LOCUS       sequence-317764-        6466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses heterlogous ribosomal proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Kolber NS, Fattal R, Bratulic S, Carver GD, Badran AH
  TITLE     Orthogonal translation enables heterologous ribosome engineering in 
            E. coli.
  JOURNAL   Nat Commun. 2021 Jan 26;12(1):599. doi: 10.1038/s41467-020-20759-z.
  PUBMED    33500394
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-26"; volume: "12(1):599. doi"; pages: " 
            10.1038/s41467-020-20759-z"
COMMENT     Sequence Label: pAB184b3
FEATURES             Location/Qualifiers
     source          1..6466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..879
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     rep_origin      1441..1663
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1711..2661
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             2776..3435
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVLTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      3451..3482
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        complement(6156..6440)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(6214..6233)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 atggctgaag cgcaaaatga tcccctgctg ccgggatact cgtttaatgc ccatctggtg
       61 gcgggtttaa cgccgattga ggccaacggt tatctcgatt tttttatcga ccgaccgctg
      121 ggaatgaaag gttatattct caatctcacc attcgcggtc agggggtggt gaaaaatcag
      181 ggacgagaat ttgtttgccg accgggtgat attttgctgt tcccgccagg agagattcat
      241 cactacggtc gtcatccgga ggctcgcgaa tggtatcacc agtgggttta ctttcgtccg
      301 cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat ttgccaatac ggggttcttt
      361 cgcccggatg aagcgcacca gccgcatttc agcgacctgt ttgggcaaat cattaacgcc
      421 gggcaagggg aagggcgcta ttcggagctg ctggcgataa atctgcttga gcaattgtta
      481 ctgcggcgca tggaagcgat taacgagtcg ctccatccac cgatggataa tcgggtacgc
      541 gaggcttgtc agtacatcag cgatcacctg gcagacagca attttgatat cgccagcgtc
      601 gcacagcatg tttgcttgtc gccgtcgcgt ctgtcacatc ttttccgcca gcagttaggg
      661 attagcgtct taagctggcg cgaggaccaa cgtatcagcc aggcgaagct gcttttgagc
      721 accacccgga tgcctatcgc caccgtcggt cgcaatgttg gttttgacga tcaactctat
      781 ttctcgcggg tatttaaaaa atgcaccggg gccagcccga gcgagttccg tgccggttgt
      841 gaagaaaaag tgaatgatgt agccgtcaag ttgtcataaa gggtccctaa gtctcctcag
      901 caaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgaccg gatgtcctct
      961 tgacagtaag acgggtaagc ctgttgatga taccgctgcc ttactgggtg cattagccag
     1021 tctgaatgac ctgtcacggg ataatccgaa gtggtcagac tggaaaatca gagggcagga
     1081 actgctgaac agcaaaaagt cagatagcac cacatagcag acccgccata aaacgccctg
     1141 agaagcccgt gacgggcttt tcttgtatta tgggtagttt ccttgcatga atccataaaa
     1201 ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagcgca gcgaactgaa
     1261 tgtcacgaaa aagacagcga ctcaggtgcc tgatggtcgg agacaaaagg aatattcagc
     1321 gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg tctgttttgt
     1381 agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga ctaaagtagt
     1441 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
     1501 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
     1561 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
     1621 acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa ttggtatagt
     1681 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
     1741 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
     1801 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
     1861 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
     1921 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
     1981 ggctttaaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
     2041 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
     2101 ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta
     2161 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
     2221 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
     2281 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
     2341 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
     2401 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     2461 acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc tttaactgca
     2521 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagcatgat
     2581 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg
     2641 gctaaatacg gaaggatctg aacggcctat ttggcctggc acgtaagagg ttccaacttt
     2701 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
