LOCUS       sequence-317724-        7462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iPTG inducible expression of taxadiene synthase and geranylgeranyl 
            diphosphate synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7462)
  TITLE     Fox lab plasmids
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTS_TXS
FEATURES             Location/Qualifiers
     source          1..7462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(193..198,199..215,216..222)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     235..257
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(3645..3662)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3815..3901
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3993..4020
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4040..4131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4132..4200,4201..4992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4350..4369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5163..5751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5652..5671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5905..5922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5937..6077
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6003..6022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6163..6185
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        6263..6340
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6341..7423
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6360..6379)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatga gcagcagcac tggcactagc aaggtggttt
      301 ccgagacttc cagtaccatt gtggatgata tccctcgact ctccgccaat tatcatggcg
      361 atctgtggca ccacaatgtt atacaaactc tggagacacc gtttcgtgag agttctactt
      421 accaagaacg ggcagatgag ctggttgtga aaattaaaga tatgttcaat gcgctcggag
      481 acggagatat cagtccgtct gcatacgaca ctgcgtgggt ggcgaggctg gcgaccattt
      541 cctctgatgg atctgagaag ccacggtttc ctcaggccct caactgggtt ttcaacaacc
      601 agctccagga tggatcgtgg ggtatcgaat cgcactttag tttatgcgat cgattgctta
      661 acacgaccaa ttctgttatc gccctctcgg tttggaaaac agggcacagc caagtacaac
      721 aaggtgctga gtttattgca gagaatctaa gattactcaa tgaggaagat gagttgtccc
      781 cggatttcca aataatcttt cctgctctgc tgcaaaaggc aaaagcgttg gggatcaatc
      841 ttccttacga tcttccattt atcaaatatt tgtcgacaac acgggaagcc aggcttacag
      901 atgtttctgc ggcagcagac aatattccag ccaacatgtt gaatgcgttg gaaggactcg
      961 aggaagttat tgactggaac aagattatga ggtttcaaag taaagatgga tctttcctga
     1021 gctcccctgc ctccactgcc tgtgtactga tgaatacagg ggacgaaaaa tgtttcactt
     1081 ttctcaacaa tctgctcgac aaattcggcg gctgcgtgcc ctgtatgtat tccatcgatc
     1141 tgctggaacg cctttcgctg gttgataaca ttgagcatct cggaatcggt cgccatttca
     1201 aacaagaaat caaaggagct cttgattatg tctacagaca ttggagtgaa aggggcatcg
     1261 gttggggcag agacagcctt gttccagatc tcaacaccac agccctcggc ctgcgaactc
     1321 ttcgcatgca cggatacaat gtttcttcag acgttttgaa taatttcaaa gatgaaaacg
     1381 ggcggttctt ctcctctgcg ggccaaaccc atgtcgaatt gagaagcgtg gtgaatcttt
     1441 tcagagcttc cgaccttgca tttcctgacg aaagagctat ggacgatgct agaaaatttg
     1501 cagaaccata tcttagagag gcacttgcaa cgaaaatctc aaccaataca aaactattca
     1561 aagagattga gtacgtggtg gagtaccctt ggcacatgag tatcccacgc ttagaagcca
     1621 gaagttatat tgattcatat gacgacaatt atgtatggca gaggaagact ctatatagaa
     1681 tgccatcttt gagtaattca aaatgtttag aattggcaaa attggacttc aatatcgtac
     1741 aatctttgca tcaagaggag ttgaagcttc taacaagatg gtggaaggaa tccggcatgg
     1801 cagatataaa tttcactcga caccgagtgg cggaggttta tttttcatca gctacatttg
     1861 aacccgaata ttctgccact agaattgcct tcacaaaaat tggttgttta caagtccttt
     1921 ttgatgatat ggctgacatc tttgcaacac tagatgaatt gaaaagtttc actgagggag
     1981 taaagagatg ggatacatct ttgctacatg agattccaga gtgtatgcaa acttgcttta
     2041 aagtttggtt caaattaatg gaagaagtaa ataatgatgt ggttaaggta caaggacgtg
     2101 acatgctcgc tcacataaga aaaccctggg agttgtactt caattgttat gtacaagaaa
     2161 gggagtggct tgaagccggg tatataccaa cttttgaaga gtacttaaag acttatgcta
     2221 tatcagtagg ccttggaccg tgtaccctac aaccaatact actaatgggt gagcttgtga
     2281 aagatgatgt tgttgagaaa gtgcactatc cctcaaatat gtttgagctt gtatccttga
     2341 gctggcgact aacaaacgac accaaaacat atcaggctga aaaggctcga ggacaacaag
     2401 cctcaggcat agcatgctat atgaaggata atccaggagc aactgaggaa gatgccatta
     2461 agcacatatg tcgtgttgtt gatcgggcct tgaaagaagc aagctttgaa tatttcaaac
     2521 catccaatga tatcccaatg ggttgcaagt cctttatttt taaccttaga ttgtgtgtcc
     2581 aaatctttta caagtttata gatgggtacg gaatcgccaa tgaggagatt aaggactata
     2641 taagaaaagt ttatattgat ccaattcaag tatgagagct cagaaggaga tatacatatg
     2701 tttgatttca atgaatatat gaaaagtaag gctgttgcgg tagacgcggc tctggataaa
     2761 gcgattccgc tggaatatcc cgagaagatt cacgaatcga tgcgctactc cctgttagca
     2821 ggagggaaac gcgttcgtcc ggcattatgc atcgcggcct gtgaactcgt cggcggttca
