LOCUS       sequence-317680-       10976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco 
            chaperone protiens cpn60-alpha, cpn60-beta, cpn20 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   Lin MT, Stone WD, Chaudhari V, Hanson MR
  TITLE     Small subunits can determine enzyme kinetics of tobacco Rubisco 
            expressed in Escherichia coli.
  JOURNAL   Nat Plants. 2020 Oct;6(10):1289-1299. doi: 
            10.1038/s41477-020-00761-5. Epub 2020 Sep 14.
  PUBMED    32929197
REFERENCE   2  (bases 1 to 10976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-020-00761-5"; journalName: "Nat Plants"; date: 
            "2020-10"; volume: "6"; issue: "10"; pages: "1289-1299"
COMMENT     Sequence Label: pET-NtLNtC60-alpha-C20C60-beta
FEATURES             Location/Qualifiers
     source          1..10976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(31..49)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        117..221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(222..290,291..1082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(440..459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1253..1841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1742..1761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1995..2012
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2027..2166
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2092..2111)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2252..2274
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2268..2459)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3191..4069)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4096..4380
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4303..4322
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             4407..4429
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5893..5934
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5944..5961
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6017..6034)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6187..6273
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6365..6392
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6445..6463
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6464..6488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6503..6525
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7537..7548
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     RBS             8195..8217
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        8917..8935
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8936..8960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(10712..10730)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10726..10773
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
       61 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
      121 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      181 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      241 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      301 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
      361 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
      421 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
      481 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
      541 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
      601 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
      661 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
      721 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
      781 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
      841 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
      901 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
      961 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1021 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1081 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1141 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1201 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1261 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1321 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1381 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1441 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1501 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1561 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1621 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1681 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1741 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1801 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1861 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1921 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1981 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     2041 ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg
     2101 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     2161 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     2221 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     2281 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
     2341 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
