LOCUS       sequence-317684-       10698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N. tabacum Rubisco large subunit (rbcL), Rubisco 
            chaperone protiens cpn60-alpha, cpn60-beta, and E. coli groES in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Lin MT, Stone WD, Chaudhari V, Hanson MR
  TITLE     Small subunits can determine enzyme kinetics of tobacco Rubisco 
            expressed in Escherichia coli.
  JOURNAL   Nat Plants. 2020 Oct;6(10):1289-1299. doi: 
            10.1038/s41477-020-00761-5. Epub 2020 Sep 14.
  PUBMED    32929197
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-020-00761-5"; journalName: "Nat Plants"; date: 
            "2020-10"; volume: "6"; issue: "10"; pages: "1289-1299"
COMMENT     Sequence Label: pET-NtLNtC60-alpha-ESC60-beta
FEATURES             Location/Qualifiers
     source          1..10698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..31)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        99..203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(204..272,273..1064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(422..441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1235..1823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1724..1743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1977..1994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2009..2148
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2074..2093)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2234..2256
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2250..2441)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3173..4051)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4078..4362
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4285..4304
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             4389..4411
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5875..5916
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5926..5943
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5999..6016)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6169..6255
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6347..6374
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6427..6445
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6446..6470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6485..6507
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7519..7530
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8165..8183
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             8215..8237
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8290..8343
                     /codon_start=1
                     /product="GroES chaperone mobile loop that interacts with 
                     GroEL"
                     /label=S loop
                     /translation="EVETKSAGGIVLTGSAAA"
     promoter        8621..8639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8640..8664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(10416..10434)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10430..10477
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
       61 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      181 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      241 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      301 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      361 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      421 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
      481 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      541 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
      601 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
      661 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
      721 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
      781 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
      841 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
      901 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
      961 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1021 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1381 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1921 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     1981 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     2041 gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2101 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     2161 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     2221 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     2281 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
     2341 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     2401 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     2461 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     2521 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     2581 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca
