ggctgcagcg gaggcggaag
      541 caggactcgg ggcacttgga tctacggaat ccagaggcag cattgtctcc aaccttccgt
      601 agtgacagcc cagtgcctac tgcacccacc tctggtggcc ctaagcccag cacagcttca
      661 gcagttcctg aattagctac agatcctgag ttagagaaga agttgctaca ccacctctct
      721 gatctggcct taacattgcc cactgatgct gtgtccatct gtcttgccat ctccacgcca
      781 gatgctcctg ccactcaaga tggggtagaa agcctcctgc agaagtttgc agctcaggag
      841 ttgattgagg taaagcgagg tctcctacaa gatgatgcac atcctactct tgtaacctat
      901 gctgaccatt ccaagctctc tgccatgatg ggtgctgtgg cagaaaagaa gggccctggg
      961 gaggtagcag ggactgtcac agggcagaag cggcgtgcag aacaggactc gactacagta
     1021 gctgcctttg ccagttcgtt agtctctggt ctgaactctt cagcatcgga accagcaaag
     1081 gagccagcca agaaatcaag gaaacatgct gcctcagatg ttgatctgga gatagagagc
     1141 cttctgaacc aacagtccac taaggaacaa cagagcaaga aggtcagtca ggagatccta
     1201 gagctattaa atactacaac agccaaggaa caatccattg ttgaaaaatt tcgctctcga
     1261 ggtcgggccc aagtgcaaga attctgtgac tatggaacca aggaggagtg catgaaagcc
     1321 agtgatgctg atcgaccctg tcgcaagctg cacttcagac gaattatcaa taaacacact
     1381 gatgagtctt taggtgactg ctctttcctt aatacatgtt tccacatgga tacctgcaag
     1441 tatgttcact atgaaattga tgcttgcatg gattctgagg cccctggcag caaagaccac
     1501 acgccaagcc aggagcttgc tcttacacag agtgtcggag gtgattccag tgcagaccga
     1561 ctcttcccac ctcagtggat ctgttgtgat atccgctacc tggacgtcag tatcttgggc
     1621 aagtttgcag ttgtgatggc tgccccaccc gccgatattc acatggaact gccctatggg
     1681 accctgacag atgatgagat gcgcaggctc aacatacccg tactacagga tgatggcttt
     1741 ctcttcctct gggtcacagg cagggccatg gagttgggga gagaatgtct aaacctctgg
     1801 gggtatgaac gggtagatga aattatttgg gtgaagacaa atcaactgca acgcatcatt
     1861 cggacaggcc gtacaggtca ctggttgaac catgggaagg aacactgctt ggttggtgtc
     1921 aaaggaaatc cccaaggctt caaccagggt ctggattgtg atgtgatcgt agctgaggtt
     1981 cgttccacca gtcataaacc agatgaaatc tatggcatga ttgaaagact atctcctggc
     2041 actcgcaaga ttgagttatt tggacgacca cacaatgtgc aacccaactg gatcaccctt
     2101 ggaaaccaac tggatgggat ccacctacta gacccagatg tggttgcacg gttcaagcaa
     2161 aggtacccag atggtatcat ctctaaacct aagaatttat aggcggccgc gactctagat
     2221 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2281 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     2341 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2401 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggaa ttcgataaaa
     2461 gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
     2521 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
     2581 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
     2641 ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaatct
     2701 agctgcggcg cgccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     2761 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     2821 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2881 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     2941 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3001 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3061 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3121 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3181 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3241 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3301 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     3361 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3421 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3481 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3541 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3601 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3661 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3721 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3781 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3841 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3901 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3961 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4021 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4081 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4141 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4201 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4261 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4321 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4381 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4441 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4501 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4561 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4621 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4681 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4741 