     2401 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
     2461 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag
     2521 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
     2581 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
     2641 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg
     2701 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca
     2761 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg
     2821 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag
     2881 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg
     2941 ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc
     3001 caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga
     3061 gtggtgaatc cgttagcgag gtgccgccgg cgaagcggca tgcataatgt gcctgtcaaa
     3121 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
     3181 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
     3241 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
     3301 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
     3361 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
     3421 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
     3481 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
     3541 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
     3601 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
     3661 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
     3721 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
     3781 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
     3841 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
     3901 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
     3961 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
     4021 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
     4081 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
     4141 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     4201 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     4261 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     4321 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     4381 acccgttttt ttgggctaga aataattttg tttaacttta agaaggagat atacataccc
     4441 atgtcaccac aaacagagac taaagcaagt gttggattca aagctggtgt taaagagtac
     4501 aaattgactt attatactcc tgagtaccaa accaaggata ctgatatatt ggcagcattc
     4561 cgagtaactc ctcaacctgg agttccacct gaagaagcag gggccgcggt agctgccgaa
     4621 tcttctactg gtacatggac aactgtatgg accgatggac ttaccagcct tgatcgttac
     4681 aaagggcgat gctaccgcat cgagcgtgtt gttggagaaa aagatcaata tattgcttat
     4741 gtagcttacc ctttagacct ttttgaagaa ggttctgtta ccaacatgtt tacttccatt
     4801 gtaggtaacg tatttgggtt caaagccctg cgcgctctac gtctggaaga tctgcgaatc
     4861 cctcctgctt atgttaaaac tttccaaggt ccgcctcatg ggatccaagt tgaaagagat
     4921 aaattgaaca agtatggtcg tcccctgttg ggatgtacta ttaaacctaa attggggtta
     4981 tctgctaaaa actacggtag agctgtttat gaatgtcttc gcggtggact tgattttacc
     5041 aaagatgatg agaacgtgaa ctcacaacca tttatgcgtt ggagagatcg tttcttattt
     5101 tgtgccgaag cactttataa agcacaggct gaaacaggtg aaatcaaagg gcattacttg
     5161 aatgctactg caggtacatg cgaagaaatg atcaaaagag ctgtatttgc tagagaattg
     5221 ggcgttccga tcgtaatgca tgactactta acggggggat tcaccgcaaa tactagcttg
     5281 gctcattatt gccgagataa tggtctactt cttcacatcc accgtgcaat gcatgcggtt
     5341 attgatagac agaagaatca tggtatccac ttccgggtat tagcaaaagc gttacgtatg
     5401 tctggtggag atcatattca ctctggtacc gtagtaggta aacttgaagg tgaaagagac
     5461 ataactttgg gctttgttga tttactgcgt gatgattttg ttgaacaaga tcgaagtcgc
     5521 ggtatttatt tcactcaaga ttgggtctct ttaccaggtg ttctacccgt ggcttcagga
     5581 ggtattcacg tttggcatat gcctgctctg accgagatct ttggggatga ttccgtacta
     5641 cagttcggtg gaggaacttt aggacatcct tggggtaatg cgccaggtgc cgtagctaat
     5701 cgagtagctc tagaagcatg tgtaaaagct cgtaatgaag gacgtgatct tgctcaggaa
     5761 ggtaatgaaa ttattcgcga ggcttgcaaa tggagcccgg aactagctgc tgcttgtgaa
     5821 gtatggaaag agatcgtatt taattttgca gcagtggacg ttttggataa gtaaactagt
     5881 actaagcttg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     5941 ggtcatcatc accatcacca ttgagtttaa acggtctcca gcttggctgt tttggcggat
     6001 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     6061 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     6121 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     6181 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     6241 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     6301 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt
     6361 aagcagaagg ccatcctgac ggatggcctt ttgccggcca cgatgcgtcc ggcgtagagg
     6421 atcgagatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
     6481 acaattcccc tctagcaata attttgttta actttaagaa ggagatatac atatggctaa
     6541 agaaattgcc tttgaccaga aatctaggag tgcccttcag gctggtatcg ataagctcgc
     6601 tgatgctgtc ggtctcactc ttggtcccag ggggaggaat gtagtgttgg atgaatatgg
     6661 caccccaaag gtggttaatg acggagttac aattgctcgt gctatagagc tacctgatgc
     6721 tatggaaaat gctggcgctg cccttatcag ggaggttgca agcaaaacta acgattcagc
     6781 cggtgatgga accacgactg catctgttct tgctcgcgaa atcattaaac ttggtctatt
