LOCUS       sequence-317678-       10867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N. tabacum Rubisco large subunit and A. thaliana 
            Rubisco chaperone protiens cpn60-alpha, cpn60-beta, cpn20 in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Lin MT, Stone WD, Chaudhari V, Hanson MR
  TITLE     Small subunits can determine enzyme kinetics of tobacco Rubisco 
            expressed in Escherichia coli.
  JOURNAL   Nat Plants. 2020 Oct;6(10):1289-1299. doi: 
            10.1038/s41477-020-00761-5. Epub 2020 Sep 14.
  PUBMED    32929197
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-020-00761-5"; journalName: "Nat Plants"; date: 
            "2020-10"; volume: "6"; issue: "10"; pages: "1289-1299"
COMMENT     Sequence Label: pET-NtLAtC60-alpha-beta-C20
FEATURES             Location/Qualifiers
     source          1..10867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..61)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        129..233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(234..302,303..1094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(452..471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1265..1853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1754..1773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2007..2024
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2039..2178
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2104..2123)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2264..2286
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2280..2471)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3203..4081)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4108..4392
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4315..4334
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             4419..4441
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5905..5946
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5956..5973
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6029..6046)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6199..6285
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6377..6404
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     6457..6476
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6457..6475
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6476..6500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6515..6537
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             8186..8208
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             8626..8634
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             9901..9923
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(10615..10633)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10629..10676
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
       61 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      121 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      181 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      241 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      301 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      361 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      421 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
      481 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      541 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      601 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      661 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      721 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
      781 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      841 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      901 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      961 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1021 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1081 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1141 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1201 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1321 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1381 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1441 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1501 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1561 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1621 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1681 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1741 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1801 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1861 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1921 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     1981 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2041 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct
