LOCUS       sequence-317675-        3416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PKA in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Wallace AK, Chanut N, Voigt CA
  TITLE     Silica Nanostructures Produced Using Diatom Peptides with Designed 
            Post-Translational Modifications
  JOURNAL   Advanced Functional Materials 2020
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Advanced 
            Functional Materials 2020"
COMMENT     Sequence Label: pAKW.114A-PKA
FEATURES             Location/Qualifiers
     source          1..3416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1791..2336)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1871..1890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2425..3240)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2425..3240)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3148..3167
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3232..3251
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc
       61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac
      121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga
      181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctagac tgtcgccgga
      241 tgtgtatccg acctgacgat ggcccaaaag ggccgaaaca gtcctctaca aataattttg
      301 tttaacacaa caaaagccac taattattta tgggtaacgc tgccgccgcc aagaagggca
      361 gcgagcagga gagcgtgaaa gagttcctag ccaaagccaa ggaagatttc ctgaaaaaat
      421 gggaaacccc ttctcagaat acagcccagt tggatcagtt tgatagaatc aagacccttg
      481 gcaccggctc ctttgggcga gtgatgctgg tgaagcacaa ggagagtggg aaccactacg
      541 ccatgaagat cttagacaag cagaaggtgg tgaagctaaa gcagatcgag cacactctga
      601 atgagaagcg catcctgcag gccgtcaact tcccgttcct ggtcaaactt gaattctcct
      661 tcaaggacaa ctcaaacctg tacatggtca tggagtatgt agctggtggc gagatgttct
      721 cccacctacg gcggattgga aggttcagcg agccccatgc ccgtttctac gcggcgcaga
      781 tcgtcctgac ctttgagtat ctgcactccc tggacctcat ctaccgggac ctgaagcccg
      841 agaatcttct catcgaccag cagggctata ttcaggtgac agacttcggt tttgccaagc
      901 gtgtgaaagg ccgtacttgg accttgtgtg ggacccctga gtacttggcc cccgagatta
      961 tcctgagcaa aggctacaac aaggctgtgg actggtgggc tctcggagtc ctcatctacg
     1021 agatggctgc tggttaccca cccttcttcg ctgaccagcc tatccagatc tatgagaaaa
     1081 tcgtctctgg gaaggtgcgg ttcccatccc acttcagctc tgacttgaag gacctgctgc
     1141 ggaaccttct gcaagtggat ctcaccaagc gctttgggaa cctcaagaac ggggtcaatg
     1201 acatcaagaa ccacaagtgg tttgccacga ctgactggat tgccatctat cagagaaagg
     1261 tggaagctcc cttcatacca aagtttaaag gccctgggga cacgagtaac tttgacgact
     1321 atgaggagga agagatccgg gtttccatca atgagaagtg tggcaaggag tttactgagt
     1381 tttaataata ctagctcggt accaaattcc agaaaagaga cgctttcgag cgtctttttt
     1441 cgttttggta ccaagtgaga cccggcgcgc catcgaatga ggtgcttcct cgctcactga
     1501 ctcgctgcac gaggcagacc tcagcgctag cggagtgtat actggcttac tatgttggca
     1561 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
     1621 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
     1681 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     1741 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     1801 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     1861 acaggactat aaagatacca ggcgtttccc cctggtggct ccctcgtgcg ctctcctgtt
     1921 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     1981 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     2041 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     2101 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     2161 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     2221 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     2281 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     2341 atcatcttat taaggggtct gacgctcagt ggaacgaaaa atcaatctaa agtatatatg
     2401 agtaaacttg gtctgacagt taccttagaa aaactcatcg agcatcaaat gaaactgcaa
     2461 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     2521 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     2581 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     2641 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc
     2701 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     2761 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
     2821 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     2881 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     2941 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     3001 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattagcaac
     3061 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     3121 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     3181 atccatgttg gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa tatggctcat
     3241 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     3301 tttatcttgt gcaatgtaca tcagagattt tgagacacaa ccaattattg aaggcctccc
     3361 taacgggggg cctttttttg tttctggtct ccgtgctgct taacgatcgt tggctg
//
