LOCUS       sequence-317688-       11227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N. tabacum Rubisco large subunit (rbcL) and A. 
            thaliana Rubisco chaperone protiens cpn60-alpha, cpn60-beta, cpn20 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Lin MT, Stone WD, Chaudhari V, Hanson MR
  TITLE     Small subunits can determine enzyme kinetics of tobacco Rubisco 
            expressed in Escherichia coli.
  JOURNAL   Nat Plants. 2020 Oct;6(10):1289-1299. doi: 
            10.1038/s41477-020-00761-5. Epub 2020 Sep 14.
  PUBMED    32929197
REFERENCE   2  (bases 1 to 11227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-020-00761-5"; journalName: "Nat Plants"; date: 
            "2020-10"; volume: "6"; issue: "10"; pages: "1289-1299"
COMMENT     Sequence Label: pET-AtC60-alpha-beta-C20NtL
FEATURES             Location/Qualifiers
     source          1..11227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..76)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        144..248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(249..317,318..1109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(467..486)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1280..1868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1769..1788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2022..2039
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2054..2193
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2119..2138)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2279..2301
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2295..2486)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3798..4880)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4842..4861
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4881..4958)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5164..5183
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5267..5285
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5286..5310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5325..5347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             6996..7018
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             7436..7444
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             8711..8733
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        9399..9417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9418..9442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             9457..9479
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(10990..11008)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11004..11051
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taaagctagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc
       61 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      181 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      241 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      301 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      361 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      421 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      481 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      541 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      601 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      661 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      721 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      781 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      841 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      901 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      961 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     1021 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     1081 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1141 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1201 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1261 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1321 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1381 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1441 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1501 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1561 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1621 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1681 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1741 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1801 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1861 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1921 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1981 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     2041 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2101 gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     2161 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     2221 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     2281 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg
     2341 taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc
     2401 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
     2461 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
