LOCUS       sequence-31773-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control lentiviral vector with Tet-based inducible expression of 
            mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin 
            resistance gene coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pSLIK-Neo-TGmiR-Gb2
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           47..168
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           196..210
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           238..364
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(397..421)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctgtaca agtaaactag tcgactaggg ataacagggt aattgtttga atgaggcttc
       61 agtactttac agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca
      121 ggttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gcgctcatgt
      181 attcccacga caatagtgaa gccacagatg tattgtcgtg ggaatacatg agcatgccta
      241 ctgcctcgga attcaagggg ctactttagg agcaattatc ttgtttacta aaactgaata
      301 ccttgctatc tctttgatac atttttacaa agctgaatta aaatggtata aattaaatca
      361 cttttttcaa ttcgcggccg cactcgagat atctagaccc agctttcttg tacaaagtgg
      421 ttgataacat ctcgagccat aaagatggtt aattaaccca cccaagatct ggcctccgcg
      481 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
      541 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
      601 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
      661 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
      721 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gatta
//
