LOCUS       sequence-31772-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control lentiviral vector with Tet-based inducible expression of 
            Luciferase miR30-based shRNA and GFP, constitutive Neomycin 
            resistance gene coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pSLIK-Neo-TGmiR-Luc
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           48..169
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           197..211
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           239..365
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(404..428)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgagctgtac aagtaaacta gtcgactagg gataacaggg taattgtttg aatgaggctt
       61 cagtacttta cagaatcgtt gcctgcacat cttggaaaca cttgctggga ttacttcttc
      121 aggttaaccc aacagaaggc tcgagaaggt atattgctgt tgacagtgag cgcccgcctg
      181 aagtctctga ttaatagtga agccacagat gtattaatca gagacttcag gcggttgcct
      241 actgcctcgg aattcaaggg gctactttag gagcaattat cttgtttact aaaactgaat
      301 accttgctat ctctttgata catttttaca aagctgaatt aaaatggtat aaattaaatc
      361 acttttttca attggaagac taatgcgttt tcgagatatc tagacccagc tttcttgtac
      421 aaagtggttg ataacatctc gagccataaa gatggttaat taacccaccc aagatctggc
      481 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
      541 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
      601 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
      661 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
      721 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
      781 tatataagga cgcgccgggt g
//
