LOCUS       sequence-31767-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector with Tet-based inducible expression of mouse G 
            alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein 
            coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pSLIK-Venus-TmiR-G12
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           80..201
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           229..243
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           271..397
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(430..454)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatc tagtcgacta
       61 gggataacag ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac
      121 atcttggaaa cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagaag
      181 gtatattgct gttgacagtg agcgacgaca ccatcttcga caacattagt gaagccacag
      241 atgtaatgtt gtcgaagatg gtgtcgctgc ctactgcctc gcaattcaag gggctacttt
      301 aggagcaatt atcttgttta ctaaaactga ataccttgct atctctttga tacattttta
      361 caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattcgcgg ccgcactcga
      421 gatatctaga cccagctttc ttgtacaaag tggttgataa catctcgagc cataaagatg
      481 gttaattaac ccacccaaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
      541 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
      601 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
      661 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
      721 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
      781 tccgtggggc ggtgaacgcc gat
//
