LOCUS       sequence-317897-       10344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full length mouse KIF13B for purification from insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Duellberg C, Auer A, Canigova N, Loibl K, Loose M
  TITLE     In vitro reconstitution reveals phosphoinositides as cargo-release 
            factors and activators of the ARF6 GAP ADAP1.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2010054118. doi: 
            10.1073/pnas.2010054118. Epub 2020 Dec 18.
  PUBMED    33443153
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-5"; volume: "118(1). pii: 
            2010054118. doi"; pages: " 10.1073/pnas.2010054118. Epub 2020 Dec 
            18"
COMMENT     Sequence Label: KIF13B
FEATURES             Location/Qualifiers
     source          1..10344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(589..657,658..1449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(807..826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2109..2128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2362..2379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3524..3529,3530..3546,3547..3552))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3920..3937
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4062..4079
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4101..4121
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    9834..9968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9871..9890)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9925..9944
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  9997..10162
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
     4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggga gatagcaaag
     4141 tgaaagtggc cgttagagtt agaccgatga accgtagaga gattgacctg catacaaagt
     4201 gtgtcgttga tgtggaggct aacaaggtca tcctgaaccc tgtgaacacc aacctgagca
     4261 agggagacgc tcgcggtcag cccaagatct tcgcctacga ccactgcttc tggtccatgg
     4321 acgagagcgt gcgtgaaaag tacgctggtc aggaagacgt cttcaagtgc ctgggcgaga
     4381 acatcctgca gaacgccttc gacggataca acgcttgcat cttcgcctac ggtcagaccg
     4441 gttctggcaa gtcatacact atgatgggta ccgctgacca gcctggcctg atcccaaggc
     4501 tgtgctccgg tctgttcgag agaactcaga aggaggaaaa cgaggaacag tctttcaagg
     4561 tcgaagtgtc atacatggag atctacaacg aaaaggtgcg cgacctgctg gacccaaagg
     4621 gctccaggca gaccctgaag gtcagagagc acagcgtgct gggtccatac gtcgacggcc
     4681 tgtccaagct ggctgtgact agctacaagg acatcgaatc cctgatgagc gagggtaaca
     4741 agtctcgcac tgtcgctgcc accaacatga acgaggaatc cagccgttca cacgccgtgt
     4801 tcaagatcac cctgactcac accctgtacg acgtcaagtc tggcacctca ggagaaaaag
     4861 tgggcaagct gtccctggtc gacctggctg gaagcgagag agctactaag accggtgctg
     4921 ctggcgaccg tctgaaggaa ggatcaaaca tcaacaagtc cctgaccact ctgggtctgg
     4981 tcatctccgc tctggccgac cagggagctg gcaagaacaa gaacaagttc gtgccctacc
     5041 gtgacagcgt cctgacttgg ctgctgaagg acagcctggg tggcaactct aagactgcta
     5101 tggtggccac cgtctcccca gctgccgaca actacgacga gactctgagc accctgcgtt
     5161 acgctgacag ggccaagcac atcgtgaacc acgctgtggt caacgaagac cctaacgcca
     5221 ggatcatccg cgacctgcgc gaggaagtcg agaagctgag ggaacagctg accaaggctg
