LOCUS       sequence-317907-       21965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele 
            targeting vector, low copy number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21965)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Miscellaneous gene targeting vector plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA
FEATURES             Location/Qualifiers
     source          1..21965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        207..706
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(242..265)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     639..658
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             733..1305
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          1382..1447
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1521..1745
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1807..1827)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1807..1825)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1862..1881)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1906..1954
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(2258..2275)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2258..2274)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2267..2289)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2483..2502)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2602..2624
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2662..2680)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2748..2852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2853..2921,2922..3713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3071..3090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3878..4423
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     4324..4343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4540..4557
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4707..4726
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4707..4725
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4968..4992
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        10736..11115
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11117..11394
                     /label=chicken beta-actin promoter
     intron          11395..12411
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     12335..12357
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     12419..12438
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    12466..12497
                     /label=rox
                     /bound_moiety="Dre recombinase"
     protein_bind    12499..12532
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        12533..12580
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12599..13402
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12662..12681)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13272..13291
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    13415..13448
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    13475..13699
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    13884..14005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    14117..14238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    14350..14471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    14494..14525
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             join(14534..14536,14537..15247)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             14534..15247
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(15278..15280,15281..15994)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             15278..15991
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(16052..16072)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(16470..16481)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    16632..16856
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(21941..21965)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgattcgagg ctgctaacaa atcgagcagt gtggttttca agaggaagca aaaagcctct
       61 ccacccaggc ctggaatgtt tccacccaat gtcgagcagt gtggttttgc aagaggaagc
      121 aaaaagcctc tccacccagg cctggaatgt ttccacccaa tgtcgagcaa accccgccca
      181 gcgtcttgtc attggcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
      241 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
      301 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
      361 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
      421 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
      481 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
      541 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
      601 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