     2521 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
     2581 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
     2641 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
     2701 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
     2761 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag
     2821 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
     2881 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
     2941 atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag
     3001 atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc
     3061 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt
     3121 ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa
     3181 ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat
     3241 aaatcgccgt gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg
     3301 atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg
     3361 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc
     3421 gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg
     3481 cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt
     3541 gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt
     3601 cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga
     3661 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg
     3721 ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta
     3781 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt
     3901 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
     3961 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
     4021 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata
     4081 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga
     4141 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt
     4201 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg
     4261 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg
     4321 cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc
     4381 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga
     4441 aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc
     4501 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta
     4561 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg
     4621 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg
     4681 gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg
     4741 taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg
     4801 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg
     4861 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat
     4921 gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt
     4981 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
     5041 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
     5101 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
     5161 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     5221 cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact
     5281 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
     5341 aaggagatat acatatgaat gtaaaggaaa tagcttttga ccagcactct agagctgctc
     5401 tacaagctgg tattgataag cttgctgatt gtgttggtct tacgcttggc cccagaggga
     5461 ggaatgttgt gttggatgaa tttggaagtc ctaaggttgt gaatgatgga gtcaccattg
     5521 ctagagctat tgagttacct aatgccatgg aaaatgctgg tgcagcgctt attcgtgagg
     5581 ttgcgagtaa gactaatgac tctgctggtg atgggacaac cactgcgtct atccttgctc
     5641 gggagataat caaacatgga ttgttgagcg tcacttctgg tgcgaatccc gtttcactca
     5701 agaggggaat tgataagact gttcaaggtc tgatcgaaga gcttcagaag aaagctaggc
     5761 ccgtgaaagg tcgtgatgac atcagagctg tggcttctat ctctgctgga aatgatgacc
     5821 ttatagggtc aatgattgct gatgccatcg acaaagttgg acctgatggt gttttgtcca
     5881 ttgaatcttc atcttctttt gagaccacgg tcgaagttga agaagggatg gagattgaca
     5941 gaggttacat ctcgcctcag tttgttacaa accctgagaa actactagct gagtttgaga
     6001 atgctagggt attgatcact gatcagaaga tcactgcgat caaagacatc atccctattt
     6061 tggagaagac cactcagctt cgtgctccgt tgttgattat tgcagaggat gttactggtg
     6121 aagccttagc tacccttgtc gtgaacaagc ttcgtggtgt cctcaatgtt gttgccgtta
     6181 aagcgccagg atttggagaa agaagaaaag ccatgcttca agatattgca atcttgacag
     6241 gagctgagta cctagccatg gacatgagct tactggttga aaacgcaacc atagatcaat
     6301 tgggtattgc ccgaaaagtc acgattagca aagattcgac taccctcatt gcagatgcag
     6361 cttccaagga cgaattacaa gctcggattg ctcagctgaa gaaagaacta tttgagactg
     6421 attctgtgta tgactcagag aagctcgctg agagaattgc gaagctatct ggaggtgttg
     6481 ctgtcattaa agtcggagca gcaactgaaa ctgagcttga ggaccgtaag cttcgtatcg
     6541 aggatgcaaa gaacgcaaca tttgctgcta tcgaggaagg catagttcca ggtggtggtg
     6601 ctgctttggt gcatctctcc actgttattc ccgccattaa ggagactttt gaggatgctg
     6661 atgaacgttt gggagctgac atagtacaaa aggcattgct gtcaccagct gctcttattg
     6721 ctcagaacgc tggagttgaa ggagaagttg ttgtggaaaa gattatgttc agcgattggg
     6781 agaatgggta caacgcaatg actgatactt acgagaatct gttcgaagct ggagtgattg
     6841 atccggctaa agtgacaaga tgtgcgctac agaacgctgc ttccgtagca ggaatggtac
     6901 tgaccactca ggccattgtt gttgacaaac cgaaacccaa ggctcctgct gctgctgctc
     6961 ctgagggtct catggtgtaa ggagctagaa ataattttgt ttaactttaa gaaggagata
     7021 tacatatggc agcaaaggaa ttacatttca acaaagatgg gactaccatt aggagacttc
