LOCUS       sequence-317906-        6100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses psychLight2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Dong C, Ly C, Dunlap LE, Vargas MV, Sun J, Hwang IW, Azinfar A, Oh 
            WC, Wetsel WC, Olson DE, Tian L
  TITLE     Psychedelic-inspired drug discovery using an engineered biosensor.
  JOURNAL   Cell. 2021 Apr 8. pii: S0092-8674(21)00374-3. doi: 
            10.1016/j.cell.2021.03.043.
  PUBMED    33915107
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Apr 8. pii: S0092-8674(21)00374-3. doi: 10.1016/j.cell.2021.03.043."
COMMENT     Sequence Label: pCMV_psychLight2
FEATURES             Location/Qualifiers
     source          1..6100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1362..1383
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1489..1510)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1750..1769)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2524..2645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2561..2580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2615..2634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2652..3107)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2789..2810)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3001..3020
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3134..3238
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3240..3597
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3240..3431
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3263..3283)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3448..3583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3632..4426
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3632..4426
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3686..3705)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4296..4315
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4614..4633)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4658..4705
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5034..5622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5523..5542
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5797..6100
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttaccggtg cgcgccatga agacgatcat cgccctgagc
      301 tacatcttct gcctggtgtt cgccgactac aaggacgatg atgacgccat ggacatactt
      361 tgtgaagaga atacttcact ctcttctact actaactctc ttatgcaact gaacgatgat
      421 acccgattgt actcaaacga cttcaattcc ggcgaagcga acaccagtga cgcattcaac
      481 tggactgtcg attctgaaaa cagaactaat ctgtcatgcg agggttgtct tagtccctct
      541 tgtctcagcc tgttgcacct ccaggaaaag aactggtcag cactgctcac tgcggtagtg
      601 ataatactca ctattgctgg caatattctc gtaattatgg cagtctcctt ggagaagaaa
      661 ctccaaaacg ccacaaatta ttttcttatg agccttgcca tcgcagatat gctcttggga
      721 tttttggtga tgcctgtgag tatgctcacg atactgtatg gatatcgctg gcctctgccg
      781 tctaaacttt gcgctgtgtg gatttacttg gatgtccttt tttcaactgc gagtattatg
      841 catctttgcg ccattagtct tgataggtat gtggctatcc aaaatcctaa gcaccattcc
      901 cgctttaata gtagaactaa ggcttttctg aaaataatag cagtgtggac catatctgtc
      961 ggcataagca tgcctatccc cgtatttgga cttcaagatg actcaaaggt attcaaagaa
     1021 gggtcatgtc tgctggccga tgacaatttc gtgcttattg gcagcttcgt cagtttcttc
     1081 attcctttga caatcatggt gattacctac tttcttacga ttaaatcttt gcaaaagcag
     1141 ctgagctcag gttataacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg
     1201 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag
     1261 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg
     1321 cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1381 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg
     1441 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1501 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
     1561 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1621 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     1681 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc
     1741 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1801 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1861 cacaagctgg agtacaactt taatatgcat gaccaactga atgagcaaaa ggcttgtaag
     1921 gtactcggca tagtcttctt tctgtttgtg gtgatgtggt gtcccttctt tataacgaat
     1981 atcatggcag tgatctgcaa ggaatcatgc aatgaggatg tgatcggggc acttctgaac
     2041 gttttcgtgt ggatagggta tctgtcaagt gctgtgaacc cactggtcta taccttgttt
     2101 aataagacat accgctcagc cttttcacgg tatattcaat gtcagtataa ggaaaacaag
     2161 aaacctctgc aacttattct tgtgaacact atccctgccc tggcttataa gtcatcacag
     2221 ttgcagatgg gccagaaaaa aaattccaag caggacgcga agacaacaga caacgattgt
     2281 agtatggttg ccctcggcaa gcagcacagt gaagaagcga gcaaagacaa tagtgatggc
     2341 gtaaacgaaa aagtcagttg tgtattctgc tacgagaacg aggtgtaaca acctgatgta
     2401 cagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     2461 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2521 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2581 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2641 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     2701 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2761 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2821 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2881 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2941 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3001 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3061 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     3121 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3181 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     3241 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3361 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3421 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3481 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3541 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     3601 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     3661 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     3721 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     3781 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     3841 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     3901 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     3961 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4021 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4081 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4141 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4201 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4261 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     4321 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     4381 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     4441 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     4501 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     4561 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     4621 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     4681 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     4741 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     4801 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     4861 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     4921 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4981 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5041 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5101 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5161 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5221 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5281 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5341 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5401 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5461 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5521 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5581 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5641 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5701 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
     5761 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     5821 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     5881 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     5941 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     6001 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     6061 ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
