LOCUS       sequence-317778-        9517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2590
FEATURES             Location/Qualifiers
     source          1..9517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2455..3043
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2508..2528)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2926..2937)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3115..3348
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3426..3547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3463..3482)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3517..3536
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3587..3722
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3742..3761)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3743..3761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3771..3788)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3771..3787)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3780..3802)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3929..4384
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4016..4035)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4226..4247
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4410..4514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4515..4583,4584..5375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4733..4752)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5546..6134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6035..6054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6288..6305
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6422..6443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6458..6463,6464..6481,6482..6488)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6496..6512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6501..6523
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6520..6536
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6520..6536
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6555..6575
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6557..6575
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6603..6829
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6830..7010
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7057..7182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7675..7908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8093..8137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8368..8578
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8390..8586
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8437..8572
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8618..9220
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8618..8637)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9114..9134
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9263..9380
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtt
     1201 ttggacgttt aaagaatggt tctggttgga aagattctgg cttcctccaa caataaagtg
     1261 gtcagatctt gaggatcacg atggactcgt ctttgtaaaa ccttctcatt tatacgtgac
     1321 aattccatat gcttttctct tgctgattat cagacgtgta tttgaaaaat ttgttgcttc
     1381 acctctagca aaatcatttg gcattaaaga gacagttcga aaggttacac caaatactgt
     1441 cttagagaat tttttcaaac attccacaag gcaaccattg caaactgata tttatggact
     1501 ggcaaagaag tgtaacttga cggagcgcca ggtggaaaga tggtttagga gtcggcggaa
     1561 tcaagagagg ccttccaggc tgaagaaatt ccaggaagct tgctggagat ttgcatttta
     1621 cttaatgatc actgttgctg gaattgcgtt tctttatgat aaaccttggc tatatgactt
     1681 atgggaggtt tggaatggct atcccaaaca gcccctgctg ccatcccagt actggtacta
     1741 cattttagaa atgagttttt attggtctct gttatttaga cttggctttg atgtcaagag
     1801 aaaggatttt ctagctcata tcatccacca cctggctgct attagtctga tgagcttctc
     1861 ttggtgtgct aattatattc gcagtgggac cctcgtgatg attgtacacg atgtggctga
     1921 catttggctg gagtctgcta agatgttttc ttatgctgga tggacgcaga cctgtaacac
     1981 cctgtttttc atcttctcca ccatattttt catcagccgc ctcattgttt ttcctttctg
     2041 gattttatat tgcacgctga tcttgcctat gtatcacctc gagcctttct tttcatacat
     2101 cttcctcaac ctacagctca tgatcttgca ggtccttcac ctttactggg gttattacat
     2161 cttgaagatg ctcaacagat gtatattcat gaagagcatc caggatgtga ggagtgatga
     2221 cgaggattat gaagaggaag aggaagagga agaagaagag gctaccaaag gcaaagagat
     2281 ggattgttta aagaacggcc tcagggctga gaggcacctc attcccaatg gccagcatgg
     2341 ccattagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     2401 ttgtggaaag gacgacggca tgttcactcc tgactataga cgcgttaagt cgacaatcaa
     2461 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2521 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2581 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2641 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2701 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     2761 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2821 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     2881 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     2941 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3001 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     3061 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3121 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     3181 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3241 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3301 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     3361 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3421 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3481 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3541 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     3601 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3661 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3721 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     3781 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     3841 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3901 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     3961 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4021 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4081 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4141 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4201 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4261 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4321 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4381 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4441 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4501 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4561 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4621 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4681 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4741 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4801 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4861 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4921 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4981 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5041 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5101 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5161 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5221 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5281 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5341 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5401 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5461 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5521 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5581 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5641 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5701 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5761 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5821 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5881 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5941 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6001 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6061 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6121 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6181 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6241 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6301 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6361 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6421 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6481 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6541 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     6601 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     6661 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     6721 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     6781 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     6841 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6961 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7021 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7501 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     7801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     7861 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     7981 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8281 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     8341 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     8401 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8461 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8521 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8581 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     8641 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     8701 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     8761 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     8821 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     8881 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     8941 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     9001 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     9061 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     9121 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     9181 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
     9241 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9301 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9361 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     9421 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
     9481 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
