LOCUS       sequence-317770-        9498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018349
FEATURES             Location/Qualifiers
     source          1..9498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     216..235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     469..486
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    603..624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(639..644,645..662,663..669)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    677..693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     682..704
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     701..717
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     701..717
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     RBS             1403..1425
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3280..3300
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3301..3324
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    3350..3406
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3407..3424)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3407..3423)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3416..3438)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3632..3651)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3751..3773
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3811..3829)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             4615..5814
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(6045..7193)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7465..7836)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            7869..7978
                     /note="incP origin of transfer"
     rep_origin      8131..8762
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      join(9225..9498,1..315)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
       61 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      121 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      181 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      241 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      301 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      361 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
      421 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
      481 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      541 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      601 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      661 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      721 gattacgcca agctagcttc ccgcttggtc gggccgcttc gcgagggccc gttgacgaca
      781 acggtgcgat gggtcccggc cccggtcaag acgatgccaa tacgttgcga cactacgcct
      841 tggcactttt agaattgcct tatcgtcctg ataagaaatg tccgaccagc taaagacatc
      901 gcgtccaatc aaagcctaga aaatataggc gaagggacgc taataagtct ttcataagac
      961 cgcgcaaatc taaaaatatc cttagattca cgatgcggca cttcggatga cttccgagcg
     1021 agcctggaac ctcagaaaaa cgtctgagag ataccgcgga tctcacacag gaaatttcgc
     1081 aacgcaaaat gctgaattta gcaggcaagt ttcctgataa agggccctgt gagtgcgaca
     1141 ttcgacgcgc gaaagcgagt tgacggcgcc cgaaattttc ttagagttcg ttcaagcttc
     1201 ggtttcgggg agccgctaag ggatgcggac ccagccgggt aagtcgccgg gaactatgga
     1261 ataatcctaa tttctgtgcg gcaggcgcga gctgccggag cgaatatcat ccctgtcgca
     1321 aacagcaggt caatcaacgg tgggtagcgg gctgctatcc agcggcggat aagacttgtc
     1381 ggaggaatcc tctagaaata attttgttta actttaagaa ggagatatac catggggatc
     1441 gagcagtgga cccgcggccg ccagcgcggg cacggctgcg gcttccgcac ctcgtggatc
     1501 taccccctca cgatcctcct cgccatgccg ttctctcttt gggccaacga ggagctgctt
     1561 cgtctcggcg ccgacccggg gcagtggccc atgcaggcca aaaactacgc cgccacccgg
     1621 tactcggagc tggaccagat caacgcccgc accgtggagc gcctccgcgt ggcgtggtcc
     1681 ttctcgacgg gggccctgcg gggccacgag ggcggcccgc tggtggtggg cggcatcatg
     1741 tacgtccata gcccgttccc caacaccgtc tatgccctcg acctcgctaa gaagccgtac
     1801 gccatcctct ggaagtacac cccggagcag aagcccacgg cggtgaccga ggcgtgctac
     1861 gacaccgtca atcgcggggt cgcctacggg atggggaaga tcttcctcgg cgccctcgac
     1921 ggcgagctga tcgccctcga cgcccacacc ggggccgagg tctggaaggc caagcaggga
     1981 gagatcgggc gcggcgagac catcacgggt gcccccattg tgatcaagga catggtgctg
     2041 gtgggcatct cggggggcga gttcggcgtc cgcggccgcg tcaatgccta cgatgccaac
     2101 acgggcaagc cccggtgggt tgcgtattcg cagggaccgg acgacgatgt cctgctcgag
     2161 ggcaacttca acaaggagtg cccgcgacac gggggggagg gcgagggcac ccgctcgtgg
     2221 cccggcgagc agtggaagct cggcggcggc accacctggg ggtggttctc ctacgatccg
     2281 gacctcgatc tgttccacta cgggtccggc aaccccgggt cctggaaccc cgacgaacgc
     2341 aaggggggcg acaacaagtg ggcgtgcacc ctgtgggggc gtcgcccagc caccgggatg
     2401 gcccgttggg cgtaccagat gaccccttgg gattcctggg gctacgatgg gacgtcggag
     2461 ctcgtgctgg tggacctgcg catcggctcg acgctgcgga agtgcgcggt gcacttcgac
     2521 aagaatggct atgcgtacac ggtcgaccgg ggcaccggcg aactcctctc agcggcaccc
     2581 ttcgtctacg tgaactgggc caagggcatc tcccggggtg atgatcggcc aatcgaagtc
     2641 ccggagaagc gcgcaaaggc gggcgtcgac atcaagggcg tgtgcccgag cgccatgggc
     2701 ggcaagaatc agcagccggc ggcgtacgac ccggagaccc acctcttcta tgtgggaacc
     2761 aacaacctgt gcatggatat cctcgccacc gaggtgcgct atatctcggg ctctccctac
     2821 atcggcgccg atctcaagat ctactccggg cacgagggcc aggacaagta ctatggcgag
     2881 ttcatcgcct gggacccggt caaggcccgc cgcgcgtggg agatccagga gcgcttcccg
     2941 gtgtgctccg gcgccctcgc gacgtcgggc gggctcgtgt tttacggtac gctggatggc
     3001 tggttcaagg cggtcgacgc tcggaccggc gtggttctct ggcagcagaa gctgggctcc
     3061 ggcatcgtcg gctcccccat cgccttcctg ggccccgaca acaaggagta cgtcgcggtg
     3121 tactccggca tcggcggctg gttcggcctg cccgtcgccc tggatctgcc gaaaggcgac
     3181 ccgcgggccg cgatgggcgc ggcgggtgcg gcctatgatt ccggcctgcc cgagagcacc
     3241 acgccgggcg gcgagctggt cgtcttcggc ctcgaggccg aaaacctcta ttttcaggga
     3301 catcatcacc atcaccatca ccactgacag ctatgaccat gattacgcca agcttgcatg
     3361 cctgcaggtc gactctagag gatccccggg taccgagctc gaattcactg gccgtcgttt
     3421 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3481 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3541 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     3601 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     3661 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt atgctcccgg
     3721 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     3781 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     3841 atgtcatgat aataatggtt tcttagcacc ctttctcggt ccttcaacgt tcctgacaac
