LOCUS       sequence-317782-        6466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses heterlogous ribosomal proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Kolber NS, Fattal R, Bratulic S, Carver GD, Badran AH
  TITLE     Orthogonal translation enables heterologous ribosome engineering in 
            E. coli.
  JOURNAL   Nat Commun. 2021 Jan 26;12(1):599. doi: 10.1038/s41467-020-20759-z.
  PUBMED    33500394
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-26"; volume: "12(1):599. doi"; pages: " 
            10.1038/s41467-020-20759-z"
COMMENT     Sequence Label: pAB184g
FEATURES             Location/Qualifiers
     source          1..6466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      complement(2959..2990)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(3006..3665)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(3780..4730)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4778..5000
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(5562..6440)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttacctgc
      301 aggtgcagtg gacaggataa ggaaccgtca tggctaactc cgcacaagca cgcaagcgcg
      361 cccgccaggc cgcgaaggca aactcgcaca actcggcgct gcgctcgaaa ttccgtaccg
      421 cgatcaagtc ggttcgcaag gctgttgaag ccggcgacca ggcaaaggct gccgagctgt
      481 tcaaggctgc cgtgaagacg atcgacacga tcgccgacaa gaagatcgtt cacaagaaca
      541 aggccgctcg cagcaagagc cgcctggccg cagccgtcaa gggcctgcag gcagcagcgt
      601 aactccggtg gttttcccga aggacatcat atggttatca tccgtctggc tcgtggcggc
      661 tcgaagaagc gcccgttcta caacatcgtt gctaccgatt cgcgcaaccg tcgtgacggc
      721 cgcttcatcg agcgcgtcgg cttctacaac ccggtcgcca cgaagggcga atcgctgcgt
      781 atcgctcagg atcgcctgac gtactggcaa ggcgttggcg cgcaactgtc gccgaccgtc
      841 cagcgtctcg tgaaggaagc gcaaaaggcg caaccggctg cctaacatgg cacccatgca
      901 caatcagatt tttatgtccg acctgcaaac ctccaccccg aataccgaat cctttgcggc
      961 tctgttcgaa gagtcgctga cccgccaaga catgcgcgcc ggcgaagtga tttccgccga
     1021 agtcgtgcgc gtcgaccaca acttcgtggt cgtcaatgca ggcctgaagt ccgaggctta
     1081 cattccgatc gaggaattcc tgaacgatca gggcgaggtt gaggtgcagt cgggcgattt
     1141 cgtgtccgtc gcgatcgacg cactcgaaaa cggctatggc gacacgatcc tgtcgcgcga
     1201 caaggcgaag cgccttgcat cgtggctgtc gctggaaaag gcgctcgaca acaacgagct
     1261 cgtcaccggc acgatcaccg gcaaggtgaa gggcggcatg accgtgatgg tcaacggcat
     1321 ccgcgcgttc ctgccgggtt cgctggtcga cacgcgtccg gtcaaggaca cgaccccgta
     1381 cgaaggcaag acgctcgagt tccgcgtgat caagctcgat cgcaagcgta acaacgtcgt
     1441 gctgtcgcgt cgtgcagtga tcgaagcaac gcaaggcgaa gagcgcgcga agctgctcga
     1501 gacgctgaag gaaggcgcga tcgtcaacgg cgtggtcaag aacatcaccg actacggcgc
     1561 gttcgtcgac ctcggcggta tcgacggcct gctgcacatc accgacatcg catggcgtcg
     1621 tgtgcgtcac ccgagcgaag tcctgtcggt tggccaggaa gtcaccgcga agatcctcaa
     1681 gttcgaccaa gagaagaacc gcgtctcgct gggcatcaag caactgggcg acgatccgtg
     1741 ggaaggcatc tcgcgccgtt acccgtcggg cacgcgcctg ttcggcaagg tcacgaacat
     1801 caccgactac ggcgcattcg tcgaagtgga atcgggcatc gaaggcctcg tccacgtgtc
     1861 ggaaatggac tggaccaaca agaacgttgc gccgtcgaag gttgtccagc tgggcgacga
     1921 agtcgaagtc atggtcctcg agatcgacga agaccgtcgt cgtatcagcc tcggcatgaa
     1981 gcagtgcaag ccgaatccgt gggatgactt cagccgcaac ttcaagaagg gcgacaagat
     2041 cacgggcgca atcaagtcga tcaccgactt cggcgtgttc atcggtctgc cgggcggcat
     2101 cgacggcttg gtccacctgt cggacctgtc gtggagcgaa gccggcgaag aagccgttcg
     2161 caagtacaag aagggcgacg aagtcgaagc aatcgtgctc ggtatcgacg tcgagaagga
     2221 gcgcatttcg ctcggcatca agcagctcga aggcgacccg ttcagcaact acgttgcaat
     2281 gaacgacaag ggctcgatcg tcgacggcgt cgtgaagacg gtcgatgcga agggtgcggt
     2341 cgtcacgctg acgggcgaca tcgaaggcta cctgcgtgcg tcggaaatct cgcaggatcg
     2401 cgtcgaagat gcacgcaacg tgctgaagga aggcgacaag gtcaacgcga tggtgatcaa
     2461 catcgatcgc aagtcgcgcg gcatcaacct gtcgatcaag gcgaaggatt cggccgaaca
     2521 acaggaagcg atccgcggcc tgcagtcgga ctccagcgct gctgcgaccg gtacgaccaa
     2581 cctcggcgcg ctgctgaagg cgaagctcga cggccagaac cagtaagcct tacgtcagtt
     2641 agaaaggaaa tcacatgtct gttgcagata tcaagaagtc ggaagtcgtt gctcagttcg
     2701 cccgcggtac caacgacacg gggtcgcccg aagtccaggt cgcactgctg accgcacgta
