LOCUS       sequence-31782-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector with TRE-tight promoter followed by multiple cloning 
            site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_TTmcs
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             124..429
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRIVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     308..327
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(458..557)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(669..688)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(747..766)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtaccgaatt cgcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
       61 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgcttct
      121 agaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
      181 gtacagagtg atattattga cacgcccggg cgacggatag tgatccccct ggccagtgca
      241 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
      301 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
      361 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
      421 ggaatataga attcgcggcc gcactcgaga tatctagacc cagctttctt gtacaaagtt
      481 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      541 aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg
      601 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      661 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
      721 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
      781 aggtgcgaca atctatcgct tgtat
//
