LOCUS       sequence-31771-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control lentiviral vector with Tet-based inducible expression of 
            mouse G beta 2 miR30-based shRNA, constitutive Neomycin resistance 
            gene coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pSLIK-Neo-TmiR-Gb2
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           85..206
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           234..248
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           276..402
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(435..459)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc gaatctagtc
       61 gactagggat aacagggtaa ttgtttgaat gaggcttcag tactttacag aatcgttgcc
      121 tgcacatctt ggaaacactt gctgggatta cttcttcagg ttaacccaac agaaggctcg
      181 agaaggtata ttgctgttga cagtgagcgc gctcatgtat tcccacgaca atagtgaagc
      241 cacagatgta ttgtcgtggg aatacatgag catgcctact gcctcggaat tcaaggggct
      301 actttaggag caattatctt gtttactaaa actgaatacc ttgctatctc tttgatacat
      361 ttttacaaag ctgaattaaa atggtataaa ttaaatcact tttttcaatt cgcggccgca
      421 ctcgagatat ctagacccag ctttcttgta caaagtggtt gataacatct cgagccataa
      481 agatggttaa ttaacccacc caagatctgg cctccgcgcc gggttttggc gcctcccgcg
      541 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
      601 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
      661 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
      721 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga tt
//