      661 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcaggtcctc
      721 gccatggatc ctgatgatgt tgttgattct tctaaatctt ttgtgatgga aaacttttct
      781 tcgtaccacg ggactaaacc tggttatgta gattccattc aaaaaggtat acaaaagcca
      841 aaatctggta cacaaggaaa ttatgacgat gattggaaag ggttttatag taccgacaat
      901 aaatacgacg ctgcgggata ctctgtagat aatgaaaacc cgctctctgg aaaagctgga
      961 ggcgtggtca aagtgacgta tccaggactg acgaaggttc tcgcactaaa agtggataat
     1021 gccgaaacta ttaagaaaga gttaggttta agtctcactg aaccgttgat ggagcaagtc
     1081 ggaacggaag agtttatcaa aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt
     1141 cccttcgctg aggggagttc tagcgttgaa tatattaata actgggaaca ggcgaaagcg
     1201 ttaagcgtag aacttgagat taattttgaa acccgtggaa aacgtggcca agatgcgatg
     1261 tatgagtata tggctcaagc ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga
     1321 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     1381 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     1441 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cagatcctag
     1501 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     1561 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     1621 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     1681 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     1741 tatggcttct gaggcggaaa gaaccagctg gggctcgagg gggggcccgg tacccagctt
     1801 ttgttccctt tagtgagggt taattgcgcg cacgtttgcc cgggagatgg gggaggctaa
     1861 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     1921 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     1981 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     2041 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     2101 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga
     2161 atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgacc
     2221 caagcttggc tgcaggtcga gctcgcgaaa gcttggcact ggccgtcgtt ttacaacgtc
     2281 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     2341 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     2401 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2461 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     2521 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2581 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2641 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2701 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2761 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2821 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2881 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2941 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3001 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3061 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     3121 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3181 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3241 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3301 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3361 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     3421 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3481 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3541 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3601 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3661 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3721 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3781 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3841 tccactgagc gtcagacccc ttaataagat gatcttcttg agatcgtttt ggtctgcgcg
     3901 taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct
     3961 gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg
     4021 tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta
     4081 ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag
     4141 ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg
     4201 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa
     4261 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc
     4321 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg
     4381 tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc
     4441 cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg
     4501 taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa
     4561 gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg
     4621 ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact
     4681 ccgctagcaa cctggcttat cgaaattaat acgactcact atagggagac cggcagatcc
     4741 ggcgcgcctg caggcgcgcc actagttaat tatttaaatc gatgcgacgc tagcggccgc
     4801 gtttgctttt gtttcgtgtt ggtcaccacg gccgagtttc ctcgggaccc cgggcagctg
     4861 ctttacatcc caaagaccta tctgctcggc cggcctcgga acgcgagcct gcccggaaga
     4921 tccccgggta ccgagctcga attcatcgtc accatcacct cgaatcaaca agtttgtaca