     2101 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     2161 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     2221 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     2281 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     2341 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     2401 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     2461 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     2521 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     2581 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     2641 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
     2701 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     2761 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     2821 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     2881 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc
     2941 caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
     3001 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
     3061 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcgaagcgg catgcataat
     3121 gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt
     3181 caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt
     3241 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
     3301 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
     3361 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
     3421 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
     3481 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
     3541 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
     3601 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
     3661 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
     3721 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
     3781 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
     3841 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
     3901 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
     3961 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
     4021 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
     4081 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt
     4141 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta
     4201 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
     4261 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
     4321 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
     4381 ctgtttctcc atacccgttt ttttgggcta gaaataattt tgtttaactt taagaaggag
     4441 atatacatac ccatgtcacc acaaacagag actaaagcaa gtgttggatt caaagctggt
     4501 gttaaagagt acaaattgac ttattatact cctgagtacc aaaccaagga tactgatata
     4561 ttggcagcat tccgagtaac tcctcaacct ggagttccac ctgaagaagc aggggccgcg
     4621 gtagctgccg aatcttctac tggtacatgg acaactgtat ggaccgatgg acttaccagc
     4681 cttgatcgtt acaaagggcg atgctaccgc atcgagcgtg ttgttggaga aaaagatcaa
     4741 tatattgctt atgtagctta ccctttagac ctttttgaag aaggttctgt taccaacatg
     4801 tttacttcca ttgtaggtaa cgtatttggg ttcaaagccc tgcgcgctct acgtctggaa
     4861 gatctgcgaa tccctcctgc ttatgttaaa actttccaag gtccgcctca tgggatccaa
     4921 gttgaaagag ataaattgaa caagtatggt cgtcccctgt tgggatgtac tattaaacct
     4981 aaattggggt tatctgctaa aaactacggt agagctgttt atgaatgtct tcgcggtgga
     5041 cttgatttta ccaaagatga tgagaacgtg aactcacaac catttatgcg ttggagagat
     5101 cgtttcttat tttgtgccga agcactttat aaagcacagg ctgaaacagg tgaaatcaaa
     5161 gggcattact tgaatgctac tgcaggtaca tgcgaagaaa tgatcaaaag agctgtattt
     5221 gctagagaat tgggcgttcc gatcgtaatg catgactact taacgggggg attcaccgca
     5281 aatactagct tggctcatta ttgccgagat aatggtctac ttcttcacat ccaccgtgca
     5341 atgcatgcgg ttattgatag acagaagaat catggtatcc acttccgggt attagcaaaa
     5401 gcgttacgta tgtctggtgg agatcatatt cactctggta ccgtagtagg taaacttgaa
     5461 ggtgaaagag acataacttt gggctttgtt gatttactgc gtgatgattt tgttgaacaa
     5521 gatcgaagtc gcggtattta tttcactcaa gattgggtct ctttaccagg tgttctaccc
     5581 gtggcttcag gaggtattca cgtttggcat atgcctgctc tgaccgagat ctttggggat
     5641 gattccgtac tacagttcgg tggaggaact ttaggacatc cttggggtaa tgcgccaggt
     5701 gccgtagcta atcgagtagc tctagaagca tgtgtaaaag ctcgtaatga aggacgtgat
     5761 cttgctcagg aaggtaatga aattattcgc gaggcttgca aatggagccc ggaactagct
     5821 gctgcttgtg aagtatggaa agagatcgta tttaattttg cagcagtgga cgttttggat
     5881 aagtaaacta gtactaagct tgaaggtaag cctatcccta accctctcct cggtctcgat
     5941 tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacggtctc cagcttggct
     6001 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
     6061 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
     6121 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
     6181 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     6241 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     6301 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     6361 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc ttttgccggc cacgatgcgt
     6421 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     6481 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
     6541 acatatgaat gtaaaggaaa tagcttttga ccagcactct agagctgctc tacaagctgg
     6601 tattgataag cttgctgatt gtgttggtct tacgcttggc cccagaggga ggaatgttgt
     6661 gttggatgaa tttggaagtc ctaaggttgt gaatgatgga gtcaccattg ctagagctat
     6721 tgagttacct aatgccatgg aaaatgctgg tgcagcgctt attcgtgagg ttgcgagtaa
