LOCUS       sequence-317785-        6406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses heterlogous ribosomal proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Kolber NS, Fattal R, Bratulic S, Carver GD, Badran AH
  TITLE     Orthogonal translation enables heterologous ribosome engineering in 
            E. coli.
  JOURNAL   Nat Commun. 2021 Jan 26;12(1):599. doi: 10.1038/s41467-020-20759-z.
  PUBMED    33500394
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-26"; volume: "12(1):599. doi"; pages: " 
            10.1038/s41467-020-20759-z"
COMMENT     Sequence Label: pAB184h
FEATURES             Location/Qualifiers
     source          1..6406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      complement(2899..2930)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2946..3605)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(3720..4670)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4718..4940
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(5502..6380)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttacctgc
      301 aggtgcagta gtttttggga gttagaccct tggcaaataa caaatctgct aagaagcgcg
      361 ctatccaagc tgagaaacgt cgccagcaca atgctagccg tcgttctatg atgcgcactt
      421 acatgaagaa aactgttgct gcaatcgctg ctggcgacaa agaagctgca actgctgcat
      481 tcgctgtagt tacaccaatc ctagaccgca tggcgactaa aggccttatt cacaagaata
      541 aagcagctcg tcacaagtct cgcttctttg ctgctatcaa cgctctgtaa tagagttttg
      601 aaatgcaaag aggacgacat ggtaaccatt cgtttggcac gtcacggcgc gaaaaagcgt
      661 ccattctatc aaatcgttgt agctgacagc cgcaactcag caactggccg tttcatcgag
      721 aaagtaggtt tctttaaccc tactgctact ggtcaagaag aaggcctgcg tctagaccta
      781 gatcgcgtga accactgggt ttcacaaggc gcatctctgt ctgatcgcgt agcacagcta
      841 gttaaaaccg ctcaaaaagc ggcttaatta ttactaatta ttgaagatta aataaatgac
      901 tgaatctttt gctcaactct ttgaagagtt tctgaacgag acccaattcc aacaaggtac
      961 tatcgttaaa ggtactgtag tagcaatcga gaacggctac gttctggttg atgctggcct
     1021 gaagtctgag tctgctatcc ctgctgaaca attcaagaac gctgctggcg aactagaagt
     1081 tcaagtcggt tctcaagtag acgtagctct ggacgcggtt gaagatggtt tcggcgaaac
     1141 tcaactttct cgtgagaaag cgaagcgcca tgaagcttgg atcgtacttg aaaaagctta
     1201 cgaagaagct gcaactgtta tcggtatcat taacggtaaa gttaaaggtg gtttcactgt
     1261 tgaactgaac ggtatccgtg cgttcctgcc aggttctctg gttgacgtac gtccaatccg
     1321 tgacactgct cacttagaaa acaaagagct agagttcaaa gttatcaagc tagaccagaa
     1381 gcgtaacaac gttgttgttt ctcgtcgtgc agttatcgaa tctgaaagca gcgtagagcg
     1441 tgatgaactg cttgaaactc tgcaagaagg tactgaagtt aaaggtatcg ttaagaacct
     1501 gactgactac ggtgcgttcg ttgatctggg cggcgttgac ggtctgctac acatcactga
     1561 catggcttgg aagcgcgtta agcatccatc agaaatcgtt aacgttggtg acgagatcct
     1621 agttaaagta ctgaagttcg atcgtgatcg tactcgcgta tctctaggtc tgaaacagct
     1681 aggcgaagat ccatgggtag caatcgctaa gcgttaccca gaaggtcaca aactgtctgg
     1741 ccgtgtaact aacctgactg actacggctg cttcgttgaa atcgaagaag gcgttgaagg
     1801 tctagtacac gtttctgaaa tggactggac gaacaagaac atccacccat ctaaagttgt
     1861 taatgttggc gacgaagttg aggttatggt tctggatatc gacgaagagc gtcgtcgtat
     1921 ctctctaggc ctgaaacagt gtaaagctaa cccatggcaa tcattcgctg aagcgcaagc
     1981 taaaggcgac aaagtaactg gtaagatcaa gtctatcact gactttggta tcttcatcgg
     2041 tctagaaggc ggtatcgacg gtctggttca cctgtctgac atctcttgga acgtaccagg
     2101 agaagaagct gttcgtgaat tcaagaaagg cgacgaaatc tctgcagttg ttctagcagt
     2161 agacgctgag cgtgagcgta tttctctggg catcaagcaa atggaaaacg atccattcaa
     2221 cgcttatgtt tcagacaaca agaaaggtgc tctggttaac ggtactgtaa ctgcagttga
     2281 cgcgaaaggc gcaaccatcg agctagaaga cggcgttgaa ggttacatcc gcgcttctga
     2341 agtatcacgt gaccgtatcg aagatgcatc actgatcctg aacgttggtg acaaagttga
     2401 agcgaaattt actggtgttg accgtaaaaa ccgcgtaatc aacctgtcta tcaaagcgaa
     2461 agatgaagct gaagagcaag aagtaatggc aactctgaac aagcaagatg aagctgcgtt
     2521 cggtaatgct atggctgacg ctttcaaagc agcgaaaggc gaataatagc caaaaaactc
     2581 tttaggagag aattatgtct ctgaatgcag aaactaaagc agcaatcgtt gctgaatatg
     2641 cacgttgcga aaatgacaca ggttcaccag aagtacagat cgctctactg actgcttcta
     2701 tcaaccacct gcaaggtcac ttccaagcac acaaaggcga tcaccacagc cgtcgtggtc