     7081 aagctggtgt caacaagctc gcagatcttg ttggcgttac acttggaccc aaaggacgaa
     7141 atgttgttct tgagagcaag tatggatcac cacgaattgt taatgatggt gtgactgttg
     7201 caagagaggt tgagttggaa gacccggttg agaacattgg cgctaagctt gtgaggcaag
     7261 cggctgccaa aaccaatgac ttggctggtg acggtacaac aacatctgtt gttcttgcac
     7321 aaggttttat cgctgagggt gtcaaggtgg tagctgctgg tgcaaaccct gttttgatca
     7381 ccagaggtat tgagaagaca gcaaaggctt tggttaccga gctcaagaaa atgtctaagg
     7441 aggttgaaga cagtgaactt gcagatgttg cagctgttag tgcgggtaac aacgacgaaa
     7501 ttggaaatat gattgctgaa gcaatgagca aagtgggcag gaaaggtgtg gttacccttg
     7561 aggagggtaa aagtgccgag aacaacctct acgtcgtaga aggaatgcaa tttgaccgtg
     7621 gttatatctc tccttacttt gtgacagaca gcgagaaaat gtccgtcgag ttcgacaatt
     7681 gcaagttact tctcgttgac aagaaaatta ccaatgcaag ggatcttgtt ggtgttttgg
     7741 aggatgcaat tagaggcgga tacccaatat taattattgc tgaagacatt gaacaagaag
     7801 ctttagcgac tcttgttgta aacaagctta gaggtacact gaagatagca gctctcagag
     7861 ctccagggtt tggagagcgc aagagccaat accttgatga tattgccatt ctaactggag
     7921 caactgtgat ccgtgaggaa gttggtcttt cacttgacaa agctggaaaa gaggttcttg
     7981 gtaatgcttc aaaggttgtc ctcacgaagg agacctcgac catagtgggt gatggaagca
     8041 cacaggatgc agtgaaaaag cgtgttacac agattaaaaa tcttattgag caagcggagc
     8101 aagattacga gaaggagaaa ctcaatgaga gaattgcaaa gctctctggt ggagttgctg
     8161 tgattcaggt tggagcacaa actgagacag aactcaaaga gaagaagttg agagttgaag
     8221 atgctcttaa tgcaacaaag gccgctgtcg aggaaggtat tgttgttggt ggtggttgca
     8281 ctctgcttcg tcttgcttcc aaggttgatg ccattaaagc cacgcttgat aatgatgaag
     8341 aaaaggttgg agcggatata gttaaaagag cactaagtta ccccctaaaa ctaatcgcca
     8401 agaatgctgg tgttaatgga agtgtagtta gcgagaaggt gctttccaac gataacgtga
     8461 aattcggtta caatgctgca accggcaagt acgaggattt gatggctgca ggaatcatcg
     8521 atccaacaaa ggttgtgaga tgttgcttgg aacacgcagc ttcggttgca aagactttct
     8581 tgatgtcgga ctgcgttgtt gttgagatca aggaacctga accagttccc gtcggcaacc
     8641 caatggacaa ctcaggttac ggatactaag gtcgacgtcg accggataac aattgccctc
     8701 tagaaataat tttgtttaac tttaagaagg agatatacat atggcctctg ttgttgcccc
     8761 taagtatact tcaattaagc cattgggaga tcgagttttg gtgaagatca aggaggcaga
     8821 ggagaagact ttaggtggta tcttacttcc atccactgct caatcaaaac ctcaaggagg
     8881 tgaagtcgtt gctgtgggtg aaggaagaac tattgggaag aacaaaattg atatcactgt
     8941 ccctactgga gcacaaatta tctactccaa atacgcagga actgaggtgg agttcaatga
     9001 tgtgaagcat cttatcctca aggaagatga tattgttggc attcttgaga cagaggacat
     9061 caaagatctc aaacctttga atgaccgagt ctttattaag gttgctgagg cggaggagaa
     9121 aacagctgga gggttgttgt taaccgagac taccaaagag aagccttcta ttggcaccgt
     9181 gatagcagtt ggaccgggtt ccctagacga ggaaggtaaa attacgcctc taccagtatc
     9241 aaccggaagc acagtacttt actccaagta tgctggtaac gacttcaagg gcaaagatgg
     9301 ttccaactac attgccctca gagcttcaga tgtgatggct atactttctt agttatgtta
     9361 tatctttgta atctgcaaca agctgggtac cggtaagtta atacgactca ctatagggga
     9421 attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta agaaggagat
     9481 atacataccc atgtcaccac aaacagagac taaagcaagt gttggattca aagctggtgt
     9541 taaagagtac aaattgactt attatactcc tgagtaccaa accaaggata ctgatatatt
     9601 ggcagcattc cgagtaactc ctcaacctgg agttccacct gaagaagcag gggccgcggt
     9661 agctgccgaa tcttctactg gtacatggac aactgtatgg accgatggac ttaccagcct
     9721 tgatcgttac aaagggcgat gctaccgcat cgagcgtgtt gttggagaaa aagatcaata
     9781 tattgcttat gtagcttacc ctttagacct ttttgaagaa ggttctgtta ccaacatgtt
     9841 tacttccatt gtaggtaacg tatttgggtt caaagccctg cgcgctctac gtctggaaga
     9901 tctgcgaatc cctcctgctt atgttaaaac tttccaaggt ccgcctcatg ggatccaagt
     9961 tgaaagagat aaattgaaca agtatggtcg tcccctgttg ggatgtacta ttaaacctaa
    10021 attggggtta tctgctaaaa actacggtag agctgtttat gaatgtcttc gcggtggact
    10081 tgattttacc aaagatgatg agaacgtgaa ctcacaacca tttatgcgtt ggagagatcg
    10141 tttcttattt tgtgccgaag cactttataa agcacaggct gaaacaggtg aaatcaaagg
    10201 gcattacttg aatgctactg caggtacatg cgaagaaatg atcaaaagag ctgtatttgc
    10261 tagagaattg ggcgttccga tcgtaatgca tgactactta acggggggat tcaccgcaaa
    10321 tactagcttg gctcattatt gccgagataa tggtctactt cttcacatcc accgtgcaat
    10381 gcatgcggtt attgatagac agaagaatca tggtatccac ttccgggtat tagcaaaagc
    10441 gttacgtatg tctggtggag atcatattca ctctggtacc gtagtaggta aacttgaagg
    10501 tgaaagagac ataactttgg gctttgttga tttactgcgt gatgattttg ttgaacaaga
    10561 tcgaagtcgc ggtatttatt tcactcaaga ttgggtctct ttaccaggtg ttctacccgt
    10621 ggcttcagga ggtattcacg tttggcatat gcctgctctg accgagatct ttggggatga
    10681 ttccgtacta cagttcggtg gaggaacttt aggacatcct tggggtaatg cgccaggtgc
    10741 cgtagctaat cgagtagctc tagaagcatg tgtaaaagct cgtaatgaag gacgtgatct
    10801 tgctcaggaa ggtaatgaaa ttattcgcga ggcttgcaaa tggagcccgg aactagctgc
    10861 tgcttgtgaa gtatggaaag agatcgtatt taattttgca gcagtggacg ttttggataa
    10921 gtaatacttg gcgcgcccag tgcggccgca tgcaccggta ccgaaaggaa gctgagttgg
    10981 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
    11041 ggggtttttt gctgaaagga ggaactatat ccggatatcc cgcaagaggc ccggcagtac
    11101 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag
    11161 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact
    11221 accgcat
//