     6841 gagtgttaca tctggtgcaa atccagtgtc tttgaagaag ggcattgaca agactgtact
     6901 gggtttgatt gaagagctag aaaagaaggc tagacctgtt aaaggtcgtg atgacatcaa
     6961 agctattgct tcgatctctg ctggaaatga tgaaagcatt ggcaccatga ttgcggatgc
     7021 aattgacaaa gtcggtccag atggtgtgtt gtccatcgag tcatcctcct cctttgagac
     7081 aactgttcat gttgaagaag gaatggagat tgatagagga tatatttccc cacaatttgt
     7141 caccaaccct gagaaattaa ttgttgagtt tgagaatgct agagtcttag ttacggatca
     7201 gaagatctcg gctatcaagg atattatccc cctattggaa aagacaactc agttacgctc
     7261 tcctctgctc attattgctg aggatgtcac cggggaagct ctggctactc ttgtcgtgaa
     7321 caagctgcgg ggtatactga atgttgccgc catcaaagct cctggatttg gtgaaaggag
     7381 gaaggctctt ctgcaagata ttgccattgt gacaggggct gagtaccaag caactgacct
     7441 gggcctgctc attgagggta ccccagttga agcacttgga attgccagaa aggtgaccat
     7501 taccaaggat tcaacaacca tcattgctga tgcagcatcg aaggacgaga tacaggctag
     7561 gattgctcag ctcaaaaagg agttgtccga gacagactcg gtttatgact ccgagaaact
     7621 tgctgagaga atcgccaagt tgtctggggg tgttgccgtc atcaaggtcg gagctgctac
     7681 agaggctgag cttgaggacc gcaagcttcg catcgaggat gcaaagaatg caacttttgc
     7741 tgcaatcgaa gagggaatag tacctggtgg tggtgctgcc tttgttcatc tatcaactta
     7801 tgtccctgcc attaaggaca agctcgaaga tgcagatgaa aagttgggag ctgacatcat
     7861 tcaaagggca ttagtagcac cagcatcttt gatagcccaa aatgctggaa ttgaagggga
     7921 agtagtagtg gagaaggtaa gggaagctga atgggagatg ggttataacg cgatgacaga
     7981 caaatacgag aatcttgttg atgctggagt gatcgatcca gccaaggtga caagatgtgc
     8041 attgcagaat tcagcatcag ttgcaggaat ggtacttaca acacaagcca ttgtggtcga
     8101 gaaggcaaag cctaaggcag ctgcacctgc tgctccacaa gggctctcag tgtgatactt
     8161 ggcgcgcgga taacaattgc cctctagaaa taattttgtt taactttaag aaggagatat
     8221 acatatggct gctactgttg tagcccccaa atacacttct cttaagcctt tgggtgatag
     8281 agttttggtg aagattaaga ctgtagagga aaagaccgta ggtggtatcc tacttccaac
     8341 aacagcacag tcgaaacctc aaggaggtga ggttgttgct gttggggagg gtcgttcagt
     8401 tgggaagaat caggtggaca ttagtgtgaa gactggcacc caagttgttt actcaaaata
     8461 tgcgggaaca gaggtggagt tcaatggatc aaatcacctc atcttgaagg aggatgacat
     8521 tgttggtatt ctcgagacag atgaaatcaa ggatctgcag cccttgaatg acagagttct
     8581 gataaaggtc gccgaggctg aggaaaaaac tgctggaggc ttattattaa ctgaagcatc
     8641 caaggagaag ccttccattg gcacggttat agctgtaggt cctggtccac ttgacgagga
     8701 agggaaccgg aaaccactat cagtatccac aggaaataca gttctatact ccaaatatgc
     8761 tggcaatgaa ttcaaaggtg ctgatggttc tgagtacatt gcactgaggg catctgatgt
     8821 aatggctgtg ctttcatagg gcgcgcccag tgcggccgca taatgcttaa gtcgaacaga
     8881 aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact ataggggaat
     8941 tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa ggagatatac
     9001 atatacatat ggcagcaaag gagttgtatt tcaacaagga tggatcagct atcaagaggc
     9061 tgcaggttgg tgtgaacaaa ctcgctgatc tagttggagt tactcttggc ccaaaaggca
     9121 ggaatgttgt tcttgagagc aagtatggtg ctccaaagat tgtcaatgat ggagttactg
     9181 ttgccagaga ggttgagtta gaggatccag tagaaaacat tggtgctaaa ctagtgagac
     9241 aagctgctgc caaaaccaat gatttggctg gtgatgggac tacaacatct gtcgtactag
     9301 cccagggcct ggttgctgaa ggtgttaagg tggttgcagc tggtgcaaac cctgtcctga
     9361 tcaccagagg aattgagaag accacaaaag ccttagtagc tgaattgaag aatatgtcaa
     9421 aagaggtaga agacagtgaa ttagcagatg ttgctgcagt aagtgctggg aacaactctg
     9481 aagtagggag tatgattgct gaagccatga gcaaggttgg aaggaaaggt gttgtgactc
     9541 ttgaagaggg aaaaagtgct gaaaacagtc tccgtgtggt tgaaggaatg caatttgacc
     9601 gtggttacat ctcaccatac ttcgttactg atagtgagaa aatgtccgtt gagtatgaga
     9661 actgtaagtt attactggtt gacaagaaga taacaaatgc aagagatctt gttggtgtcc
     9721 tggaagatgc tatcagaaat ggttacccaa ttttaattat tgccgaagat attgagcaag
     9781 aagcactagc cacccttgtt gtcaataagc ttagaggtgc tttaaaggtt gctgccctta
     9841 aagctcctgg ttttggtgaa cgaaaaagcc agtatcttga tgacatagca acccttactg
     9901 gaggcactgt gattagggag gaacttggcc ttaccttaga caaggctgac aaggaagttc
     9961 taggccatgc ttctaaggta gtgctgacta aggatgccac cacaattgtt ggtgatggta
    10021 gcactcagga agcagtcaat aagcgtgttg tacagattaa aaacctcata gaggcggcag
    10081 atcaagatta tgaaaaagaa aagctaaatg aaagaattgc taaattatca ggaggtgtgg
    10141 ctgttataca ggttggagca caaactgaaa ctgaattgaa ggaaaagaag ctgagggtgg
    10201 aagatgctct caatgcaaca aaggcagctg ttgaggaagg tattgttgtt ggtggagggt
    10261 gcacactcct gcgacttgct tctaaagttg atgccatcaa ggagactctt gagaatgatg
    10321 aggagaaggt tggagctgat attgttaaaa gagcgttgag ctacccattg aagttgattg
    10381 ctaaaaatgc tggtgttaat ggaagtgttg tcagtgagaa ggtgctctct agtgacaatc
    10441 caaaatttgg atacaatgct gcaactggca attatgaaga cttgatggct gctggtatta
    10501 ttgacccaac aaaggtggtg agatgttgtc tagagcatgc gtcatcagtg gcgaagacat
    10561 tcttgatgtc agattgtgtg gtggtagaga tcaaggagcc tgaaccagca gttgctggca
    10621 acccaatgga caattcaggt tatggatact aattatgtta tatctttgta atctgcaacg
    10681 taccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
    10741 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat
    10801 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
    10861 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
    10921 agcaatttaa ctgtgataaa ctaccgcatt aaagctagct tatcgatgat aagctg
//