     4981 aaaaagcagg ctttaaagga accaattcag tcgagcaaat agaatttaag gccagcctgg
     5041 ggccttgtga gggcctactg tgaccctcca aaaaataaaa caagattagc tgttgtggga
     5101 aagtagaagg acaaatgtga ggcaggagat gagagaggaa tgactggtca catgatggga
     5161 gtccagtgaa catgctggca ggtctgtaaa ttgttctctt atgggtttat ttgtgatgat
     5221 acatctagct ttaccagtgg gttgggattt ttgtttttgt ttttaactga tacagactta
     5281 tccaatcaaa actgatagac aaggggacta gaatagttct gtgcttcagg actccatatg
     5341 aaaccctgca ctaaatatcc caaaggctct tgctttagag aaactccaga aggaattttg
     5401 ttcatgagtt aggccctgtc tcatccttta taaaattcat tataaaatta tataatttca
     5461 ttataaaata aacaagaaca aaggatcctt aatggtaagt tctagtaagg tgtacgataa
     5521 ttgagttttc atgtatgtaa ttttaaaaga aaaatacaag gaacaagtgg aatcataggt
     5581 tctgaggtct ctgaaacatc tcttccccat tactaaggta tatttccagc catgctcttc
     5641 cctcttctta gccctatttc agggttgaga aacatgagaa cacacacagg cccattttac
     5701 agggtggcaa agatggattg aagttcttgg gacaatgact acttgcccct ttgtgttctc
     5761 ttgtagtagc tataggtcag aacattagaa ttacctctgc ccaagtaaac aaaatgggaa
     5821 ctctttaccc tgttgaagat ggcttcacct ggtaaagcta ggcttaagag cgagtgctgc
     5881 atagcccagt ggtcaagtgc tccaaagcat atgtaagacc ctaggcttaa ttcccagtgt
     5941 cctgaaaaga taaagcactg gcttagtgtt aggcaaaggc acttgagtgt gctgatctct
     6001 gcctcttctc tgcagctttc tccttccttc acccctacct cagtgatccg gaagatgtat
     6061 gaaagcagag agaaaaccaa ggaggaaatg gcacctggaa tggtggttcc tggtgatggt
     6121 aaagaggata ctcagaagac cagcgaaggt actgctgctt acctgtgtca gaacataggc
     6181 atgagtgact gcagtgtcag ggagatgggt tagtgagctc aaacttgctc acttgacatt
     6241 gtggccagcc ggggtaacga tgtgctttag cttatgattc ttaccatctt tcaagttgaa
     6301 taacaaactc atttagcata ttgatatagc tcaattcttt ttttaacgta tttgtgtatt
     6361 tgcctgtggt atgtctgcat catgtgtatc cagtgcccaa ggaggccaga agagggcatc
     6421 agaacctcta gaattgtgag ccacatgtgg gtgctgggaa gaggtcctct tcaagagtat
     6481 ccaggtagat agggggatag atgcagggtt gttgttgttt ctgttacagg ttgtttgttt
     6541 tatttgcaac aggatcttac tgtgtagctg tggctaacct caaactcaca gagatctact
     6601 ggtctctgcc tcccaagtct taggattaaa ggtgcctcaa ctacatgcct ggcaacttgt
     6661 gctttttgtt ttgtaacact atgtcagagg tttttgagtt attcctttat gcaccgggaa
     6721 tgcagctcaa ttgtactaag tagtagaata cttgccagca tgtacaagac catgggttca
     6781 tgttcactcc ccaagaaagg aaatgacaac atcaaaaata attatttcag caaagaattt
     6841 gaagaccttg gcatctatgg actggagaga tagatcaggg cactgtttgc tcttccagag
     6901 gacctgggtt caattgccag cacccacatg atagctcacc ttttgtgact tcagccctag
     6961 gtgacctagc accctcccct ggcctctgtg agcactgaac tcgacaggca cacagacata
     7021 catacaggca aaacaccatc catatacatc ataatataag aaaataaggt tataaataaa
     7081 atgtgggcat ctattcctag ctcaaggcct aactactcta tgaccataac tcaactccgg
     7141 ggcttatgga gagtaccagc tgtgcatctg tctacattta aagcaaatag atgactttcc
     7201 atgaaagcat ctaacatggt taagaggaaa aaagagccgg catggtggca cacgccttta
     7261 atcccagcac ttgggaagca taggcaggtg gatttctgag ttcgaggcca gcctggtcta
     7321 caaagtgagt tccaggacag ccagggctat acagagaaac catgtcttga aaaaccaaaa
     7381 aaaaaaaaaa ggaaaaaaga attgtcagaa tttttaaaag taagatttgc tgggttgttg
     7441 ggaattgaaa taaattgata aaggatatca aaatgtcctg taaatgaatc cttacagaga
     7501 acctgctgtc atccaatccc atacccaata ctgatcaaga ctcttctacg acaaatccta
     7561 agctttcaac attacagcgg tcttcatgtt ccaccccact ctcccagacc agccgttata
     7621 ccaaagaaca agattatcga cctaaaacag caggaaggaa aacacctacc ttggcatccc
     7681 cagtcccagg aacacctttt ctccgcccta cccaccaagt tccccttgtc cctcatgttc
     7741 ctattgttcg acctgcacac cagcttcacc caggattagt ccaaaggttg atagcccaag
     7801 gggtacatcc acagcatctt ccaagtttgc tccaagctgg tgagtttctg actgggtgtg
     7861 ggcattggtt attggtcgta gtataaaaat tgctgccact aattaaatgt tacattatac
     7921 taagtattcc aaaaagatct tactagattt aaattcttct acctaagtag tgagtccaat
     7981 ccagtctcac ttccttagct cacaaaaaga ttaggaaacc tgccagacag tggtaataca
     8041 cgcctttaat ccctgcactc aggaagcaga ggcaggtgga tctctttgag ttcaaggata
     8101 gccagggtac actaaggaac cctggctgtg gcgttcattt acaattgtgt ctgtgtatgc
     8161 atattcatgt accctggtac atgtatggtg gcaagtgttc accacatcca cctccttgta
     8221 agtatccaaa tcaacaaaag tagtaaactt tgacattttt aggaacatct acaactcatg
     8281 agtttcttct ttctccccat caggtgtgct tcctcctggg atagacatgg ctcctttaca
     8341 ggggctatct ggcccactcc tgggtcaacc cttgtaccct ttggtctctg ctgctagtca
     8401 ccctctccta aaccctcgtc ctgggacccc tctgcatttg gcggtgatgc agcagcagct
     8461 acagcgctca ggtaaggttt agaaataggc caggttagct gatcacactg gaaagttctg
     8521 agatttccag gagcaatagt tatcactgcc cctttgtttc ttaggtgtca ctagaaaaac
     8581 aggtgaagtg aactgacctg tgcagtctca gggattatta ctactgcaag gacagtcgga
     8641 gccagccatg ctctagctct gtttttcact tcttataagc ctgagaattt ttgctgagat
