LOCUS       sequence-317899-        6565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Motile fragment of mouse KIF13B (aa 1-584) for purification from 
            insect cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Duellberg C, Auer A, Canigova N, Loibl K, Loose M
  TITLE     In vitro reconstitution reveals phosphoinositides as cargo-release 
            factors and activators of the ARF6 GAP ADAP1.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2010054118. doi: 
            10.1073/pnas.2010054118. Epub 2020 Dec 18.
  PUBMED    33443153
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-5"; volume: "118(1). pii: 
            2010054118. doi"; pages: " 10.1073/pnas.2010054118. Epub 2020 Dec 
            18"
COMMENT     Sequence Label: KIF13B 1-584
FEATURES             Location/Qualifiers
     source          1..6565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      679..1267
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1168..1187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1421..1438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  1570..1794
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1861..2394)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2583..2588,2589..2605,2606..2611))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2963..3054
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2979..2996
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3121..3138
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3160..3180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    5116..5250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5153..5172)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5207..5226
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5279..5444
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5628..6083
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5715..5734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5925..5946
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6110..6214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6399..6565,1..210)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(6433..6452)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
       61 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      121 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      181 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      241 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      301 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      361 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      421 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      481 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
      541 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      601 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      661 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      721 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      781 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
      841 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      901 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      961 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1021 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     1081 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1141 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1201 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1261 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1321 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1381 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1441 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     1501 cagaccagcc gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg gatgccctgc
     1561 gtaagcgggt gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga
     1621 caataaagtc ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg
     1681 aaaaagcata ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa
     1741 ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg taaagactat attcgcggcg
     1801 ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt gaacgaattg
     1861 ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt
     1921 tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc
     1981 accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct
     2041 ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa
     2101 cctgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag
     2161 cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg
     2221 ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga
     2281 tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa
     2341 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc
     2401 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag
     2461 gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac
     2521 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt
     2581 acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc cgtttccacg
     2641 gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg tcctggctgg
     2701 cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc ttgctgttct
     2761 tctacggcaa ggtgctgtgc acggatctgc cctggcttca ggagatcgga agacctcggc
     2821 cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc
     2881 tggaaggcga gcatcgtttg ttcgcccagg actctagcta tagttctagt ggttggctac
     2941 gtatactccg gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat
     3001 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg
     3061 gattattcat accgtcccac catcgggcgc ggatctcggt ccgaaaccat gtcgtactac
     3121 catcaccatc accatcacga ttacgatatc ccaacgaccg aaaacctgta ttttcagggc
     3181 gccatgggag atagcaaagt gaaagtggcc gttagagtta gaccgatgaa ccgtagagag
     3241 attgacctgc atacaaagtg tgtcgttgat gtggaggcta acaaggtcat cctgaaccct
     3301 gtgaacacca acctgagcaa gggagacgct cgcggtcagc ccaagatctt cgcctacgac
     3361 cactgcttct ggtccatgga cgagagcgtg cgtgaaaagt acgctggtca ggaagacgtc
     3421 ttcaagtgcc tgggcgagaa catcctgcag aacgccttcg acggatacaa cgcttgcatc
     3481 ttcgcctacg gtcagaccgg ttctggcaag tcatacacta tgatgggtac cgctgaccag
     3541 cctggcctga tcccaaggct gtgctccggt ctgttcgaga gaactcagaa ggaggaaaac
     3601 gaggaacagt ctttcaaggt cgaagtgtca tacatggaga tctacaacga aaaggtgcgc
     3661 gacctgctgg acccaaaggg ctccaggcag accctgaagg tcagagagca cagcgtgctg
     3721 ggtccatacg tcgacggcct gtccaagctg gctgtgacta gctacaagga catcgaatcc
     3781 ctgatgagcg agggtaacaa gtctcgcact gtcgctgcca ccaacatgaa cgaggaatcc
     3841 agccgttcac acgccgtgtt caagatcacc ctgactcaca ccctgtacga cgtcaagtct
     3901 ggcacctcag gagaaaaagt gggcaagctg tccctggtcg acctggctgg aagcgagaga
     3961 gctactaaga ccggtgctgc tggcgaccgt ctgaaggaag gatcaaacat caacaagtcc
     4021 ctgaccactc tgggtctggt catctccgct ctggccgacc agggagctgg caagaacaag
     4081 aacaagttcg tgccctaccg tgacagcgtc ctgacttggc tgctgaagga cagcctgggt
     4141 ggcaactcta agactgctat ggtggccacc gtctccccag ctgccgacaa ctacgacgag
     4201 actctgagca ccctgcgtta cgctgacagg gccaagcaca tcgtgaacca cgctgtggtc
     4261 aacgaagacc ctaacgccag gatcatccgc gacctgcgcg aggaagtcga gaagctgagg
     4321 gaacagctga ccaaggctga agccatgaag tctcccgagc tgaaggacag actggaggaa
     4381 tcagaaaagc tgatccagga gatgactgtg acctgggagg aaaagctgag gaagaccgag
     4441 gaaatcgctc aggaaagaca gaagcagctg gagtctctgg gaatctcact gcagacttcc
     4501 ggcatcaagg tcggagacga caagtgcttc ctggtcaacc tgaacgctga cccagccctg
     4561 aacgaactgc tggtgtacta cctgaaggag cacaccctga tcggttccgc taacagccag
     4621 gacatccagc tgtgcggcat gggaatcctg cctgaacacg gtatcatcga catcactcca
     4681 gagggacagg tggtcctgac cccacagaag aacactcgca ccttcgtgaa cggttcttca
     4741 gtctccagcc ccatccagct gcaccacgga gacaggatcc tgtggggtaa caaccacttc
     4801 ttcagactga acctgcctaa gaagaagaag aaggctgagc gcgaagacga ggaccgtgaa
     4861 gcctctctga agaacgactc ttcatccgag cagctggacg ctgacggtga cagctcttca
     4921 gaggtgtcca gcgaataagc ggccgctttc gaatctagag cctgcagtct cgaggcatgc
     4981 ggtaccaagc ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag
     5041 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     5101 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5161 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5221 ctcatcaatg tatcttatca tgtctggatc tgatcactga tatcgcctag gagatccgaa
     5281 ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta
     5341 cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact
     5401 ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt
     5461 cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc
     5521 tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt
     5581 aattgactga atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgtag
     5641 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5701 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5761 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5821 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5881 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5941 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6001 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6061 caaaatatta acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6181 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6241 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6301 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6361 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6421 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6481 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6541 gcatcttacg gatggcatga cagta
//
