LOCUS       sequence-31756-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     Drapkin Lab plasmids
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: piRES-puro-HE4
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          591..820
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     803..822
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agcttggtac cgagctcgga tcgatatctg cggcctagct agcgcttaag gcctgttaac
      121 cggtcgtacg tctccggatt cgaattcatg cctgcttgtc gcctaggccc gctagccgcc
      181 gccctcctcc tcagcctgct gctgttcggc ttcaccctag tctcaggcac aggagcagag
      241 aagactggcg tgtgccccga gctccaggct gaccagaact gcacgcaaga gtgcgtctcg
      301 gacagcgaat gcgccgacaa cctcaagtgc tgcagcgcgg gctgtgccac cttctgctct
      361 ctgcccaatg ataaggaggg ttcctgcccc caggtgaaca ttaactttcc ccagctcggc
      421 ctctgtcggg accagtgcca ggtggacagc cagtgtcctg gccagatgaa atgctgccgc
      481 aatggctgtg ggaaggtgtc ctgtgtcact cccaatttct gagcggccgc atagataact
      541 gatccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg gtgagtactc
      601 cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
      661 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga
      721 aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct ggccatacac
      781 ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
      841 ctgcaggtcg agcatgcatc tagg
//