     2881 caggacttag caatgccaac tgcttgcgca atggaaatga ttcacacaat gagcctgatt
     2941 catgatgatt tgccttgcat ggacaacgat gactttcggc gcggtaaacc tactaatcat
     3001 aaggtttttg gcgaagatac tgcagtgctg gcgggcgatg cgctgctgtc gtttgccttc
     3061 gaacatatcg ccgtcgcgac ctcgaaaacc gtcccgtcgg accgtacgct tcgcgtgatt
     3121 tccgagctgg gaaagaccat cggctctcaa ggactcgtgg gtggtcaggt agttgatatc
     3181 acgtctgagg gtgacgcgaa cgtggacctg aaaaccctgg agtggatcca tattcacaaa
     3241 acggccgtgc tgctggaatg tagcgtggtg tcagggggga tcttgggggg cgccacggag
     3301 gatgaaatcg cgcgtattcg tcgttatgcc cgctgtgttg gactgttatt tcaggtggtg
     3361 gatgacatcc tggatgtcac aaaatccagc gaagagcttg gcaagaccgc gggcaaagac
     3421 cttctgacgg ataaggctac atacccgaaa ttgatgggct tggagaaagc caaggagttc
     3481 gcagctgaac ttgccacgcg ggcgaaggaa gaactctctt ctttcgatca aatcaaagcc
     3541 gcgccactgc tgggcctcgc cgattacatt gcgtttcgtc agaactgagt cgacctgcag
     3601 gcatgcaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
     3661 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     3721 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     3781 tcaccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     3841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     3901 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     3961 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     4021 tgcgtttcta caaactcttt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4081 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4141 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4201 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4261 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4321 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     4381 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4441 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4501 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4561 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4621 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctacagca
     4681 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4741 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4801 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4861 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4921 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4981 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5041 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5101 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5161 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5221 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5281 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     5341 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5401 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5461 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5521 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5581 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5641 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5701 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5761 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5821 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5881 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     5941 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     6001 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     6061 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     6121 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     6181 agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg
     6241 cgaagcggca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc
     6301 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
     6361 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
     6421 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
     6481 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
     6541 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     6601 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     6661 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     6721 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     6781 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     6841 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     6901 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     6961 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     7021 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     7081 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     7141 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac
     7201 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     7261 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     7321 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7381 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7441 tgagttagcg cgaattgatc tg
//