     2761 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     2821 tggcatcgta aagaacattt tgaggcattt cagtcagtta ctcaatgtac ctataaccag
     2881 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     2941 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg
     3001 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     3061 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     3121 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     3181 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     3241 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttctcac tatgggcaaa
     3301 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     3361 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     3421 cagggcgggg cgtaaacgcc atgggcatgt agtcaaaagc ctccgaccgg aggcttttga
     3481 cttggctgag gagtgccgtt aattaagtct cgtcgatcac ttgcgcagac cgagtttttc
     3541 gatcagtgcg cggtacgaat cgggattgcg gcccttgaga tagtcgagca gcttgcgacg
     3601 acggctgacc atgcgcagca ggccgcggcg cgagtggtgg tccttcatgt gttctttgaa
     3661 gtgaccggtc agttcgttga tgcgagcggt aagcagtgcc acctgaactt caggggagcc
     3721 ggtatcgcct tgagcgcgtt ggaattgcgc gacgatttcg gattttttga tgtcagctac
     3781 agacatttga tgacctctac ggacattggt cacgtcaacc gtcgttgcgc tgttggtcca
     3841 gcttggcctt gagcagcgcg cccaagttcg tcgtacccga cgaagcgctc gcctcggaca
     3901 tgcgctggat ggtctcggcc gtctcggcgt tatcgcgggc cttgatcgac agctggatcg
     3961 aacgcgcctt gcggtcgatg ttgacgatca tggcttcgat gttctcgcca gccttcagca
     4021 cggtggtagc gtcttcgacg cggcccgagg agatctcgga ggcgcgcagg tagccttcca
     4081 catccaccga cagggtgatc acggcgccct tgggctcgac cgacttgatg gtgcccggaa
     4141 caacggcgcc cttgtcgtgc gtggcaacga agttgttgaa cgggtcgcct tcgagctgct
     4201 tgatacccag cgagatgcgc tctttctcgg tatcgatgcc cagcaccacg gcttccagct
     4261 cgtcgccctt cttgaagttg cgcacggctt cctcgcccga ttccgtccac gacaggtcgg
     4321 acagatggac caggccgtcg atgccgccgg gcaggccgac gaacacgccg aagtcggtga
     4381 tcgacttgat ggcgccgcgg accttgtcac cacgcttgaa gttggtggcg aactcttccc
     4441 acgggttctg gcggcactgc ttcatgccca gcgaaatgcg gcgacggtct tcgtcgattt
     4501 ccaggaccat gacttcgact tcttcgccca gggtcacgac cttgcgcgga tcgacgttct
     4561 tgttggtcca gtccatttcg gacacgtgca ccaggccttc gatgccggct tcgacttcga
     4621 cgaacgcgcc gtagtcggtc aggttggtga ccttgccgaa caggcgggtg ccctgcgggt
     4681 agcggcgagc caggcccacc cacggatctt cgcccagctg cttgacgccc agggagacgc
     4741 ggctcttttc ctggtcgaac ttgagcacct tggcttcgac ttcctgaccc acttgcagga
     4801 cttcggacgg gtgacgcaca cggcgccagg ccatgtcggt gatgtgcagc aggccatcga
     4861 tgccgcccag gtcgacgaac gcgccgtagt cggtgatgtt cttgaccacg cccttgacca
     4921 ccgcgccttc gtgcagcgtc tcgagcagct tctggcgctc ttcgcccatg ctggcttcca
     4981 gcacctggcg gcgcgacagc acgacgttgt tgcgcttgcg gtccagcttg atgaccttga
     5041 attcgagggt cttgccttcg tacggcgtgg tgtccttgac cggacgcagg tcgaccagcg
     5101 aaccgggcag gaacgcgcgg atgccgttgg tcatgacggt caggccgccc ttgaccttgc
     5161 cggtgatcgt gccggtgacc agctcgccgt tctcgagggc ctgctccagt tgcagccagg
     5221 ccgacagacg cttggcgcgg tcgcgcgaca ggatggtgtc gccgtagccg ttctccagcg
     5281 aatcgatcgc caccgagacg aagtcgccgg gttgaacttc gagttcgccc tggtcgttga
     5341 ggaactcttc caggggaatc agcgcttcgg acttcaggcc ggcgttgacg accacgaagt
     5401 tgtggtcgac gcgcacgact tctgcgctga tgacctcgcc ggacttcatg tcctggctct
     5461 tgaggctttc tgcgaacagg tcggcgaagt tttcgccgcc aaggatggcg gaggtggaaa
     5521 cggaagacat taggttggaa tccatttggc cagcgggctc aggcagcagc cgaaaccttg
     5581 gccgagtatt ccttgaccag gcgctcgacg gcgggcgaca gcagcgcgcc gttgccggtc
     5641 cagtattgca cgcggtccag ggcgatgcgc agattctcgg tgccttcagc ggcaaccggg
     5701 ttgtaaaagc caacgcgctc gacgaaacgg ccatcgcgac gattgcgcga atcggtagct
     5761 accaggttgt aaaacggacg cttcttcgag ccaccgcggg ccagacgaat caccagcata
     5821 ggtaatccct tgaattggtt acttgcttta ggccagcgcc ttgacggcgg cagccaggcg
     5881 gctcttgtgg cgagcagcct tgttcttgtg gatgatgttc ttgtcagcca cgctgtcaat
     5941 cacgctcgtg gctttacgca gcgtttcgcc agcagcagcc ttatcgccag tggcgatgga
     6001 ttggcgaacg cgcttgatgg cggtgcgcag catcgagcgc aggctggagt tgtgcttgtt
     6061 gcgctggacc gattggcgag cgcgcttgcg ggcttgggcg gtattggcca tgttgcttag
     6121 ctagtatgaa tactgcacct gcaggtaaaa cgggtatgga gaaacagtag agagttgcga
     6181 taaaaagcgt caggtaggat ccgctaatct tatggataaa aatgctatgg catagcaaag
     6241 tgtgacgccg tgcaaataat caatgtggac ttttctgccg tgattataga cacttttgtt
     6301 acgcgttttt gtcatggctt tggtcccgct ttgttacaga atgcttttaa taagcggggt
     6361 taccggttgg gttagcgaga agagccagta aaagacgcag tgacggcaat gtctgatgca
     6421 atatggacaa ttggtttctt ctctgaatgg tgggagtatg aaaagt
//
