LOCUS       sequence-317768-        6409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses heterlogous ribosomal proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Kolber NS, Fattal R, Bratulic S, Carver GD, Badran AH
  TITLE     Orthogonal translation enables heterologous ribosome engineering in 
            E. coli.
  JOURNAL   Nat Commun. 2021 Jan 26;12(1):599. doi: 10.1038/s41467-020-20759-z.
  PUBMED    33500394
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-26"; volume: "12(1):599. doi"; pages: " 
            10.1038/s41467-020-20759-z"
COMMENT     Sequence Label: pAB184e2
FEATURES             Location/Qualifiers
     source          1..6409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(695..1573)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1600..1884
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1807..1826
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      complement(4501..4532)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(4548..5207)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(5322..6272)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      join(6320..6409,1..133)
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag
       61 tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc
      121 ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc
      181 tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa
      241 gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt
      301 ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg
      361 cactacaggc gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc
      421 gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt
      481 gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga
      541 caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc
      601 cgtcttactg tcaagaggac atccggtcaa ataaaacgaa aggctcagtc gaaagactgg
      661 gcctttcgtt ttgctgagga gacttaggga ccctttatga caacttgacg gctacatcat
      721 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa
      781 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag
      841 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc
      901 ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc
      961 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt
     1021 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt
     1081 ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc
     1141 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg
     1201 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat
     1261 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat
     1321 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa
     1381 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat
     1441 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg
     1501 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt
     1561 gcgcttcagc catacttttc atactcccac cattcagaga agaaaccaat tgtccatatt
     1621 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc caaccggtaa
     1681 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta
     1741 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac
     1801 tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga cgctttttat
     1861 cgcaactctc tactgtttct ccatacccgt tttacctgca ggtgcagtca aaactccaag
     1921 gagttatcgt ggccaacatc aagtctgccc agaagcgtgc gcttcaatcc gaaaaacgtc
     1981 gtctgcacaa caacagccta cgttctaaag tacgtaccag catcaagcgt gtacttaaag
     2041 aagtacaaac tggtgactgg gaaaaagcta tggctgcttt ccaagctgca cagccagtta
     2101 ttgatcgcat tgctgacaag ggtgttttca acaagaactg ggctgctcgt actaagagcc
     2161 gtctgaacgc tcgtgttaaa gcacttaaag cttcctaagt tttaggtttt aaaggaaaga
     2221 ctcgtaatgg taaccattcg tttagcacgt ggtggcgcaa aaaagcgtcc gttttatcac
     2281 ctacacgtaa ctgattcacg taatgcacgt gatggtcgtt acattgagcg cctaggtttc
     2341 tttaaccctg tagctcaagg taatgcagag cgtctacgta ttgatactga tcgtctacag
     2401 cactgggttg ctcaaggcgc acagttgtca gagcgtgttg ctaagttagt aaaagaagca
     2461 gctaagcagc agtaattact gcttaaaatc gagtaaataa tacatgacac aatcctttgc
     2521 cgaactcttt gaagaaagct tgcaaacagt caatatggaa gtgggctcta tcattaaagc
     2581 cactattgtt gatgttgatg gtgactgggt taccgttaac gctggcttaa agtcggaagg
     2641 tgttatccac cgcaatcagt ttgaaagcgg tgaagatcta accgttggtt cacaagtgct
     2701 agtagcacta gaaaccgtag aagatggttt cggtgaaacc cgtctatccc gtgaaaaagc
     2761 acgtcgtgct gagtcttgga ttgagctaga agaagctttc gagaagagcg aaatcgtcaa