     2641 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg
     2701 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt
     2761 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt
     2821 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac
     2881 gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc
     2941 gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca
     3001 ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg
     3061 aggtgccgcc ggcgaagcgg catgcataat gtgcctgtca aatggacgaa gcagggattc
     3121 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca
     3181 acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg
     3241 ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc
     3301 gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac
     3361 gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca
     3421 gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg
     3481 ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga
     3541 gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca
     3601 gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc
     3661 tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg
     3721 gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc
     3781 attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa
     3841 tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa
     3901 tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat
     3961 aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg
     4021 gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag
     4081 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct
     4141 cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag
     4201 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt
     4261 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
     4321 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttgggcta
     4381 gaaataattt tgtttaactt taagaaggag atatacatac ccatgtcacc acaaacagag
     4441 actaaagcaa gtgttggatt caaagctggt gttaaagagt acaaattgac ttattatact
     4501 cctgagtacc aaaccaagga tactgatata ttggcagcat tccgagtaac tcctcaacct
     4561 ggagttccac ctgaagaagc aggggccgcg gtagctgccg aatcttctac tggtacatgg
     4621 acaactgtat ggaccgatgg acttaccagc cttgatcgtt acaaagggcg atgctaccgc
     4681 atcgagcgtg ttgttggaga aaaagatcaa tatattgctt atgtagctta ccctttagac
     4741 ctttttgaag aaggttctgt taccaacatg tttacttcca ttgtaggtaa cgtatttggg
     4801 ttcaaagccc tgcgcgctct acgtctggaa gatctgcgaa tccctcctgc ttatgttaaa
     4861 actttccaag gtccgcctca tgggatccaa gttgaaagag ataaattgaa caagtatggt
     4921 cgtcccctgt tgggatgtac tattaaacct aaattggggt tatctgctaa aaactacggt
     4981 agagctgttt atgaatgtct tcgcggtgga cttgatttta ccaaagatga tgagaacgtg
     5041 aactcacaac catttatgcg ttggagagat cgtttcttat tttgtgccga agcactttat
     5101 aaagcacagg ctgaaacagg tgaaatcaaa gggcattact tgaatgctac tgcaggtaca
     5161 tgcgaagaaa tgatcaaaag agctgtattt gctagagaat tgggcgttcc gatcgtaatg
     5221 catgactact taacgggggg attcaccgca aatactagct tggctcatta ttgccgagat
     5281 aatggtctac ttcttcacat ccaccgtgca atgcatgcgg ttattgatag acagaagaat
     5341 catggtatcc acttccgggt attagcaaaa gcgttacgta tgtctggtgg agatcatatt
     5401 cactctggta ccgtagtagg taaacttgaa ggtgaaagag acataacttt gggctttgtt
     5461 gatttactgc gtgatgattt tgttgaacaa gatcgaagtc gcggtattta tttcactcaa
     5521 gattgggtct ctttaccagg tgttctaccc gtggcttcag gaggtattca cgtttggcat
     5581 atgcctgctc tgaccgagat ctttggggat gattccgtac tacagttcgg tggaggaact
     5641 ttaggacatc cttggggtaa tgcgccaggt gccgtagcta atcgagtagc tctagaagca
     5701 tgtgtaaaag ctcgtaatga aggacgtgat cttgctcagg aaggtaatga aattattcgc
     5761 gaggcttgca aatggagccc ggaactagct gctgcttgtg aagtatggaa agagatcgta
     5821 tttaattttg cagcagtgga cgttttggat aagtaaacta gtactaagct tgaaggtaag
     5881 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
     5941 cattgagttt aaacggtctc cagcttggct gttttggcgg atgagagaag attttcagcc
     6001 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     6061 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     6121 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     6181 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     6241 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     6301 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     6361 acggatggcc ttttgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
     6421 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagcaa
     6481 taattttgtt taactttaag aaggagatat acatatggct aaagaaattg cctttgacca
     6541 gaaatctagg agtgcccttc aggctggtat cgataagctc gctgatgctg tcggtctcac
     6601 tcttggtccc agggggagga atgtagtgtt ggatgaatat ggcaccccaa aggtggttaa
     6661 tgacggagtt acaattgctc gtgctataga gctacctgat gctatggaaa atgctggcgc
     6721 tgcccttatc agggaggttg caagcaaaac taacgattca gccggtgatg gaaccacgac