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4801 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4861 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4921 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4981 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5041 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5101 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5161 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5221 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5281 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5341 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     5401 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     5461 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     5521 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc
     5581 accgcggggc gcgccgcagc tagattaacc ctagaaagat agtctgcgta aaattgacgc
     5641 atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg ctgtgcattt
     5701 aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt
     5761 cactgatttt gaactataac aaccgcgtga gtcaaaatga cgcatgatta tcttttacgt
     5821 gacttttaag atttaactca tacgataatt atattgttat ttcgtgttct acttacgtga
     5881 taacttatta tatatatatt ttcttgttat agatgggaat tcagacatga taagatacat
     5941 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     6001 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag tttcgaggtc
     6061 gagtgtcagt cctgctcctc ggccacgaag tgcttagctc aggcaccggg cttgcgggtc
     6121 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     6181 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     6241 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     6301 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     6361 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     6421 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     6481 accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     6541 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccgggtcgac ggtgtggcgc
     6601 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggcccgggg gacgtcgtcg
     6661 cgggtggcga ggcgcaccgt gggcttgtac tcggccatgg tagggccggg attctcctcc
     6721 acgtcaccgc atgttagaag acttcctctg ccctccccgg ggagcatgtc aaggtcaaaa
     6781 tcgtcaagag cgtcagcagg cagcatatca aggtcaaagt cgtcaagggc atcggctggg
     6841 agcatgtcta agtcaaaatc gtcaagggcg tcggccggcc cgccgctttc gcactttagc
     6901 tgtttctcca ggccacatat gattagttcc aggccgaaaa ggaaggcagg ttcggctccc
     6961 tgccggtcga acagctcaat tgcttgtctc agaagtgggg gcatagaatc ggtggtaggt
     7021 gtctctcttt cctcttttgc tacttgatgc tcctgttcct ccaatacgca gcccagtgta
     7081 aagtggccca cggcggacag agcgtacagt gcgttctcca gggagaagcc ttgctgacac
     7141 aggaacgcga gctgattttc cagggtttcg tactgtttct ctgttgggcg ggtgccgaga
     7201 tgcactttag ccccgtcgcg atgtgagagg agagcacagc ggaatgactt ggcgttgttc
     7261 cgcagaaagt cttgccatga ctcgccttcc agggggcaga agtgggtatg atgcctgtcc
     7321 agcatctcga ttggcagggc atcgagcagg gcccgcttgt tcttcacgtg ccagtacagg
     7381 gtaggctgct caactcccag cttttgagcg agtttccttg tcgtcaggcc ttcgataccg
     7441 actccattga gtaattccag agcgccgttt atgactttgc tcttgtccag tctagacatg
     7501 gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga tgattaattg
     7561 tcaacacggt ccggtctaac aaaaaagcca aaaacggcca gaatttagcg gacaatttac
     7621 tagtctaaca ctgaaaatta catattgacc caaatgatta catttcaaaa ggtgcctaaa
     7681 aaacttcaca aaacacactc gccaaccccg agcgcatagt tcaaaaccgg agcttcagct
     7741 acttaagaag ataggtacat aaaaccgacc aaagaaactg acgcctcact tatccctccc
     7801 ctcaccagag gtccggcgcc tgtcgattca ggagagccta ccctaggccc gaaccctgcg
     7861 tcctgcgacg gagaaaagcc taccgcacac ctaccggcag gtggccccac cctgcattat
     7921 aagccaacag aacgggtgac gtcacgacac gacgagggcg cgcgctccca aaggtacggg
     7981 tgcactgccc aacggcaccg ccataactgc cgcccccgca acagacgaca aaccgagttc
     8041 tccagtcagt gacaaacttc acgtcagggt ccccagatgg tgccccagcc catctcaccc
     8101 gaataagagc tttcccgcat tagcgaaggc ctcaagacct tgggttcttg ccgcccacca
     8161 tgccccccac cttgtttcaa cgacctcaca gcccgcctca caagcgtctt ccattcaaga
     8221 ctcgggaaca gccgccattt tgctgcgctc cccccaaccc ccagttcagg gcaaccttgc
     8281 tcgcggaccc agactacagc ccttggcggt ctctccacac gcttccgtcc caccgagcgg
     8341 cccggcggcc acgaaagccc cggccagccc agcagcccgc tactcaccaa gtgacgatca
     8401 cagcgatcca caaacaagaa ccgcgaccca aatcccggct gcgacggaac tagctgtgcc
     8461 acacccggcg cgtccttata taatcatcgg cgttcaccgc cccacggaga tccctccgca
     8521 gaatcgccga gaagggacta cttttcctcg cctgttccgc tctctggaaa gaaaaccagt
     8581 gccctagagt cacccaagtc ccgtcctaaa atgtccttct gctgatactg gggttctaag
     8641 gccgagtctt atgagcagcg ggccgctgtc ctgagcgtcc gggcggaagg atcaggacgc
     8701 tcgctgcgcc cttcgtc
//
