LOCUS       sequence-317810-        9555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018379
FEATURES             Location/Qualifiers
     source          1..9555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             407..429
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(1492..1503)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2341..2361
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2362..2385
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2411..2467
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2468..2485)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2468..2484)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2477..2499)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2693..2712)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2812..2834
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2872..2890)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3676..4875
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(5106..6254)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6526..6897)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6930..7039
                     /note="incP origin of transfer"
     rep_origin      7192..7823
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8286..8874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8775..8794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9028..9045
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9162..9183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9198..9203,9204..9221,9222..9228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9236..9252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9241..9263
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9260..9276
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9260..9276
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggcacttcgg atgacttccg agcgagcctg gaacctcaga aaaacgtctg agagataccg
       61 cggatctcac acaggaaatt tcgcaacgca aaatgctgaa tttagcaggc aagtttcctg
      121 ataaagggcc ctgtgagtgc gacattcgac gcgcgaaagc gagttgacgg cgcccgaaat
      181 tttcttagag ttcgttcaag cttcggtttc ggggagccgc taagggatgc ggacccagcc
      241 gggtaagtcg ccgggaacta tggaataatc ctaatttctg tgcggcaggc gcgagctgcc
      301 ggagcgaata tcatccctgt cgcaaacagc aggtcaatca acggtgggta gcgggctgct
      361 atccagcggc ggataagact tgtcggagga atcctctaga aataattttg tttaacttta
      421 agaaggagat ataccatggg caagccgctc agcggcagca ccatggccgg catgatcgct
      481 ggcgcgctga tgctggccat cgcctccggg tccgccctcg ccaacgagga gatcgtgcga
      541 gcatcgaaga atcccgacct gtggcccgcc ccgggccgcg acctggccat gacgcggcac
      601 tccccgctga aggatatcaa cacggccaat gtaggcaagt tgcagatgat gtggagccag
      661 tcgaccggcg ccctccgcgg ccaccaggga caaccgatcg tcgtggaggt ggccggcacg
      721 ccgatgatgt tcttcgtgag cccgtggccg aacatcgtcc aggccctcga cctgagcgac
      781 ccggaccacc cgaagcaggt ctggaactat aagaagaaaa ccgaccgcga cgagctggcc
      841 gtcacgcgcg cctgctgcga caccatcaac cgcggactga actacgcgga cggcaaggtg
      901 gtcttccaca ccctggacgg ctacgtcatc gcgctcgatg ccaagacggg caaagagatc
      961 tggacggtca agcacgcctg gtctgacaag ggcgagacga tcaccggccc gacgctgatc
     1021 gccgagaaca aggtgatcat tggcttcggc ggcgacgagt tcgccgcgcg gggccgcgtg
     1081 acggcgtacg acctcaagac cggcaagcag gtctggaact gtcactcgac cggctccgac
     1141 aaggacgtat gccttacgcc cgagaccaac aaagccaacc cgcactacgg tacgtacggg
     1201 aagaatctcg ggatgatcac gtacccgaac gacgaatgga aaatcggcgg cggcgcgccc
     1261 tgggagtggt tcacctacga cccggagctt cggcttgtgt acgtgcccac cggcaacccg
     1321 gggcattggt cgccgtccta ccgctgcggc gagaccgggg ccaatctgac ccacgagaaa
     1381 tgcaaccagt acgatcccga gctccggtcc ggcaggtggg acaacaagtg ggcgatgacg
     1441 atcttcgcgc ggaagatcga taccggcgaa gccgtgtggg cgtaccaaat gacgaccttc
     1501 gatcagtggg attacgatgg ggtcaatgag ccggtgctcg tggacatgaa ggtggatggc
     1561 aagatgcgga agaccctcgt gcactttgac cgcaacggct tcgcctacgt cctggaccgc
     1621 gccgacggca ccctcctgcg cgcccacaag ttctttccgc tgaactgggc ggagaaagtc
     1681 gacctcaaga ccggccgccc ggtcaagatc aaggagcatt ccccattcgc ccgccatgtt
     1741 tcgacccagg tctgtccgtc gagccttggg gcgaagaacc agcagcctat tgccatcgac
     1801 cccaaggagc cccacatcgc gtacgtggcg acgaactggt ggtgcatgga gtacgagccg
     1861 caggagcgga cccataccca gcagggaatg ctctacgtgt tcgcgaatgt gttcacctac
     1921 ccgatcgaga agggcgtggc gtcgaaggtg cagaagttca acgtcctcac tggcgaaacc
     1981 gagtgggcga tcccggacgc ctatccggac tgggggggca tgctgaccac cgacggcgac
     2041 ctcctgttct atggctccct cagcggggat ttccgcgccg tcgatcgcaa gtcgggcaaa
     2101 gtcctgtggt cccgcaagct gggcagcggg atcatcggaa acccgatcac ctacaagatc
     2161 aagggcaagc agtacgtctc cgtcctggcc gggatcggcg gctggatcgg cctgccggtg
     2221 accgccgaat tggacttcga cgacaagtac ggcgccatcg gggcgaccgc gatggcgaag
     2281 ctgaccggtc tcgacaagat cccgcaaggc ggcgtgcttt acaccttccg tatcgacgcc
     2341 gaaaacctct attttcaggg acatcatcac catcaccatc accactgaca gctatgacca
     2401 tgattacgcc aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct
     2461 cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2521 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2581 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     2641 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     2701 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     2761 gacgggcttg tatgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2821 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     2881 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagcac cctttctcgg
     2941 tccttcaacg ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag
     3001 tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa
     3061 cagcggcgag agcggagcct gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc
     3121 ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg attgtcatca gcgcattgac
     3181 ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc
     3241 catctgcggt gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag
     3301 cagcgcctgc ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc
     3361 tctcggcacc gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag
     3421 cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc tgaaccccca accgttccgc
     3481 cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca gggcggcacg
     3541 gatcactgta ttcggctgca actttgtcat gcttgacact ttatcactga taaacataat
     3601 atgtccacca acttatcagt gataaagaat ccgcgcgttc aatcggacca gcggaggctg
     3661 gtccggaggc cagacgtgaa acccaacata cccctgatcg taattctgag cactgtcgcg
     3721 ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg
