LOCUS       sequence-317665-        7133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase reporter containing the canonical Human IGF-1 P1 
            Promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Musicant AM, Parag-Sharma K, Gong W, Sengupta M, Chatterjee A, Henry
            EC, Tsai YH, Hayward MC, Sheth S, Betancourt R, Hackman TG, Padilla 
            RJ, Parker JS, Giudice J, Flaveny CA, Hayes DN, Amelio AL
  TITLE     CRTC1/MAML2 directs a PGC-1alpha-IGF-1 circuit that confers 
            vulnerability to PPARgamma inhibition.
  JOURNAL   Cell Rep. 2021 Feb 23;34(8):108768. doi: 
            10.1016/j.celrep.2021.108768.
  PUBMED    33626346
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-02-23"; volume: "34(8):108768. doi"; pages: " 
            10.1016/j.celrep.2021.108768"
COMMENT     Sequence Label: pIGF1_P1 Promoter-FLuc
FEATURES             Location/Qualifiers
     source          1..7133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..92
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     41..60
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      2250..2259
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2256..3908
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2322..2340)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3949..4070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3960..3979)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4014..4033
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4392..4412
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(4564..4581)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4735..5323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4815..4834)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5494..6285,6286..6354))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6117..6136
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6355..6459)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6486..6941
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6573..6592)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6783..6804
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    7072..7120
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
       61 cagtgcaagt gcaggtgcca gaacatttct ctatcgatag gtaccgagct cttacgcgtg
      121 ctagcccggg ctcgagatct ccaaagcctc tcatgacaca atctgcctcc ctcattttgg
      181 attttccaaa agattttact cccctgtctt cccagagaat gtgtcactcc ctctcattgt
      241 gcactgttca tgtttttaaa agagcacatg cccatcatat gactgtgaag ttttcctctt
      301 tgtctctgac attcagctga aagcccctga agggacttga ccatatctta ttcctctttg
      361 ttttcctagg gatgttgtag ttgggcacat agtagagctc acaaaatgtt gctgaacata
      421 gtgcaccatt gacacaacat aactccagat ttctaacttt gatttctgaa aggtaaacat
      481 tgctacaatg tattaaccac acagaagact caaacaaacc caaccccctt ccaaacaaag
      541 caggtttgtt tgttttttgt tagggcaccc acttctacaa acaaatttct ggccccagga
      601 taacacaaag agccagagta ggatttcaag cagaactgtg ttttcagttg atgtgtcagt
      661 cccctgagag tcatgtggaa aaaaaaaaaa aagaaaaaat tcaaggtcca ggttatttcc
      721 accactcctg ggaaaccagg cctggagagc tctctaggga aagaggtatg tctgctctgg
      781 gcttttgcaa ccttatttta taattcactt tcttatctac tgcttcacaa aaccaaaggg
      841 aaataggtac aaactgtatc gacaaaagat cagaactgaa ttctcaatgg caaaggcaag
      901 tgtacattat aaatagcaaa acagctggct tggaccatgt tgccggccag tcacccagtt
      961 gagggatttg aatgacatca taaccctcga gagggtattg ctagccagct ggtgttattt
     1021 agaatacaca aaaatcagag aaagaaaaca cactctggca cacagactcc ctctgtcata
     1081 cacacacaca cacacacaca cacacacaca cacacacaca ggtttgagtt atatggaaaa
     1141 ttcaaacaac aggaaaattg tttgcccccc aggtaccctt ctcccagagt ggtggggtgg
     1201 ggaggggaca gtgacaggca gcctagtaga agaataaaga aaaatgttct atttcagttg
     1261 ggttttacag ctcggcatag tctttgcctc atcgcaggag aaaaagtatg agacagtgcc
     1321 ctaaagggac caatccaatg ctgcctgccc ctccataggt tctaggaaat gagatcacac
     1381 ctctcacttg gcaactggga caaggggtca cccgagtgct gtcttccaat ctactttacc
     1441 ccagtcactt cagggttaaa attgtagagt ttgctggaga gggtcttatc gtcctttctt
     1501 tctttttttg ttttaaataa tgcatttgct ctagaatcta aaattgctct cccatccccc
     1561 atattccttt aatactggta aggtgtatta gcagacgttt gtgtcttcat gcccagcaga
     1621 aagttaatca gaaaacagat ccttattttc tatggcagca taagtatttt aatgtctgcg
     1681 aaccctgtca ctaacacaca ttcttttaag ggaaaaaaat gcttctgtgc tctagtttta
     1741 aaatgcaaag gtatgatgtt atttgtcacc atgcccaaaa aagtccttac tcaataactt
     1801 tgccagaaga gggagagaga gagaaggcaa atgttccccc agctgtttcc tgtctacagt
     1861 gtctgtgttt tgtagataaa tgtgaggatt ttctctaaat ccctcttctg tttgctaaat
     1921 ctcactgtca ctgctaaatt cagagcagat agagcctgcg caatggaata aagtcctcaa
     1981 aattgaaatg tgacattgct ctcaacatct cccatctctc tggatttctt tttgcttcat
     2041 tattcctgct aaccaattca ttttcagact ttgtacttca gaagcaatgg gaaaaatcag
     2101 cagtcttcca acccaattat ttaagtgctg cttttgtgat ttcttgaagg taaatatttc
     2161 ttactctttg aagtcattgg ggaattctat ttaaattgtg tactgtttgc ttctgcctag
     2221 aagcttggca ttccggtact gttggtaaag ccaccatgga agacgccaaa aacataaaga
     2281 aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg
     2341 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg
     2401 tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac
     2461 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct
     2521 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt
     2581 ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt
     2641 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa
     2701 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca
     2761 catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg
     2821 acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg
     2881 ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca
     2941 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa
     3001 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg
     3061 aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc
     3121 caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt
     3181 tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca
     3241 agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta
     3301 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt
     3361 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg
     3421 aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca
     3481 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag
     3541 acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg
     3601 tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg
     3661 tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc
     3721 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg
     3781 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa
     3841 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg
     3901 ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg
     3961 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4021 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4081 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     4141 aaaacctcta caaatgtggt aaaatcgata aggatctgaa cgatggagcg gagaatgggc
     4201 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     4261 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     4321 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     4381 ggactttcca caccctaact gacacacatt ccacagcgga tccgtcgacc gatgcccttg
     4441 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     4501 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     4561 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4621 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4681 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4741 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4801 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4861 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4921 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4981 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5041 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5101 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5161 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5221 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5281 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5341 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5401 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5461 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5521 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5581 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5641 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5701 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5761 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5821 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5881 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5941 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6001 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6061 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6121 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6181 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6241 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6301 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6361 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6421 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6481 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6541 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6601 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6661 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6721 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6781 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6841 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6901 atttaacgcg aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct
     6961 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct
     7021 accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata
     7081 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt act
//