     5281 aagccatgaa gtctcccgag ctgaaggaca gactggagga atcagaaaag ctgatccagg
     5341 agatgactgt gacctgggag gaaaagctga ggaagaccga ggaaatcgct caggaaagac
     5401 agaagcagct ggagtctctg ggaatctcac tgcagacttc cggcatcaag gtcggagacg
     5461 acaagtgctt cctggtcaac ctgaacgctg acccagccct gaacgaactg ctggtgtact
     5521 acctgaagga gcacaccctg atcggttccg ctaacagcca ggacatccag ctgtgcggca
     5581 tgggaatcct gcctgaacac ggtatcatcg acatcactcc agagggacag gtggtcctga
     5641 ccccacagaa gaacactcgc accttcgtga acggttcttc agtctccagc cccatccagc
     5701 tgcaccacgg agacaggatc ctgtggggta acaaccactt cttcagactg aacctgccta
     5761 agaagaagaa gaaggctgag cgcgaagacg aggaccgtga agcctctctg aagaacgact
     5821 cttcatccga gcagctggac gctgacggtg acagctcttc agaggtgtcc agcgaaatca
     5881 acttcaactt cgaatacgct cagatggagg tcaccatgaa ggccctgggc tctaacgacc
     5941 ctatgcagtc aatcctgtct tcactggaac agcagcacga ggaagagaag cgttccgccc
     6001 tggagcgcca gcgtctgatg tacgaacacg agctggaaca gctgcgccgt aggctgtctc
     6061 ccgagagaca gaactgccgc ggcgtggacc gtctgtcatt ccacagccca tctgctcagc
     6121 agcgcctgaa gcagtgggct gaagagcgtg aagccactct gaacaactcc ctgatgaggc
     6181 tgagagaaca gatcgtgaag gccaacctgc tggtcaggga ggctagctac atcgccgaag
     6241 agctggacaa gagaactgag tacaaggtga ccctgcagat ccctacttcc agcctggacg
     6301 ctaaccgcaa gcgtggttct ctgctgtcag aacccgccat ccaggtcaga cgcaagggca
     6361 agggcaagca gatctggtcc ctggagaagc tggaaaacag gctgctggac atgcgcgacc
     6421 tgtaccagga gtggaaggaa tgcgaagagg actcccctgt gtcacgctcc tacttcaagc
     6481 gtgctgaccc cttctacgac gagcaggaaa accacagcct gatcggcgtg gccaacgtct
     6541 tcctggaaac cctgttctac gacgtgaagc tgcagtacgc tgtccccatc atcaaccaga
     6601 agggtgaagt ggccggcagg ctgcacgtcg aggtcatgag actgagcgga gctatcggag
     6661 agaggatcgc cggaggtgac gacccaaccg aagtctcttc agagaaggaa gctcaggaga
     6721 acagactggt gtgcatggtc aagatcctgc aggccactgg tctgcctcag cacctgtgcc
     6781 acttcgtgtt ctgcaagtac gacttctggg accagcagga accagtcacc gtggctcctg
     6841 aggtcgacac ctccagctct cccacttcca agcagccaca gtgcatggtg gtcttcgacc
     6901 actgctccga gttcagcgtg aacatcactg aggacttcat cgaatacctg agcgagggag
     6961 ctctggccat cgaagtctac ggtcacaaga tgaacgaccc acgcaagaac cctgctctgt
     7021 gggacctggg aatcatccag gccaagactc gctccctgag ggacagatgg agcgaagtga
     7081 cccgtaagct ggagttctgg gtccagatcc tggagcagaa cgaaaacggt gactactgcc
     7141 ccgtcgaggt catcgctgcc aaggacgtgc caaccggcgg aatcttccag ctgcgtcagg
     7201 gacagtcccg tagggtccag gtggaagtca agagcgtgca ggagtctggc actctgccac
     7261 tgatggaaga gtgcatcctg tccgtcggaa tcggttgcgt gaaggtccgt cccctgagga
     7321 gcccaaagat ccacgagaac gtgcacgaag aggaagagga catggactcc taccaggaca
     7381 gggacctgga gagactgaga cgcaagtggc tgaacgctct gaccaagcgc caggaatacc
     7441 tggaccagca gctgcagaag ctggtgagca agcacgacaa gactgaggac gacgctgacc
     7501 gtgaagccca gctgctggag atgcgcctga ctctgaccga agagcgtaac gctgtgatgg
     7561 tcccttctgc cggctcagga atcccaggtg ctcctgccga atggactcct gtgcccggca
     7621 tggaaaccca catcccagtc atcttcctgg acctgaacgc tgacgacttc tcatcccagg
     7681 acaacctgga cgaccctgag gctggatggg acgctactct gaccggagaa gaggaagagg
