LOCUS       sequence-317763-        4409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses heterlogous ribosomal proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Kolber NS, Fattal R, Bratulic S, Carver GD, Badran AH
  TITLE     Orthogonal translation enables heterologous ribosome engineering in 
            E. coli.
  JOURNAL   Nat Commun. 2021 Jan 26;12(1):599. doi: 10.1038/s41467-020-20759-z.
  PUBMED    33500394
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-26"; volume: "12(1):599. doi"; pages: " 
            10.1038/s41467-020-20759-z"
COMMENT     Sequence Label: pAB184a
FEATURES             Location/Qualifiers
     source          1..4409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      complement(902..933)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(949..1608)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(1723..2673)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2721..2943
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(3505..4383)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttacctgc
      301 aggtgcagtt tctttcagga gattccaata tggcaaacag cgcacaagca cgcaaacgtg
      361 cccgccagtc cgtcaaacaa cgcgcccaca acgctagcct gcgtaccgca ttccgcaccg
      421 cagtgaaaaa agtattgaaa gcagtcgaag caggcgataa agctgccgca cgagcggttt
      481 accaagagtc cgtcaaagtc atcgaccgca tcgccgacaa aggcgtgttc cacaaaaaca
      541 aagcggctcg ccacaaaagc cgcctgtctg caaaagtaaa agcactggct tgatttttgc
      601 attttctcaa aaggactcaa aatggtagtt atccgtttgg cacgcggcgg ctcgaaacac
      661 cgccccttct acaacgtcat cgttactgac tcacgcagcc gccgcgacgg ccgcttcatc
      721 gaacgcgtag gcttctacaa ccccgtagcc aacgaaaaac aagagcgcgt ccgcctcaat
      781 gcagaccgcc tgaaccactg gattgcacaa ggcgcgcaag tcagcgactc cgttgcaaaa
      841 ctgattaaag aacaaaaagc cgtctaatcc gcgttactta attaacggca ctcctcagcc
      901 aagtcaaaag cctccggtcg gaggcttttg actacatgcc catggcgttt acgccccgcc
      961 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     1021 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     1081 atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     1141 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     1201 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     1261 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     1321 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     1381 tgccatacgg aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     1441 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     1501 ggtctggtta taggtacatt gagtaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     1561 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     1621 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     1681 gtgaaagttg gaacctctta cgtgccaggc caaataggcc gttcagatcc ttccgtattt
     1741 agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt
     1801 gagatcatgc ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat
     1861 ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttacgtaggt aggaatctga
     1921 tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt
     1981 tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc
     2041 tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt
     2101 caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa
     2161 cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc
     2221 ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc
     2281 cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa
     2341 aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatcttaaa
     2401 gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt
     2461 agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt attggttata
     2521 agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc
     2581 cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag
     2641 ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta
     2701 atcactatac caattgagat gggctagtca atgataatta ctagtccttt tcctttgagt
     2761 tgtgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc
     2821 ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat
     2881 ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa
     2941 ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc
     3001 tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa
     3061 atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg
     3121 acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc
     3181 gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct
     3241 tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc
     3301 agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc
     3361 agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg
     3421 tcaagaggac atccggtcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     3481 ttgctgagga gacttaggga ccctttatga caacttgacg gctacatcat tcactttttc
     3541 ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga
     3601 gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt
     3661 ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct
     3721 aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg
     3781 tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc
     3841 ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg
     3901 cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg
     3961 cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg
     4021 gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc
     4081 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta
     4141 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg
     4201 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat
     4261 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc
     4321 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc
     4381 catacttttc atactcccac cattcagag
//