     2821 gggtattatc aatggcaagg tcaagggtgg ctttacagtt gatatcaata ccatccgtgc
     2881 cttcctacca ggttcacttg ttgatgtacg cccagtgcgt gacacagcac acctagaata
     2941 caaagaactc gatttcaaag ttatcaagct agatgctaag cgtaacaacg tggttgtttc
     3001 tcgccgtgcc gtactagaag cagaaaactc agcagagcgt gaagaactgc ttgctaacct
     3061 agctgaaggc caagaagtta aaggtatcgt taagaacctt actgattacg gtgccttcgt
     3121 tgacttgggc ggcgtagacg gtctactaca catcactgat atggcttgga agcgtattaa
     3181 gcatccttct gaaattgttg ctgtgggtga tgaaatcaac gttaaggttc ttaagtttga
     3241 tcgcgagcgt aaccgtgtat cattaggtct taagcagttg ggtgaagatc catggattca
     3301 aattaaagca cgttaccctg agtcttcaat cattaaggca cgtatcacta acctaaccga
     3361 ttacggttgc ttcgctgagc tagaagaagg cgttgaaggt ttagtacacg tttctgaaat
     3421 ggactggact aacaaaaacg ttaatcctaa gcaggttgtt aacctaggtg acgaagtaga
     3481 agtaatgatt ctagacatcg acgaagaacg tcgtcgtatt tctctaggta tcaagcagtg
     3541 ccgcgctaat ccttgggaag agttctcaag caagtacaac aaaggcgaca aagtttctgg
     3601 cgctatcaag tcaatcactg acttcggtat ctttgttggc ttaaatggcg gtatcgatgg
     3661 tctagttcac ctgtctgata tttcttggaa tgatactggg gaagaagccg tacgtaagtt
     3721 caagaaaggc gaagaagttg aaacaatcat tctttcgatt gacccagagc gtgagcgtat
     3781 ctcgctaggt gttaagcaac ttgaaagcga tcctttcaat gagttcttag cactaaacga
     3841 caaaggctct atcgttaaag gtaaggtttc tgcagttgaa gctaaagaag ccgttgttga
     3901 cctaacagat gaagtgcaag cgattctaaa agcatctgaa attagtgctg agcgtgttga
     3961 agatgcgcgt aatgctctaa aagaaggcga tgaagttgaa gcacgtatta tcaacgttga
     4021 tcgtaagaat cgtgtgatta gcttgtctat taaagccaaa gataaagttg aagctgaaca
     4081 agcggttgct actcagcgtg aagctcaggc tgaacttgca ggtccaacca ctattggtga
     4141 cctaatcaag caaaaaatgg aaaataacta atactaatca gcgactgtgg agttttaaaa
     4201 tgttaactac tgaacgtaaa gctgaaatcg taaaagaatt cgcgcgtgct gaaaatgata
     4261 ccggctcacc tgaagtacag gttgccttgc taacttttga tatcaatgca ctacaatctc
     4321 acttcgaaac taacaaaaaa gatcaccaca gccgtcgtgg tctaattcgt aaggttagtc
     4381 agcgtcgtaa gctgctagac tacttgaaga aaaaagacgt aacgcgttat gcagatctaa
     4441 ttaagcgttt aggtctacgt cgctaagtta tagaacttaa ttaacggcac tcctcagcca
     4501 agtcaaaagc ctccggtcgg aggcttttga ctacatgccc atggcgttta cgccccgccc
     4561 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     4621 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     4681 tatttgccca tagtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     4741 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     4801 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     4861 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     4921 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     4981 gccatacgga actccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     5041 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     5101 gtctggttat aggtacattg agtaactgac tgaaatgcct caaaatgttc tttacgatgc
     5161 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     5221 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     5281 tgaaagttgg aacctcttac gtgccaggcc aaataggccg ttcagatcct tccgtattta
     5341 gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg
     5401 agatcatgct tactttgcat gtcactcaaa aattttgcct caaaactggt gagctgaatt
     5461 tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tacgtaggta ggaatctgat
     5521 gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc aagttcggtt
     5581 acgagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt cgggcggcct
     5641 cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact tattggtttc
     5701 aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat taacatgaac
     5761 ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg tgttagttct
     5821 tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt aacatgttcc
     5881 agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc ttcactaaaa
     5941 actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa aatcttaaag
     6001 cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct ggttgcttta
     6061 gctaatacac cataagcatt ttccctactg atgttcatca tctgagcgta ttggttataa
     6121 gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt gagtagtgcc
     6181 acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact aatcgctagt
     6241 tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag gtgattttaa
     6301 tcactatacc aattgagatg ggctagtcaa tgataattac tagtcctttt cctttgagtt
     6361 gtgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaatt
//