     6781 gactaatgac tctgctggtg atgggacaac cactgcgtct atccttgctc gggagataat
     6841 caaacatgga ttgttgagcg tcacttctgg tgcgaatccc gtttcactca agaggggaat
     6901 tgataagact gttcaaggtc tgatcgaaga gcttcagaag aaagctaggc ccgtgaaagg
     6961 tcgtgatgac atcagagctg tggcttctat ctctgctgga aatgatgacc ttatagggtc
     7021 aatgattgct gatgccatcg acaaagttgg acctgatggt gttttgtcca ttgaatcttc
     7081 atcttctttt gagaccacgg tcgaagttga agaagggatg gagattgaca gaggttacat
     7141 ctcgcctcag tttgttacaa accctgagaa actactagct gagtttgaga atgctagggt
     7201 attgatcact gatcagaaga tcactgcgat caaagacatc atccctattt tggagaagac
     7261 cactcagctt cgtgctccgt tgttgattat tgcagaggat gttactggtg aagccttagc
     7321 tacccttgtc gtgaacaagc ttcgtggtgt cctcaatgtt gttgccgtta aagcgccagg
     7381 atttggagaa agaagaaaag ccatgcttca agatattgca atcttgacag gagctgagta
     7441 cctagccatg gacatgagct tactggttga aaacgcaacc atagatcaat tgggtattgc
     7501 ccgaaaagtc acgattagca aagattcgac taccctcatt gcagatgcag cttccaagga
     7561 cgaattacaa gctcggattg ctcagctgaa gaaagaacta tttgagactg attctgtgta
     7621 tgactcagag aagctcgctg agagaattgc gaagctatct ggaggtgttg ctgtcattaa
     7681 agtcggagca gcaactgaaa ctgagcttga ggaccgtaag cttcgtatcg aggatgcaaa
     7741 gaacgcaaca tttgctgcta tcgaggaagg catagttcca ggtggtggtg ctgctttggt
     7801 gcatctctcc actgttattc ccgccattaa ggagactttt gaggatgctg atgaacgttt
     7861 gggagctgac atagtacaaa aggcattgct gtcaccagct gctcttattg ctcagaacgc
     7921 tggagttgaa ggagaagttg ttgtggaaaa gattatgttc agcgattggg agaatgggta
     7981 caacgcaatg actgatactt acgagaatct gttcgaagct ggagtgattg atccggctaa
     8041 agtgacaaga tgtgcgctac agaacgctgc ttccgtagca ggaatggtac tgaccactca
     8101 ggccattgtt gttgacaaac cgaaacccaa ggctcctgct gctgctgctc ctgagggtct
     8161 catggtgtaa ggagctagaa ataattttgt ttaactttaa gaaggagata tacatatggc
     8221 agcaaaggaa ttacatttca acaaagatgg gactaccatt aggagacttc aagctggtgt
     8281 caacaagctc gcagatcttg ttggcgttac acttggaccc aaaggacgaa atgttgttct
     8341 tgagagcaag tatggatcac cacgaattgt taatgatggt gtgactgttg caagagaggt
     8401 tgagttggaa gacccggttg agaacattgg cgctaagctt gtgaggcaag cggctgccaa
     8461 aaccaatgac ttggctggtg acggtacaac aacatctgtt gttcttgcac aaggttttat
     8521 cgctgagggt gtcaaggtgg tagctgctgg tgcaaaccct gttttgatca ccagaggtat
     8581 tgagaagaca gcaaaggctt tggttaccga gctcaagaaa atgtctaagg aggttgaaga
     8641 cagtgaactt gcagatgttg cagctgttag tgcgggtaac aacgacgaaa ttggaaatat
     8701 gattgctgaa gcaatgagca aagtgggcag gaaaggtgtg gttacccttg aggagggtaa
     8761 aagtgccgag aacaacctct acgtcgtaga aggaatgcaa tttgaccgtg gttatatctc
     8821 tccttacttt gtgacagaca gcgagaaaat gtccgtcgag ttcgacaatt gcaagttact
     8881 tctcgttgac aagaaaatta ccaatgcaag ggatcttgtt ggtgttttgg aggatgcaat
     8941 tagaggcgga tacccaatat taattattgc tgaagacatt gaacaagaag ctttagcgac
     9001 tcttgttgta aacaagctta gaggtacact gaagatagca gctctcagag ctccagggtt
     9061 tggagagcgc aagagccaat accttgatga tattgccatt ctaactggag caactgtgat
     9121 ccgtgaggaa gttggtcttt cacttgacaa agctggaaaa gaggttcttg gtaatgcttc
     9181 aaaggttgtc ctcacgaagg agacctcgac catagtgggt gatggaagca cacaggatgc
     9241 agtgaaaaag cgtgttacac agattaaaaa tcttattgag caagcggagc aagattacga
     9301 gaaggagaaa ctcaatgaga gaattgcaaa gctctctggt ggagttgctg tgattcaggt
     9361 tggagcacaa actgagacag aactcaaaga gaagaagttg agagttgaag atgctcttaa
     9421 tgcaacaaag gccgctgtcg aggaaggtat tgttgttggt ggtggttgca ctctgcttcg
     9481 tcttgcttcc aaggttgatg ccattaaagc cacgcttgat aatgatgaag aaaaggttgg
     9541 agcggatata gttaaaagag cactaagtta ccccctaaaa ctaatcgcca agaatgctgg
     9601 tgttaatgga agtgtagtta gcgagaaggt gctttccaac gataacgtga aattcggtta
     9661 caatgctgca accggcaagt acgaggattt gatggctgca ggaatcatcg atccaacaaa
     9721 ggttgtgaga tgttgcttgg aacacgcagc ttcggttgca aagactttct tgatgtcgga
     9781 ctgcgttgtt gttgagatca aggaacctga accagttccc gtcggcaacc caatggacaa
     9841 ctcaggttac ggatactaag gtcgacgtcg accggataac aattgccctc tagaaataat
     9901 tttgtttaac tttaagaagg agatatacat atggcctctg ttgttgcccc taagtatact
     9961 tcaattaagc cattgggaga tcgagttttg gtgaagatca aggaggcaga ggagaagact
    10021 ttaggtggta tcttacttcc atccactgct caatcaaaac ctcaaggagg tgaagtcgtt
    10081 gctgtgggtg aaggaagaac tattgggaag aacaaaattg atatcactgt ccctactgga
    10141 gcacaaatta tctactccaa atacgcagga actgaggtgg agttcaatga tgtgaagcat
    10201 cttatcctca aggaagatga tattgttggc attcttgaga cagaggacat caaagatctc
    10261 aaacctttga atgaccgagt ctttattaag gttgctgagg cggaggagaa aacagctgga
    10321 gggttgttgt taaccgagac taccaaagag aagccttcta ttggcaccgt gatagcagtt
    10381 ggaccgggtt ccctagacga ggaaggtaaa attacgcctc taccagtatc aaccggaagc
    10441 acagtacttt actccaagta tgctggtaac gacttcaagg gcaaagatgg ttccaactac
    10501 attgccctca gagcttcaga tgtgatggct atactttctt agttatgtta tatctttgta
    10561 atctgcaaca agctgggtac cggtaccgaa aggaagctga gttggctgct gccaccgctg
    10621 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
    10681 aaggaggaac tatatccgga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
    10741 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
    10801 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagcta
    10861 gcttatc
//