     3901 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     3961 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     4021 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     4081 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     4141 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     4201 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     4261 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     4321 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     4381 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     4441 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     4501 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     4561 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     4621 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     4681 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     4741 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     4801 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     4861 gccactgtcg actacgccat catggcgaca gcgcctttcc tttgggttct ctatatcggg
     4921 cggatcgtgg ccggcatcac cggggcgact ggggcggtag ccggcgctta tattgccgat
     4981 atcactgatg gcgatgagcg cgcgcggcac ttcggcttca tgagcgcctg tttcgggttc
     5041 gggatggtcg cgggacctgt gctcggtggg ctgatgggcg gtttctcccc ccacgctccg
     5101 ttcttcgccg cggcagcctt gaacggcctc aatttcctga cgggctgttt ccttttgccg
     5161 gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg ctctcaaccc gctcgcttcg
     5221 ttccggtggg cccggggcat gaccgtcgtc gccgccctga tggcggtctt cttcatcatg
     5281 caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt tcggcgagga tcgctttcac
     5341 tgggacgcga ccacgatcgg catttcgctt gccgcatttg gcattctgca ttcactcgcc
     5401 caggcaatga tcaccggccc tgtagccgcc cggctcggcg aaaggcgggc actcatgctc
     5461 ggaatgattg ccgacggcac aggctacatc ctgcttgcct tcgcgacacg gggatggatg
     5521 gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg gaatgccggc gctgcaagca
     5581 atgttgtcca ggcaggtgga tgaggaacgt caggggcagc tgcaaggctc actggcggcg
     5641 ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca cggcgatcta tgcggcttct
     5701 ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg ccctctactt gctctgcctg
     5761 ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac gagccgatcg ctgatcgtgg
     5821 aaacgatagg cctatgccat gcgggtcaag gcgacttccg gcaagctata cgcgccctag
     5881 aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag
     5941 cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa
     6001 gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc
     6061 accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag
     6121 gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg
     6181 cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc
     6241 cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg
     6301 ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg
     6361 ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg
     6421 tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg
     6481 taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg
     6541 atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg
     6601 tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg
     6661 aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg
     6721 gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg
     6781 aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc
     6841 ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc
     6901 atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga
     6961 cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg
     7021 ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt
     7081 tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac
     7141 cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct
     7201 ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg
     7261 cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc
     7321 gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagctcc
     7381 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
     7441 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
     7501 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
     7561 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
     7621 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
     7681 cgtgattttg tagccctggc cgacggccag caggtaggcc tacaggctca tgccggccgc
     7741 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
     7801 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
     7861 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
     7921 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
     7981 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
     8041 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
     8101 tgcagcggaa aagatccgtc gaccctttcc gacgctcacc gggctggttg ccctcgccgc
     8161 tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc
     8221 gagacaccgc ggccgccggc gttgtggata cctcgcggaa aacttggccc tcactgacag
     8281 atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt tgacagatga
     8341 ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa
     8401 cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga taagtgccct
     8461 gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat ccttgacact
     8521 tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg ggctgtccac
     8581 aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc gctaacctgt
     8641 cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg gctgcgccct
     8701 gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc tcccggcccg
     8761 ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc
     8821 caaaaatggc agccaagctg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8881 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8941 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9001 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9061 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9121 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9181 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9241 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9301 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9361 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9421 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9481 tcgtgtctta ccgggttg
//
