LOCUS       sequence-31778-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector with TRE promoter followed by multiple cloning site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_Tmcs
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             197..502
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRIVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     381..400
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(531..630)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(742..761)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cccgaactag
       61 tcgactggat ccggtaccga attcgcttac taaaagccag ataacagtat gcgtatttgc
      121 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
      181 gaggtgtgct tctagaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
      241 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tagtgatccc
      301 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
      361 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
      421 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
      481 cctgatgttc tggggaatat agaattcgcg gccgcactcg agatatctag acccagcttt
      541 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
      601 cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc
      661 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
      721 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
      781 ggccgcgatt aaattccaac atgg
//
