LOCUS       sequence-31787-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector withTRE promoter driving control Luciferase miR30-based
            shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_TmiR_Luc-A
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           88..209
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           237..251
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           279..405
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(444..543)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(655..674)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(733..752)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaacta
       61 gtcgactagg gataacaggg taattgtttg aatgaggctt cagtacttta cagaatcgtt
      121 gcctgcacat cttggaaaca cttgctggga ttacttcttc aggttaaccc aacagaaggc
      181 tcgagaaggt atattgctgt tgacagtgag cgcccgcctg aagtctctga ttaatagtga
      241 agccacagat gtattaatca gagacttcag gcggttgcct actgcctcgg aattcaaggg
      301 gctactttag gagcaattat cttgtttact aaaactgaat accttgctat ctctttgata
      361 catttttaca aagctgaatt aaaatggtat aaattaaatc acttttttca attggaagac
      421 taatgcgttt tcgagatatc tagacccagc tttcttgtac aaagttggca ttataagaaa
      481 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
      541 ttgccatcca gctgcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
      601 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
      661 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
      721 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      781 atcgcttgta tgggaagccc gatgcgccag a
//