     2761 tgctacgtat ggtttcaagc cgtcgtaaac tgctggacta cctgaaaggt aaagatctgt
     2821 ctcgttacca agatctgatc aaacgtctag gcctgcgtcg ttaattcgtc gcacttaatt
     2881 aacggcactc ctcagccaag tcaaaagcct ccggtcggag gcttttgact acatgcccat
     2941 ggcgtttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     3001 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     3061 ttgtcgcctt gcgtataata tttgcccata gtgaaaacgg gggcgaagaa gttgtccata
     3121 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     3181 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     3241 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     3301 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     3361 agctcaccgt ctttcattgc catacggaac tccggatgag cattcatcag gcgggcaaga
     3421 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     3481 gtaatatcca gctgaacggt ctggttatag gtacattgag taactgactg aaatgcctca
     3541 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     3601 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     3661 gatcttattt cattatggtg aaagttggaa cctcttacgt gccaggccaa ataggccgtt
     3721 cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc
     3781 catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa ttttgcctca
     3841 aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta
     3901 cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg
     3961 ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac
     4021 gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa
     4081 tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt
     4141 tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt
     4201 ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca
     4261 aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa aagataaggc
     4321 aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc
     4381 cactggaaaa tcttaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc
     4441 agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc
     4501 tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc
     4561 gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca
     4621 tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat
     4681 tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta
     4741 gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc tggaaaactt
     4801 gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat
     4861 attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat
     4921 cccagccctg tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg
     4981 caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc
     5041 ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag
     5101 tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg
     5161 taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga
     5221 aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg
     5281 actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat
     5341 cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag
     5401 gcttacccgt cttactgtca agaggacatc cggtcaaata aaacgaaagg ctcagtcgaa
     5461 agactgggcc tttcgttttg ctgaggagac ttagggaccc tttatgacaa cttgacggct
     5521 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat
     5581 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg
     5641 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg
     5701 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc
     5761 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg
     5821 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta
     5881 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa
     5941 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc
     6001 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc
     6061 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc
     6121 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt
     6181 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg
     6241 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc
     6301 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga
     6361 tcattttgcg cttcagccat acttttcata ctcccaccat tcagag
//
