LOCUS       sequence-317697-        3287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLX series: pBBR1-based T-DNA binary vector for plant transformation
            (alpha1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins.
  JOURNAL   ACS Synth Biol. 2017 Oct 20;6(10):1962-1968. doi: 
            10.1021/acssynbio.6b00354. Epub 2017 Jun 28.
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00354"; journalName: "ACS Synth Biol"; date: 
            "2017-10-20- 20"; volume: "6"; issue: "10"; pages: "1962-1968"
COMMENT     Sequence Label: pLX-B2 (GB3374)
FEATURES             Location/Qualifiers
     source          1..3287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    132..156
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    183..207
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        complement(join(526..532,533..550,551..556))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(824..1639)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1631..1650
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(1765..2427)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2428..3199
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 catcatcagt tcggtggtct tccgacgaac aataaggccg caaatcgcgg ccttttttat
       61 tgataacaaa accggctcag ttctgcgtag aaaccaacat gcaagctcca ccgggtgcaa
      121 agcggcagcg gcggcaggat atattcaatt gtaaatggct tcatgtccgg gaaatctact
      181 ggtggcagga tatattgtgg tgtaaacaat ggagaaaaag attaattaag tactagtagc
      241 ctggtcgaaa ccgtctcagg agagagacca aaagcaaaaa cccgccgaag cgggttacta
      301 gcgccattcg ccattcagag agcggagctg ctgcgacgga cgatcggtac gcgcctcttc
      361 gctattacgc caactggcga aaggtggatg tgctgcaagg cgattaagtt gggtaacgcc
      421 agggttttcc cagtcacgac gttgtagtac cacggcaagg ctatctgtaa tcattgttgt
      481 actccggtta ggacggattg ggaactggct aactcaaaat ccacacatta tacgagccgg
      541 aagcataaag tgtaaagcct ggggtgccta atgagtgagg tctctcgctg tcaggagacg
      601 ggacaaggat gcgagctagc ctgcaggaat tgttgatttt gtgatgactg atggcaggat
      661 atatgcggtt gtaattcatt tttattgtct aaatttctgt atttgtttgt ttgttcggtt
      721 gtaaattttt ttggaagaac aagaaaagaa aaaacacccg ttagggtgtt tttagttagt
      781 gtggcgcgcc gacttgcgac atgcggtcct ttgcaatcaa ctattagaaa aattcatcca
      841 gcatcagatg aaattgcagt ttgttcatat ccggattatc aatgccatat ttctgaaaca
      901 gacgtttttg caggctcggg ctaaattcgc ccaggcagtt ccacagaatg gccagatcct
      961 gataacgatc cgcaatgccc acacggccca catcaatgca gccaatcagt ttgccttcat
     1021 cgaaaatcag gttatccagg ctaaaatcgc cgtgggtcac cacgctatcc gggctaaacg
     1081 gcagcagttt atgcatttct ttccacacct gttccaccgg ccagccgtta cgttcatcat
     1141 caaaatcgct cgcatccacc aggccgttgt tcatacggct ctgcgcctgg gccagacgaa
     1201 acacacgatc gctgttaaac gggcagttgc acaccggaat gctatgcaga cgacgcagaa
     1261 acacggccag cgcatccaca atgttttcgc cgctatccgg atattcttcc agcacctgaa
     1321 acgcggtttt gcccggaatc gcggtggtca gcagccacgc atcatccggg gtgcgaataa
     1381 aatgtttaat ggtcggcagc ggcataaatt cggtcagcca gttcagacgc accatttcat
     1441 cggtcacatc gttcgccacg ctgcctttgc catgtttcag aaacagttcc ggcgcatccg
     1501 gtttgccata cagacgataa atggtcgcgc cgctctgacc cacgttatca cgcgcccatt
     1561 tatagccata cagatccgca tccatgttgc tgttcagacg cggacggcta cagctcgttt
     1621 cacgctgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     1681 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
     1741 gatcggattg gcggttatgc ggttctaccg gcgcggcagc gttacccgtg tcggcggctc
     1801 caacggctcg ccatcgtcca gaaaacacgg ctcatcgggc atcggcaggc gctgctgccc
     1861 gcgccgttcc cattcctccg tttcggtcaa ggctggcagg tctggttcca tgcccggaat
     1921 gccgggctgg ctgggcggct cctcgccggg gccggtcggt agttgctgct cgcccggata
     1981 cagggtcggg atgcggcgca ggtcgccatg ccccaacagc gattcgtcct ggtcgtcgtg
     2041 atcaaccacc acggcggcac tgaacaccga caggcgcaac tggtcgcggg gctggcccca
     2101 cgccacgcgg tcattgacca cgtaggccga cacggtgccg gggccgttga gcttcacgac
     2161 ggagatccag cgctcggcca ccaagtcctt gactgcgtat tggaccgtcc gcaaagaacg
     2221 tccgatgagc ttggaaagtg tcttctggct gaccaccacg gcgttctggt ggcccatctg
     2281 cgccacgagg tgatgcagca gcattgccgc cgtgggtttc ctcgcaataa gcccggccca
     2341 cgcctcatgc gctttgcgtt ccgtttgcac ccagtgaccg ggcttgttct tggcttgaat
     2401 gccgatttct ctggactgcg tggccatgct tatctccatg cggtaggggt gccgcacggt
     2461 tgcggcacca tgcgcaatca gctgcaactt ttcggcagcg cgacaacaat tatgcgttgc
     2521 gtaaaagtgg cagtcaatta cagattttct ttaacctacg caatgagcta ttgcgggggg
     2581 tgccgcaatg agctgttgcg tacccccctt ttttaagttg ttgattttta agtctttcgc
     2641 atttcgccct atatctagtt ctttggtgcc caaagaaggg cacccctgcg gggttccccc
     2701 acgccttcgg cgcggctccc cctccggcaa aaagtggccc ctccggggct tgttgatcga
     2761 ctgcgcggcc ttcggccttg cccaaggtgg cgctgccccc ttggaacccc cgcactcgcc
     2821 gccgtgaggc tcggggggca ggcgggcggg cttcgccctt cgactgcccc cactcgcata
     2881 ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg cgcgagcctt
     2941 gacccgcctt ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc aggccggagg
     3001 cttttcccca gagaaaatta aaaaaattga tggggcaagg ccgcaggccg cgcagttgga
     3061 gccggtgggt atgtggtcga aggctgggta gccggtgggc aatccctgtg gtcaagctcg
     3121 tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt tgtccacggg ccgagcgaag
     3181 cgagccagcc ggtggccgct cgcggccatc gtccacatat ccacgggctg gcaagggagc
     3241 gcagcgaccg cgcagggcga agcccggaga gcaagcccgt agggggc
//