     6781 tgcatctgtt cttgctcgcg aaatcattaa acttggtcta ttgagtgtta catctggtgc
     6841 aaatccagtg tctttgaaga agggcattga caagactgta ctgggtttga ttgaagagct
     6901 agaaaagaag gctagacctg ttaaaggtcg tgatgacatc aaagctattg cttcgatctc
     6961 tgctggaaat gatgaaagca ttggcaccat gattgcggat gcaattgaca aagtcggtcc
     7021 agatggtgtg ttgtccatcg agtcatcctc ctcctttgag acaactgttc atgttgaaga
     7081 aggaatggag attgatagag gatatatttc cccacaattt gtcaccaacc ctgagaaatt
     7141 aattgttgag tttgagaatg ctagagtctt agttacggat cagaagatct cggctatcaa
     7201 ggatattatc cccctattgg aaaagacaac tcagttacgc tctcctctgc tcattattgc
     7261 tgaggatgtc accggggaag ctctggctac tcttgtcgtg aacaagctgc ggggtatact
     7321 gaatgttgcc gccatcaaag ctcctggatt tggtgaaagg aggaaggctc ttctgcaaga
     7381 tattgccatt gtgacagggg ctgagtacca agcaactgac ctgggcctgc tcattgaggg
     7441 taccccagtt gaagcacttg gaattgccag aaaggtgacc attaccaagg attcaacaac
     7501 catcattgct gatgcagcat cgaaggacga gatacaggct aggattgctc agctcaaaaa
     7561 ggagttgtcc gagacagact cggtttatga ctccgagaaa cttgctgaga gaatcgccaa
     7621 gttgtctggg ggtgttgccg tcatcaaggt cggagctgct acagaggctg agcttgagga
     7681 ccgcaagctt cgcatcgagg atgcaaagaa tgcaactttt gctgcaatcg aagagggaat
     7741 agtacctggt ggtggtgctg cctttgttca tctatcaact tatgtccctg ccattaagga
     7801 caagctcgaa gatgcagatg aaaagttggg agctgacatc attcaaaggg cattagtagc
     7861 accagcatct ttgatagccc aaaatgctgg aattgaaggg gaagtagtag tggagaaggt
     7921 aagggaagct gaatgggaga tgggttataa cgcgatgaca gacaaatacg agaatcttgt
     7981 tgatgctgga gtgatcgatc cagccaaggt gacaagatgt gcattgcaga attcagcatc
     8041 agttgcagga atggtactta caacacaagc cattgtggtc gagaaggcaa agcctaaggc
     8101 agctgcacct gctgctccac aagggctctc agtgtgatac ttggcgcgcg gataacaatt
     8161 gccataatac gactcactat agggagacca caacggtttc cctctagaaa taattttgtt
     8221 taactttaag aaggagatat acatatgaat attcgtccat tgcatgatcg cgtgatcgtc
     8281 aagcgtaaag aagttgaaac taaatctgct ggcggcatcg ttctgaccgg ctctgcagcg
     8341 gctaaatcca cccgcggcga agtgctggct gtcggcaatg gccgtatcct tgaaaatggc
     8401 gaagtgaagc cgctggatgt gaaagttggc gacatcgtta ttttcaacga tggctacggt
     8461 gtgaaatctg agaagatcga caatgaagaa gtgttgatca tgtccgaaag cgacattctg
     8521 gcaattgttg aagcgtaata cttggcgcgc ccagtgcggc cgcataatgc ttaagtcgaa
     8581 cagaaagtaa tcgtattgta cacggccgca taatcgaaat taatacgact cactataggg
     8641 gaattgtgag cggataacaa ttccccatct tagtatatta gttaagtata agaaggagat
     8701 atacatatac atatggcagc aaaggagttg tatttcaaca aggatggatc agctatcaag
     8761 aggctgcagg ttggtgtgaa caaactcgct gatctagttg gagttactct tggcccaaaa
     8821 ggcaggaatg ttgttcttga gagcaagtat ggtgctccaa agattgtcaa tgatggagtt
     8881 actgttgcca gagaggttga gttagaggat ccagtagaaa acattggtgc taaactagtg
     8941 agacaagctg ctgccaaaac caatgatttg gctggtgatg ggactacaac atctgtcgta
     9001 ctagcccagg gcctggttgc tgaaggtgtt aaggtggttg cagctggtgc aaaccctgtc
     9061 ctgatcacca gaggaattga gaagaccaca aaagccttag tagctgaatt gaagaatatg
     9121 tcaaaagagg tagaagacag tgaattagca gatgttgctg cagtaagtgc tgggaacaac
     9181 tctgaagtag ggagtatgat tgctgaagcc atgagcaagg ttggaaggaa aggtgttgtg
     9241 actcttgaag agggaaaaag tgctgaaaac agtctccgtg tggttgaagg aatgcaattt
     9301 gaccgtggtt acatctcacc atacttcgtt actgatagtg agaaaatgtc cgttgagtat
     9361 gagaactgta agttattact ggttgacaag aagataacaa atgcaagaga tcttgttggt
     9421 gtcctggaag atgctatcag aaatggttac ccaattttaa ttattgccga agatattgag
     9481 caagaagcac tagccaccct tgttgtcaat aagcttagag gtgctttaaa ggttgctgcc
     9541 cttaaagctc ctggttttgg tgaacgaaaa agccagtatc ttgatgacat agcaaccctt
     9601 actggaggca ctgtgattag ggaggaactt ggccttacct tagacaaggc tgacaaggaa
     9661 gttctaggcc atgcttctaa ggtagtgctg actaaggatg ccaccacaat tgttggtgat
     9721 ggtagcactc aggaagcagt caataagcgt gttgtacaga ttaaaaacct catagaggcg
     9781 gcagatcaag attatgaaaa agaaaagcta aatgaaagaa ttgctaaatt atcaggaggt
     9841 gtggctgtta tacaggttgg agcacaaact gaaactgaat tgaaggaaaa gaagctgagg
     9901 gtggaagatg ctctcaatgc aacaaaggca gctgttgagg aaggtattgt tgttggtgga
     9961 gggtgcacac tcctgcgact tgcttctaaa gttgatgcca tcaaggagac tcttgagaat
    10021 gatgaggaga aggttggagc tgatattgtt aaaagagcgt tgagctaccc attgaagttg
    10081 attgctaaaa atgctggtgt taatggaagt gttgtcagtg agaaggtgct ctctagtgac
    10141 aatccaaaat ttggatacaa tgctgcaact ggcaattatg aagacttgat ggctgctggt
    10201 attattgacc caacaaaggt ggtgagatgt tgtctagagc atgcgtcatc agtggcgaag
    10261 acattcttga tgtcagattg tgtggtggta gagatcaagg agcctgaacc agcagttgct
    10321 ggcaacccaa tggacaattc aggttatgga tactaattat gttatatctt tgtaatctgc
    10381 aacgtaccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
    10441 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
    10501 atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg
    10561 gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg
    10621 cgttagcaat ttaactgtga taaactaccg cattaaagct agcttatcga tgataagctg
    10681 tcaaacatga gaattctt
//