     7741 aattcttcga actgcagatc gtgaagcagc acgacggcga agtcaaggct gaggcctcct
     7801 gggacagcgc tgtgcactca tgcccacagc tgtccaaggg aacccctgcc gacgagcgcg
     7861 tcttcctgat cctgcgtgtg actgtccagc tgtcccaccc tgctgacatg cagctggtgc
     7921 tgcgcaagcg tatctgcgtg cacgtccacg gcaggcaggg attcgcccag tctctgctga
     7981 agaagatgtc acacagaagc tctatccccg gttgcggcgt gaccttcgaa atcgtctcca
     8041 acatcccaga ggacgctcag ggtgtggagg aaagggaagc tctggccaga atggctgcca
     8101 acgtcgagaa ccccgcttct gccgactcag aagcttacat cgagaagtac ctgcgcagcg
     8161 tgctggccgt cgagaacctg ctgaccctgg acaggctgag acaggaagtg gccgtcaagg
     8221 agcagctgac tggaaagggc aagctgtccc gtaggagcat ctcatcccca tctatgaacc
     8281 gcctgagcgg ctctcgtcag gagctgtcac cttcccacag cctgggatca aacaagggtc
     8341 gctgggaaag ccagcaggac gtgtctcaga ccctggtgtc ccgtggaatc gctccaggac
     8401 ctccagctct gtctgtgtca cctcagaaca accagtctcc agaccctggc ctgggtggag
     8461 tcgctgcttc ttacctgaac cccgtgaagt cactggtccc ccagatgcca aagctgctga
     8521 agtcactgtt cccagtgagg gacgacagac gcggaagaca cagctctcct ctggctcacc
     8581 agcccgtgcc aaggatcctg gtccagccaa ctttctctga cacctgggcc actagaaccg
     8641 aggaagtgtc acaccaggtc ggcgctgccc ctctggctga ggtcatggct gcccccgctg
     8701 tcaagatctg cgacaagcca gtgaaggtgt cctccccacc ttctcctatg gtggtcaccc
     8761 agccaccaga gggtcaggac ggacctccat ctccactgtc agaggctagc tctggatact
     8821 tctcccacag cgtgtcatcc gccactctgt cagagactct gaccctgggc ctggacacca
     8881 ctggcctggg atcccagact cccggaagcc cacctgctct gtgccaggtc acccctgagc
     8941 ccgaactggc cttcctgtca tgtactcagt cccacccacc agctcctgag gaagctcacg
     9001 tgcctgctgc ccccactcag tctaccgagt tggaagtccc acgtgctcct ctgctgagcg
     9061 agccagcttc tgctgtgcca acctcaccat tccgcatccg taaggtcagg acttcagaac
     9121 tgaagtcctt caccggcatg ctgggaggtg ctagctctgg agccgaggaa gaccctctgg
     9181 ctagcgagga cccatctaac gctcgtggtc agactctggg ccgtctggaa gtgacctctg
     9241 actcagagga cgcttccgag gtcccagaat ggctgcgcga gggagaatac gtggtcgtgg
     9301 gaactaacaa gaccggtatc gtgcgttaca tcggtccaac cgacttccag gaaggaactt
     9361 ggatcggtgt cgagctggac ctgcctgctg gcaagaacga cggatctatc ggcggaaagc
     9421 agtacttccg ttgcaaccct ggttacggcc tgctggtgcg cccttcacgc gtgcgtaggg
     9481 ctgtcggaac cggtagacgc cgttcatccg gcctgcagcc tcagggagct cccgaggtca
     9541 ggagatccgc caccatctcc ggtagcgcta ctaacctggc cagcctgacc gctgccctgg
     9601 cgaaaggaga tagaagttac aagaaccccg agaacagaaa atcgtgggcg tcataagcgg
     9661 ccgctttcga atctagagcc tgcagtctcg aggcatgcgg taccaagctt gtcgagaagt
     9721 actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     9781 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     9841 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9901 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9961 tctggatctg atcactgctt gagcctagga gatccgaacc agataagtga aatctagttc
    10021 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
    10081 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc
    10141 ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac
    10201 atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat
    10261 gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt
    10321 atttgcagcc tgaatggcga atgg
//
