LOCUS       sequence-317779-        6619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses heterlogous ribosomal proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Kolber NS, Fattal R, Bratulic S, Carver GD, Badran AH
  TITLE     Orthogonal translation enables heterologous ribosome engineering in 
            E. coli.
  JOURNAL   Nat Commun. 2021 Jan 26;12(1):599. doi: 10.1038/s41467-020-20759-z.
  PUBMED    33500394
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-26"; volume: "12(1):599. doi"; pages: " 
            10.1038/s41467-020-20759-z"
COMMENT     Sequence Label: pAB184f2
FEATURES             Location/Qualifiers
     source          1..6619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      complement(3112..3143)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(3159..3818)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(3933..4883)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4931..5153
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(5715..6593)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttacctgc
      301 aggtgcagtc tcataaacag gcgcagccat tgggctgcaa ggagatagac aacgtggcaa
      361 atcatgtatc cgcactgaaa cgtgcccgcc aggatcaaaa gaaacgtatt cagaatcgtg
      421 cccagaagtc ggctatgcgt actgccgtca agcaggtgct ggtggctgta gaggcaggag
      481 acaagcaggc tgcatccgat gcgctgaagc aggcgacgtc cttgcttgac cgtgccggcc
      541 gcaagaacca gatgcatccg cgtcaggctt cccgccgtgt ttcccgcctg aatgcccatg
      601 ttaaggctat tggctaatat acctaatttt tcggaggtaa caaatatggc aactgtgatt
      661 cgcctgagcc gtggtggacg caagaagcgt cctttttatt ctgtagtcgt catggacggc
      721 cgcaagcgtc gtgatggcgc atttatcgag aagctcggtt attttgatcc gtgcaaagat
      781 cctgaagtga tccagctgga ccttgagcgt gtgaaagcat ggaccgatca gggtgcccag
      841 gtatccgatc gcgtggctag cctgatcgcc aaggcatccg cgtaatcagc ttgagtcatt
      901 aggatggtac accatgagtg aagccgagaa gctcgacacg atacagaaag cagggggtga
      961 ggcggatgtt gtcgataccg cttcagcaac tgcagaggca acccctgagg tcgtcgtagc
     1021 agaaaccgaa accactgcag cagcacctgc tgcagaagag ccggtcgtac aggccagtga
     1081 agagtcagat gagactcctg ctgaagaggc cgttagcgaa gaggactctt tcaggctgct
     1141 gtacgaggct tccctgaagg agaaaggcga gattcgcaag ggtgagatgg tcaccggcat
     1201 ggtggtgggc ctgaatgccg atgttgtcgt cattgacgta gggaccaagg ccgaaggcac
     1261 gattccgctg gctgaattcg agcaggccgg gctgccggtt ccggcaatcg gcacagaaat
     1321 caaggcattg atccagtcgg ttggcggctc ttcgggtgtg agcttgtccg tgctggccat
     1381 cagacagcgc gaggcatggg atgccattga agcggctgtg gccgatgaga gtacggtgga
     1441 tgcggtcatc acatcggaag tcaagggcgg ttaccgggtc agcctgagcg ggttgaatgc
     1501 atttatgccg cgctccgaag cggatacgga tatccatgtc ctgccggcta atctgattgg
     1561 taaggcatgc aaggtggctg tactggaagc gcggcgcagg ccggaaaata ttgttgtatc
     1621 acgcaggcgt ccgttgactg ccgctctgga agtcgagcgc gcggccttct tcgcacagca
     1681 tgcggtgggt gacaggctga gcggtgaagt gaagcggctg gctgatttcg gtgcttttgt
     1741 cgatatcggt ggtgtggatg cattgctgca cgtatccgat gtgtcgtggc gccggatcaa
     1801 gcacccgtcg gagatgctca gcgtcggtca gagcgtgagt gttgagatcg tcaagttgaa
     1861 tgcggaaacc gggaaagtgt cggtgtctat gaaggcactg cagtcggatc cgtgggataa
     1921 tgtagaagca atctatgagc cgggcatggg gctgaccggc accgtgcgcc gcctgctcga
     1981 tttcggtgct gttgtggagc tggagcccgg cattgagggt atgattcacc gttctgagct
     2041 gagctggaca aatcgtgatg tgaatccggc ggctgtgctg gctgaaggtg atgtggtgga
     2101 tgtggtggtg ctggaagttg atgcatcggc ccgtcgtatt cgtctgagcc tgaaggctgt
     2161 cagcgaaaat ccgtggcagc agtggatggc agatcatccg attggcacgc atgtcagtgg
     2221 caagatcaag aacatcactg acttcggttt ctttgtgcat gtggctgatg gtatggatgg
     2281 cctggtgcat atggagaacc tctcctggga gaagtccgga gcggctgcta tggagcctta
     2341 cagcaaggga caggaagtcg aagcggttgt gttgggcgtg gatgttgaaa aacagcgcat
     2401 cgcgctgggc atcaagcagc tctcggccga tccgtttgaa ctgtttctgg ccggcgtcaa
     2461 gcgtggcaat agcgttaatg gtaccgtggt cgatgtgaaa cctgccggcg tggtcgtgga
     2521 gttggccgat ggcgtacagg cattcatgcc gatgcgtgaa gtcccgcgcg atcacggcga
     2581 attgaaagtc ggttccgaag tggaagcgaa gattgtcgaa gtgaataaca agcgtcgtca
     2641 ggttacgctc tccattcgtc agcatctgat tcaggaagag cgggaggcta tgcgcaacta
     2701 ttcccgtcag accgctgagg aatccgctcc gtcggcactg gcgctggagt tgcagcgtaa
     2761 actctttggc aagaagtaag ccgatataaa taagaggaag aacgttcatg tctttggcgc
     2821 tggaagaaaa aaacacaatc attgaaaatt ttcgtcgtaa tgaaggcgat accggttcac
