LOCUS       sequence-31783-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector with human U6 promoter driving control Luciferase 
            miR30-based shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_hU6miR-Luc-A
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           91..212
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           240..254
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           282..408
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(447..546)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(658..677)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(736..755)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttggctttat atatcttgtg gaaaggacga aacaccgtgc tcgcttcggc agcacatata
       61 ctagtcgact agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc
      121 gttgcctgca catcttggaa acacttgctg ggattacttc ttcaggttaa cccaacagaa
      181 ggctcgagaa ggtatattgc tgttgacagt gagcgcccgc ctgaagtctc tgattaatag
      241 tgaagccaca gatgtattaa tcagagactt caggcggttg cctactgcct cggaattcaa
      301 ggggctactt taggagcaat tatcttgttt actaaaactg aataccttgc tatctctttg
      361 atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt tcaattggaa
      421 gactaatgcg ttttcgagat atctagaccc agctttcttg tacaaagttg gcattataag
      481 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      541 tatttgccat ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
      601 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
      661 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
      721 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
      781 tctatcgctt gtatgggaag cccgatgcgc caga
//
