LOCUS       sequence-31775-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector for cloning  miR30-based shRNA driven by TRE promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_TmiRc3
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           124..245
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             281..586
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRIVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     465..484
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     ncRNA           607..733
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaacta
       61 ggcctaggcg tctgatcact agtgactcta gtcctagtcg actagggata acagggtaat
      121 tgtttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg gaaacacttg
      181 ctgggattac ttcttcaggt taacccaaca gaaggctcga gaaggtatat tgctgttgac
      241 agtgagcgtg ctgcaggtaa gcttgaggtg tgcttccaga atgcagttta aggtttacac
      301 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
      361 gcccgggcga cggatagtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
      421 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
      481 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
      541 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atatagaatt ccaacctgct
      601 gcttgcctac tgcctcggaa ttcaaggggc tactttagga gcaattatct tgtttactaa
      661 aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa
      721 attaaatcac ttttttcaat tggaagacta atgcgttttc gagatatcta gacccagctt
//