     2881 cggaagtgca ggttgccctg ttgacggagc gtatcaatca gctcggcggt cacttcaagg
     2941 acaaccacaa ggaccatcac tcacgccgcg gcctgcttaa aatggtcggt cagcgccgta
     3001 acctgcttaa atatttgaag aaagaagatt ttagtcgtta tcgtaccctg atcgaaagtc
     3061 tgggactgcg tcgctaaaca gtttcactta attaacggca ctcctcagcc aagtcaaaag
     3121 cctccggtcg gaggcttttg actacatgcc catggcgttt acgctccgcc ctgccactca
     3181 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     3241 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     3301 atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     3361 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     3421 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     3481 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     3541 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
     3601 aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     3661 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     3721 taggtacatt gagtaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     3781 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     3841 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     3901 gaacctctta cgtgccaggc caaataggcc gttcagatcc ttccgtattt agccagtatg
     3961 ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatgc
     4021 ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt
     4081 aaagcatcgt gtagtgtttt tcttagtccg ttacgtaggt aggaatctga tgtaatggtt
     4141 gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc
     4201 atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca
     4261 accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat
     4321 tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca
     4381 tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac
     4441 cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat
     4501 tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct
     4561 aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatcttaaa gcctttaacc
     4621 aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca
     4681 ccataagcat tttccctact gatgttcatc atctgagcgt attggttata agtgaacgat
     4741 accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat
     4801 aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct
     4861 ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac
     4921 caattgagat gggctagtca atgataatta ctagtccttt tcctttgagt tgtgggtatc
     4981 tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat
     5041 tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata
     5101 aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt
     5161 tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac
     5221 agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa atcgctgaat
     5281 attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt
     5341 cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg
     5401 gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct tctcagggcg
     5461 ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc
     5521 ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta
     5581 atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg tcaagaggac
     5641 atccggtcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttgctgagga
     5701 gacttaggga ccctttatga caacttgacg gctacatcat tcactttttc ttcacaaccg
     5761 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt
     5821 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa
     5881 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac
     5941 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg
     6001 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc
     6061 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat
     6121 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta
     6181 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac
     6241 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga
     6301 aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc
     6361 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt
     6421 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt
     6481 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga
     6541 tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc
     6601 atactcccac cattcagag
//