     2761 tcgtcgaact gacgggtcac ttcaagaccc acgcgaagga tcaccacagc cgccgcggcc
     2821 tgctgcgcat ggtgagccgc cgccgcaagc tgctcgacta cctcaagggc aaggatgccg
     2881 accgttaccg cgcactgatc gagaagctgg gtctgcgtaa gtaatcgagg cgacttaatt
     2941 aacggcactc ctcagccaag tcaaaagcct ccggtcggag gcttttgact acatgcccat
     3001 ggcgtttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     3061 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     3121 ttgtcgcctt gcgtataata tttgcccata gtgaaaacgg gggcgaagaa gttgtccata
     3181 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     3241 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     3301 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     3361 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     3421 agctcaccgt ctttcattgc catacggaac tccggatgag cattcatcag gcgggcaaga
     3481 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     3541 gtaatatcca gctgaacggt ctggttatag gtacattgag taactgactg aaatgcctca
     3601 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     3661 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     3721 gatcttattt cattatggtg aaagttggaa cctcttacgt gccaggccaa ataggccgtt
     3781 cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc
     3841 catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa ttttgcctca
     3901 aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta
     3961 cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg
     4021 ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac
     4081 gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa
     4141 tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt
     4201 tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt
     4261 ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca
     4321 aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa aagataaggc
     4381 aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc
     4441 cactggaaaa tcttaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc
     4501 agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc
     4561 tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc
     4621 gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca
     4681 tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat
     4741 tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta
     4801 gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc tggaaaactt
     4861 gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat
     4921 attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat
     4981 cccagccctg tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg
     5041 caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc
     5101 ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag
     5161 tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg
     5221 taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga
     5281 aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg
     5341 actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat
     5401 cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag
     5461 gcttacccgt cttactgtca agaggacatc cggtcaaata aaacgaaagg ctcagtcgaa
     5521 agactgggcc tttcgttttg ctgaggagac ttagggaccc tttatgacaa cttgacggct
     5581 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat
     5641 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg
     5701 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg
     5761 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc
     5821 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg
     5881 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta
     5941 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa
     6001 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc
     6061 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc
     6121 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc
     6181 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt
     6241 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg
     6301 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc
     6361 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga
     6421 tcattttgcg cttcagccat acttttcata ctcccaccat tcagag
//