     8701 gtgaacatgt cagccttggt gggctttctt acgttgcatt ttcttacgtt gcattttgca
     8761 ctttaggaag cctttggtta aatcctctgg tagacttccc atcccccaaa gtaacaaacc
     8821 attcaagcaa agtaggtcag aaaatattgt caatactgag aacaggactt gggagtgagt
     8881 atccaagggt ggtcttagta gacttgtggg gttctgtggg tgaggtgtag agtagagcta
     8941 ctttgctgca ctgacactct tctctccata gttctgcatc ctccaggctc tagttcccag
     9001 gcagcagcta tcagcgttca gactcctcag aatgtaccca gccggtcggg catgccccac
     9061 atgcactccc agctggagca tcgtaccagc caaaggagca gctcccctgt gggccttgcc
     9121 aaatggtttg gctcagatgt gctacagcag cctctgccct ccatgcccac caaagtcatc
     9181 agtgtagatg aactggaata tagacagtga agagggcagg ctggctcacc catacctgga
     9241 cctgtggtga caccctggtc atgactctca ttccctcttt gtaatgggct tttacattgg
     9301 agcacactat gtgaagatgt ttaggggatc cacataccta ccatgatcta cattatgaca
     9361 gaaggctgtt aaatcgaatg aacctacatg gttcaaatac aagggataca agattgtcag
     9421 tcctggaagt ctttctttta taaaatatgt gaatgaagtg ttggtgtctt ctagaggtga
     9481 cacctaaggg ttctgaaaaa ataaaatgta tagaccctta tgtacagacc tgtgtataaa
     9541 cttttgtaca tacaaatagg gtagcttttt ttgaacttat acatacagct gtacataaag
     9601 taactatcag ttaggcttgt gtcaactgtt tggatttttt tcacttgaat atttgggact
     9661 ttttcttttg gtttattaaa agttacatat gccacgtgtg tgaacgatat ggctggtact
     9721 gtgtttattt cttccatgaa ctaagacagt ctaaatgagt tcctttcacg ttttaatttt
     9781 accttaggac ttctggaatt tcttctgcac ataaagttct gatagcatta gtttaagctg
     9841 gactaaccct gaaagtagct tgtggcaagt atcaaggaat caatattata ctctacaaaa
     9901 tcaaagttta cagagaagtc atatagtaat ttttctgaaa tttactggca caatgttaat
     9961 ccagcctgac tccaactaat taatggtcac attaatttaa gtctttccct tgcctctgct
    10021 gcattagttt ctctcaaaat tgttaactta caacttgaag tctggtatta taaattgaat
    10081 gtaaagcatt ctgaaagata ctatactgat tgcaggtttt tcagtcaggt tcaagctaat
    10141 ttgaccagtc attggattaa ttatggatct ggggccataa atgctatttt aattccacta
    10201 tagagattaa aataagccat tctccatttc ataatattct attggacttt gactgcaggg
    10261 gcctccaagt cttgacagta gattataatc cttcagctgc ccactctact ggaggaggac
    10321 aaactggtca cttttcagca aaacctggct gtggatcagg gcagtctggt acttccaagc
    10381 tcattagatg ccatcatgct ctcactgcct cctcagcttc aagaggaatc tggaaaaagc
    10441 agtcccactg gtcaggaaag gaacactagt gcacttatcc tgggtgtctg ctgagccaac
    10501 agtggtagta aggtaagggc aggatgtgtc aaactgccaa tagagaacta cttactcttc
    10561 aggctgaagc tgatggaaca ggtaacaaag gcaaacacta atcatgatca gcaagatgaa
    10621 gcagaaaggg aacaagggga tattaaatgt gtatagacac gctagagaga tggctcagca
    10681 gttaagagaa ctagctggtc tttcagaggt cctgagatca ataccggtca tggtcgacat
    10741 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
    10801 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
    10861 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
    10921 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
    10981 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    11041 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
    11101 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
    11161 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
    11221 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
    11281 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
    11341 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
    11401 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
    11461 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
    11521 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
    11581 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
    11641 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
    11701 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
    11761 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
    11821 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
    11881 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
    11941 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
    12001 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
    12061 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
    12121 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
    12181 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
    12241 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
    12301 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
    12361 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
    12421 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattctaact ttaaataatt
    12481 ggcattattt aaagttaaga agttcctatt ctctagaaag tataggaact tcgttgacaa
    12541 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
    12601 gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
