LOCUS       sequence-317765-        6469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses heterlogous ribosomal proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Kolber NS, Fattal R, Bratulic S, Carver GD, Badran AH
  TITLE     Orthogonal translation enables heterologous ribosome engineering in 
            E. coli.
  JOURNAL   Nat Commun. 2021 Jan 26;12(1):599. doi: 10.1038/s41467-020-20759-z.
  PUBMED    33500394
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-26"; volume: "12(1):599. doi"; pages: " 
            10.1038/s41467-020-20759-z"
COMMENT     Sequence Label: pAB184c3
FEATURES             Location/Qualifiers
     source          1..6469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      complement(2962..2993)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(3009..3668)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(3783..4733)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4781..5003
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(5565..6443)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttacctgc
      301 aggtgcagta aacttaggct ttattaaaaa tggctaatac cgcacaagca cgcaagcgcg
      361 cccgccaagc agtggctcgc aacaaacaca acgccagcat ccgttccatg ctgcgtaccg
      421 ctatcaagcg cgttcgccag gctatcgaag ccggtgacaa aactgctgct aacgaagttt
      481 tccgcaaagc gaccagcatc attgatcgcg ttgccgacaa gaacatcatt cacaagaaca
      541 aggctgcccg ccacaagagc cgcctgtctg ctgctatcaa ggccctggcc tgattgctga
      601 aacacaataa agggattatc aatggtggta attcgcttgg cccgtggtgg ctccaaaaag
      661 cgtccttttt acaacgtagt agccgccgag gcgagccaac gccgtgatgg tcgcttcatc
      721 gaacgtctgg gtttctacaa ccccgtcgct ggcgaaggtc atgaaaacct gcgtctgcaa
      781 atggaccgcg ttaagcactt caccgacaac ggtgctcagg tttcgcctgc tgtggctcgc
      841 ctgttgaaag aatactcggc taaggttgct tcggctgcct aagttgattc ttggctaacg
      901 gattgattta atgtccacta accttcacac agacgcaacg ggcggcgaaa gctttgccga
      961 tttgtttgca gaaagcatta aaaaccaaga cctgaaaagc ggcgaagtta tctccgccga
     1021 ggttctgcgt attgaccaca actacgttct ggtcaatgct ggcctgaagt ccgaagcact
     1081 gatccctctg gaagagttcc tgaacgatca gggcgagctg gaagttcaag ctggtgactt
     1141 tgtttccgtg gcaatcgacg cctttgaaaa cggctacggc gacaccatcc tgtcccgcga
     1201 ccgcgccaag cgtctgtcgg cctggttgtc cctcgagcaa gcgcttgagt ccggcgaact
     1261 ggtcaacggt accatcactg gtaaggtcaa gggcggtctg actgtcatga ccaacggcat
     1321 ccgtgcattc ttgcccggtt cgctggttga tctgcgtcct gtcaaggaca ccacgccata
     1381 cgaaggcaag accctggaat tcaaggttat caagctcgat cgcaagcgta acaacgtcgt
     1441 gttgtcgcgt cgcgctgtgc tggaagcctc catgggcgaa gagcgtcaga aactgctgga
     1501 aaccctgtcc gaaggcgcta tcgtcaaagg tatcgtcaag aatatcaccg attacggtgc
     1561 gttcgtggat ctgggcggca tcgacggtct gctgcacatc accgacatgg catggcgccg
     1621 tgttcgtcac ccatccgaag ttctgcaggt gggtcaggaa atcgaagcca aggtcctcaa
     1681 gttcgatcag gaaaagagcc gcgtctccct gggcgtcaag cagctgggcg aagatccatg
     1741 gatcggtctg gcacgtcgct acccacaaaa cacccgtttg ttcggcaagg ttaccaacct
     1801 gaccgactac ggtgcatttg ttgaagtgga agacggtatc gaaggtctgg ttcacgtatc
     1861 cgaaatggat tggaccaaca agaacgttga cccacgcaag gttgtgacct tgggtgaaga
     1921 agtcgaagtc atggttctgg aaatcgacga agatcgtcgt cgtatctcct tgggcatgaa
     1981 gcagtgccgt gccaacccat gggaagattt cgccatcaac ttcaagcgcg gcgacaaggt
     2041 tcgcggtggc atcaagtcca tcaccgactt cggcgtgttt gttggtctgc caggcggcat
     2101 cgacggtctg gttcacctgt ccgacctgtc ctggtccgag actggcgaag aagccgttcg
     2161 caacttcaag aagggcgacg aagttgacgc cgtggttctg ggcatcgaca ccgacaaaga
     2221 gcgcatttcc ctgggtatca aacagctgga aggcgatccg ttcaacaact tcgtagctac
     2281 ctacgacaag ggtgctgtag ttagcggcgt cgtcaagtcg gtagaagcca agggtgctgt
     2341 tgttactctg tcgctggacg ttgaaggcta cctgcgcgcc tccgagatct cctcgggtcg
     2401 tgttgaagat gccacgacgg ctctgaaaga aggtcaggaa atcgaaacca tgatcttgag
     2461 cgtggatcgc aagacccgtt cgattcagtt gtccatcaag gctcgtgata ccgccgaaac
     2521 cgctcaatcc atggcccgcc tgaccgaagc cagcgcttcg tccggtacga ccaatctggg
     2581 tgctctgctg cgtgccaagc tggaccagca gcaacgtgac gacggttaat cagcgtgagt
     2641 cttagaggct aaataaaatg gcagttgcag atatcaaaaa agtcgatatc gtttccgagt
     2701 ttcagcgtaa acaaggcgat acaggctccc cagaagtgca agtggctttg ctgaccgctc
     2761 gcatcaacga attgaccggc cacttcaagg ctcacaacaa ggatcaccac tcccgtcgtg
     2821 gtctgttgcg catggttagc cgtcgtcgca aattgctgga ttacctgaaa ggtcgtaatc
     2881 cagacgctta ccgtgctttg atcgaaaaac tcggtctgcg taagtgatac cggggactta
     2941 attaacggca ctcctcagcc aagtcaaaag cctccggtcg gaggcttttg actacatgcc
     3001 catggcgttt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     3061 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     3121 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc
     3181 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     3241 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     3301 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     3361 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     3421 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca
     3481 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     3541 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagtaactga ctgaaatgcc
     3601 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     3661 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     3721 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccaggc caaataggcc
     3781 gttcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     3841 agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa aaattttgcc
     3901 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     3961 ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     4021 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     4081 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     4141 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     4201 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     4261 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     4321 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     4381 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     4441 gtccactgga aaatcttaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     4501 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     4561 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     4621 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     4681 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     4741 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     4801 ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa
     4861 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     4921 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     4981 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     5041 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     5101 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     5161 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     5221 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     5281 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     5341 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     5401 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     5461 caggcttacc cgtcttactg tcaagaggac atccggtcaa ataaaacgaa aggctcagtc
     5521 gaaagactgg gcctttcgtt ttgctgagga gacttaggga ccctttatga caacttgacg
     5581 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
     5641 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
     5701 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
     5761 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac
     5821 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
     5881 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
     5941 ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc
     6001 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
     6061 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
     6121 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
     6181 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
     6241 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
     6301 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
     6361 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
     6421 ggatcatttt gcgcttcagc catacttttc atactcccac cattcagag
//
