LOCUS       sequence-31790-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector with TRE promoter driving both mouse G alpha 12 and G 
            alpha 13 miR30-based shRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_TmiR_G13-L_G12-E
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           80..201
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           229..243
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           271..397
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           456..577
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           605..619
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatc tagtcgacta
       61 gggataacag ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac
      121 atcttggaaa cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagaag
      181 gtatattgct gttgacagtg agcgatgggt gagtctgtaa agtatttagt gaagccacag
      241 atgtaaatac tttacagact cacccagtgc ctactgcctc ggaattcaag gggctacttt
      301 aggagcaatt atcttgttta ctaaaactga ataccttgct atctctttga tacattttta
      361 caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattcgcgg ccgcactcga
      421 gatatctagt cgactaggga taacagggta attgtttgaa tgaggcttca gtactttaca
      481 gaatcgttgc ctgcacatct tggaaacact tgctgggatt acttcttcag gttaacccaa
      541 cagaaggctc gagaaggtat attgctgttg acagtgagcg acgacaccat cttcgacaac
      601 attagtgaag ccacagatgt aatgttgtcg aagatggtgt cgctgcctac tgcctcgcaa
      661 ttcaaggg
//