    12661 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    12721 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    12781 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
    12841 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    12901 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    12961 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    13021 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    13081 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
    13141 gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
    13201 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    13261 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    13321 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    13381 ccttcttgac gagttcttct gaggggatca attcgaagtt cctattctct agaaagtata
    13441 ggaacttcgc tagcagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
    13501 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
    13561 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
    13621 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
    13681 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gactagagct
    13741 tgcggaaccc ttaccaccgc ggtggcggcc gctctagaat cataatcagc cataccacat
    13801 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    13861 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    13921 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    13981 tgtccaaact catcaatgta tcttaactag aatcataatc agccatacca catttgtaga
    14041 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    14101 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    14161 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    14221 actcatcaat gtatcttaac tagaatcata atcagccata ccacatttgt agaggtttta
    14281 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    14341 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    14401 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    14461 aatgtatctt aactagtgga tctcatgcct aggtaacttt aaataattgg cattatttaa
    14521 agttaggcgc gccatgagca aaggagaaga acttttcact ggagttgtcc caattcttgt
    14581 tgaattagat ggtgatgtta atgggcacaa attttctgtc cgtggagagg gtgaaggtga
    14641 tgctacaaac ggaaaactca cccttaaatt tatttgcact actggaaaac tacctgttcc
    14701 gtggccaaca cttgtcacta ctctgaccta tggtgttcaa tgcttttccc gttatccgga
    14761 tcacatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg
    14821 cactatatct ttcaaagatg acgggaccta caagacgcgt gctgaagtca agtttgaagg
    14881 tgataccctt gttaatcgta tcgagttaaa gggtattgat tttaaagaag atggaaacat
    14941 tcttggacac aaactcgagt acaactttaa ctcacacaat gtatacatca cggcagacaa
    15001 acaaaagaat ggaatcaaag ctaacttcaa aattcgccac aacgttgaag atggttccgt
    15061 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
    15121 agacaaccat tacctgtcga cacaatctgt cctttcgaaa gatcccaacg aaaagcgtga
    15181 ccacatggtc cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct
    15241 ctacaaaggt tctgctggat ctgcagcagg atctggtatg agcaaaggag aagaactttt
    15301 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
    15361 tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg
    15421 cactactgga aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt
    15481 tcaatgcttt tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat
    15541 gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac
    15601 gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat
    15661 tgattttaaa gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca
    15721 caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg
    15781 ccacaacgtt gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat
    15841 tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc
    15901 gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg
    15961 gattacacat ggcatggatg agctctacaa atgattaatt aaaacctctg gattacaaaa
    16021 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
    16081 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
    16141 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
    16201 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
    16261 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
    16321 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
    16381 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
    16441 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
    16501 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
    16561 ggatctccct ttgggccgcc tccccgcagg atccactaga gcggccgcct agagctcgct
    16621 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
    16681 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
    16741 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
    16801 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
