LOCUS       sequence-31769-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector with Tet-based inducible expression of both mouse 
            G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus 
            fluorescent protein coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pSLIK-Venus-TmiR-G13-G12
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           85..206
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           234..248
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           276..402
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           461..582
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           610..624
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           652..778
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc gaatctagtc
       61 gactagggat aacagggtaa ttgtttgaat gaggcttcag tactttacag aatcgttgcc
      121 tgcacatctt ggaaacactt gctgggatta cttcttcagg ttaacccaac agaaggctcg
      181 agaaggtata ttgctgttga cagtgagcga tgggtgagtc tgtaaagtat ttagtgaagc
      241 cacagatgta aatactttac agactcaccc agtgcctact gcctcggaat tcaaggggct
      301 actttaggag caattatctt gtttactaaa actgaatacc ttgctatctc tttgatacat
      361 ttttacaaag ctgaattaaa atggtataaa ttaaatcact tttttcaatt cgcggccgca
      421 ctcgagatat ctagtcgact agggataaca gggtaattgt ttgaatgagg cttcagtact
      481 ttacagaatc gttgcctgca catcttggaa acacttgctg ggattacttc ttcaggttaa
      541 cccaacagaa ggctcgagaa ggtatattgc tgttgacagt gagcgacgac accatcttcg
      601 acaacattag tgaagccaca gatgtaatgt tgtcgaagat ggtgtcgctg cctactgcct
      661 cgcaattcaa ggggctactt taggagcaat tatcttgttt actaaaactg aataccttgc
      721 tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt
      781 tcaattcgcg gccgcactcg agatatctag acccagcttt cttgtacaaa gt
//
