LOCUS       sequence-31770-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control lentiviral vector with Tet-based inducible expression of 
            Luciferase miR30-based shRNA, constitutive Neomycin resistance gene 
            coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pSLIK-Neo-TmiR-Luc
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           86..207
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           235..249
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           277..403
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(442..466)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc cgaatctagt
       61 cgactaggga taacagggta attgtttgaa tgaggcttca gtactttaca gaatcgttgc
      121 ctgcacatct tggaaacact tgctgggatt acttcttcag gttaacccaa cagaaggctc
      181 gagaaggtat attgctgttg acagtgagcg cccgcctgaa gtctctgatt aatagtgaag
      241 ccacagatgt attaatcaga gacttcaggc ggttgcctac tgcctcggaa ttcaaggggc
      301 tactttagga gcaattatct tgtttactaa aactgaatac cttgctatct ctttgataca
      361 tttttacaaa gctgaattaa aatggtataa attaaatcac ttttttcaat tggaagacta
      421 atgcgttttc gagatatcta gacccagctt tcttgtacaa agtggttgat aacatctcga
      481 gccataaaga tggttaatta acccacccaa gatctggcct ccgcgccggg ttttggcgcc
      541 tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga
      601 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
      661 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
      721 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
      781 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta t
//