     3781 gttcactcga acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttggtg
     3841 caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccaatc
     3901 ttgctcgtct cgctggccgg cgccactgtc gactacgcca tcatggcgac agcgcctttc
     3961 ctttgggttc tctatatcgg gcggatcgtg gccggcatca ccggggcgac tggggcggta
     4021 gccggcgctt atattgccga tatcactgat ggcgatgagc gcgcgcggca cttcggcttc
     4081 atgagcgcct gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg gctgatgggc
     4141 ggtttctccc cccacgctcc gttcttcgcc gcggcagcct tgaacggcct caatttcctg
     4201 acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt acgccgggag
     4261 gctctcaacc cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt cgccgccctg
     4321 atggcggtct tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt
     4381 ttcggcgagg atcgctttca ctgggacgcg accacgatcg gcatttcgct tgccgcattt
     4441 ggcattctgc attcactcgc ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc
     4501 gaaaggcggg cactcatgct cggaatgatt gccgacggca caggctacat cctgcttgcc
     4561 ttcgcgacac ggggatggat ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc
     4621 ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag
     4681 ctgcaaggct cactggcggc gctcaccagc ctgacctcga tcgtcggacc cctcctcttc
     4741 acggcgatct atgcggcttc tataacaacg tggaacgggt gggcatggat tgcaggcgct
     4801 gccctctact tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa
     4861 cgagccgatc gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc
     4921 ggcaagctat acgcgcccta gaattgtcaa ttttaatcct ctgtttatcg gcagttcgta
     4981 gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc agtgcccgct
     5041 tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac tgaccccaca
     5101 aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg
     5161 ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg
     5221 gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc
     5281 gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat
     5341 atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc
     5401 tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc
     5461 gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac
     5521 aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa
     5581 agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac
     5641 acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc
     5701 ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc
     5761 ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg
     5821 tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg
     5881 tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg
     5941 gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa
     6001 cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg
     6061 gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc
     6121 gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt
     6181 tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca
     6241 aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc
     6301 ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca
     6361 atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg
     6421 ccctgcacga ataccagctc cgcgaagtcg ctcttcttga tggagcgcat ggggacgtgc
     6481 ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg gctctgccct
     6541 cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt cgatcttcgc
     6601 cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt cggtgagcca
     6661 gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca gctcgcggac
     6721 gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca gcaggtaggc
     6781 ctacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag
     6841 gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat cagccatccg
     6901 cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt
     6961 gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc
     7021 tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac
     7081 cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat
     7141 aatgaccccg aagcagggtt atgcagcgga aaagatccgt cgaccctttc cgacgctcac
     7201 cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga
     7261 aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgtggat acctcgcgga
     7321 aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc
     7381 cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc
     7441 agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac
     7501 aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg
     7561 aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt
     7621 gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt
     7681 tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt
     7741 ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc
     7801 ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct
     7861 cggccgcgaa cggcctcacc ccaaaaatgg cagccaagct gaccacttct gcgctcggcc
     7921 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7981 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8041 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8101 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8161 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8221 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8281 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8341 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8401 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8461 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8521 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8581 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8641 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8701 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8761 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8821 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8881 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8941 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9001 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9061 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9121 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9181 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9241 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctagcttcc
     9301 cgcttggtcg ggccgcttcg cgagggcccg ttgacgacaa cggtgcgatg ggtcccggcc
     9361 ccggtcaaga cgatgccaat acgttgcgac actacgcctt ggcactttta gaattgcctt
     9421 atcgtcctga taagaaatgt ccgaccagct aaagacatcg cgtccaatca aagcctagaa
     9481 aatataggcg aagggacgct aataagtctt tcataagacc gcgcaaatct aaaaatatcc
     9541 ttagattcac gatgc
//
