LOCUS       sequence-31774-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector for cloning miR30-based shRNA driven by human U6 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_hUmiRc2
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..129
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     ncRNA           242..363
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             399..704
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRIVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     583..602
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     ncRNA           725..851
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
       61 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      121 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      181 aaacaccgtg ctcgcttcgg cagcacatat actagtcgac tagggataac agggtaattg
      241 tttgaatgag gcttcagtac tttacagaat cgttgcctgc acatcttgga aacacttgct
      301 gggattactt cttcaggtta acccaacaga aggctcgaga aggtatattg ctgttgacag
      361 tgagcgtgct gcaggtaagc ttgaggtgtg cttccagaat gcagtttaag gtttacacct
      421 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
      481 ccgggcgacg gatagtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
      541 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
      601 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
      661 atgacatcaa aaacgccatt aacctgatgt tctggggaat atagaattcc aacctgctgc
      721 ttgcctactg cctcggaatt caaggggcta ctttaggagc aattatcttg tttactaaaa
      781 ctgaatacct tgctatctct ttgatacatt tttacaaagc tgaattaaaa tggtataaat
      841 taaatcactt ttttcaattg g
//
