LOCUS       sequence-31788-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector with TRE promoter driving mouse G alpha 12 miR30-based 
            shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_TmiR_G12-E
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           89..210
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           238..252
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           280..406
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(439..538)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(650..669)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(728..747)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaatct
       61 agtcgactag ggataacagg gtaattgttt gaatgaggct tcagtacttt acagaatcgt
      121 tgcctgcaca tcttggaaac acttgctggg attacttctt caggttaacc caacagaagg
      181 ctcgagaagg tatattgctg ttgacagtga gcgacgacac catcttcgac aacattagtg
      241 aagccacaga tgtaatgttg tcgaagatgg tgtcgctgcc tactgcctcg caattcaagg
      301 ggctacttta ggagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat
      361 acatttttac aaagctgaat taaaatggta taaattaaat cacttttttc aattcgcggc
      421 cgcactcgag atatctagac ccagctttct tgtacaaagt tggcattata agaaagcatt
      481 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
      541 atccagctgc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
      601 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
      661 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
      721 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
      781 ttgtatggga agcccgatgc gccaganttg tttctgaaac atggcaaagg tagcgttgcc
      841 aatgatgtta cagatgagat gg
//