    16861 tgaggcggaa agaaccagct ggggctcgac tagagcttgc ggaaccctta agcttgtcat
    16921 tcgaatttag acacccacat ggtggctcat gaccatctat ctataaatgg atctgatttt
    16981 catgtctggc agtgtacaga agctaactga agaaaggtgg aagacccaca agagttcaag
    17041 ataagcccta tatagtgaag ttcaaggcaa gctttttcta cctgaaactt agtctcaaaa
    17101 aaaaatgaat acgtaaacag tcttccaggg gataagaacc ttacagaaaa agcagaaatg
    17161 cctggggcac tggattaccg atgtaatcaa attcagtcct tgaattgaac acaggattgc
    17221 ctagagcaag gccagccaga gattcatctc agagggagaa aggtgtcttt ggagcaattt
    17281 tgtggtaatc tagtatgtat cacataagtt tagacgcatt tgggactgga aagatgtgaa
    17341 caaagcaccc tatggctcac atctgtcatt aactctagtt ccagtgcacc tgacaccgtc
    17401 ttctggcctc tgcagtgacc aagcacatgg gtagtatgta gacatataca taagcaaaac
    17461 acacatcatt aaaaagtgac atttcccaaa ggaagctgaa gaaccagttc ttgagaagat
    17521 agtagaaatc agaaggggaa atagtagaca tacagaggga ctgaccaggt tgtgtcacct
    17581 ttataggcta ggctaatgga tgatcgacac tagcgctctt tgtgaaggac acacaaatga
    17641 gacatagttt ataggactaa acacacttct aagcaattta atgagactta agaccctgtc
    17701 tctagcaaat actctggatg atattcagct caaggctctt gtcagacatg tttccatttt
    17761 caaggtgagc taactggcca aaactgccaa caacctgtag tgaatagaga agatgagaaa
    17821 atctggattc tcaaatgacc taatgaaagc cactggagcg ccatatggtt tctgtgaaaa
    17881 tgccttttca atcattaacc tcttaaatga gtgttagcat cctaactaat gagtggtgca
    17941 gaatagtggg tctgcttagc ttaactaagg ccaagaaaac aaaacaggaa attcattcca
    18001 tgtcatgaga ctcatactac gaggttccct tagacctcag gagaaaaagt ctttggctgt
    18061 aagaacacac ctcagtggat gtggtagact atgcctttac tctttttttt tttttttttt
    18121 ttttttgggt tttttcgaga cagggtttct ctgtgtagcc ttggctgtcc tggaactcac
    18181 tttgttgacc aggctggcct cgaactcaga gatccgcctg cctctgcctc tcaagtgcta
    18241 gaattaaaag tgtgcgccac cactcccagc tatatagact atgcctttaa tcaggacact
    18301 tgggaggcag ggggatctca aattcaattc cagacagatg actgacaaac acacacatac
    18361 accattcgtc ttttcttttt ttcttttttc ttttgaaaac aggtttctct atgcagccct
    18421 ggctttccta gaattcaatc tatagaccca gctggcctca aacacagaac ctcttgcctc
    18481 tgcctcctca acactatgac taaaggtgtg aaccaccacc accaccaccc acctggccaa
    18541 agaatgaatt gagtgaataa gtacgagtgc aactctccag cagcctgagg caagaaagct
    18601 taacagttga acctgagacc ctacttcgtt tgtgttgtac atatcttctc ataatgaact
    18661 aggcagccta gtctctctac caaatacacc atgcacttac acctcctcaa accaactgca
    18721 acagctacac acatttccat ggcttgcctg taataataaa ttcacaaact tcctctaagt
    18781 aacttttagt ggaaatctac caggttatgc ctatttgtga ttgggtgcac agatgactag
    18841 atgagatgta atttgggaag tgacatgcaa taggaaaaat gaatatataa cctataaata
    18901 gaaaacacca aggcaagcgt ggtgactcac acctttaatt agcacttggg aggcagaagt
    18961 cgcatcatct cttaggtcaa ggccaatcta gttccagacc agcaaggaca acagaaacct
    19021 tgccttgata taaacatttt aaaactttaa aaatagagaa gcccaaactc tgctcattgg
    19081 taatctaccc ttcctttctt tagacacctt gagaagctag gctacatagc tttgggtcct
    19141 tacgtacccc cactcactca gcctgcagtc aatctctgat agtgtttcct gccataagct
    19201 acactactgt tttgctccaa accttacttc tagtacaaat ccatgtgagg tcttcgtttc
    19261 aatttggggg ggaagaggcc agaacacttg ccgcagatcc caacaacggg cagcacattc
    19321 ccagctagtg tatctgatac aaactctatc ttcattcaga accatcaagt ctaagcccca
    19381 agagcaaagg taaattcatg tatgtttggc tggagaaagc atttcaagtc aaggtgagcc
    19441 ttgaactcct aacactcttg cctctacctt ccaaatgcca atataggtat ttgtcacacc
    19501 agcttctcaa atcctgttgg gctagcaact gggtgggagg agtgtagagg ggtactaaaa
    19561 aatggactca aggcctcttc aaaaccactg aggccttgta caggatttga acttccttct
    19621 cttttcagca tcctgaagta caaagctaaa gcaaagtaaa tttttgcttg gcctgaggac
    19681 ttccaaaaat ctaggctctc actggagaag aaacctagct ggaaattcca cagttctgat
    19741 aagccaattc tttttttttt taaccattta tttatttata agtaagtaca ctgtagctgt
    19801 cttcagacat cccagatctc atgacggatg gctgtgagct accttgtggt tgctgggatt
    19861 ttaactcagg accttcagaa gagcagtcag tgctcttaac cagtgctctc cggcccagtg
    19921 gaaaccaatt ctttagtgtg gggacaggct ttcagtcata gcctatcagt caatgtccta
    19981 gtgagaaacc agtctatatt ttgaggcttg agaggcctga ttccaaggac agttttaaac
    20041 ccggagaaag gaagagagct agctctaggg cagcagacgg agccctgagg gaagggtagt
    20101 gtgtggtaag gcccacagct ttcctcagac ctctgaaggc tctagctcat ctcctttgaa
    20161 gacagaagaa gcaaagtctg ttctcctaca aagacgttcc tgtctttctc aaggcctcct
    20221 ctatcatcac tgcagaccac attaaaataa tcttccagcc aggcggtggt ggcacacacc
    20281 tttaatccca gcacttggga ggcagaggca ggccggatct ctgagtttga ggccagcctg
    20341 gtctacaaag tgagttccag gacagccaga gctatacaga gaaaccctgt ctcgaaaaac
    20401 caaaaaaaaa aaaaaaaaaa aaaaaaaaag attactgtat ttgtgagcct caatctcatc
    20461 gtgtcgtaca tggaacagtg gcattaactt ttaaggttgg gattgctggt attaagggga
    20521 gggttttgga agctgcttag cacagtgtca gtcgtaggaa atattcactg tatggtggtt
    20581 cttgtcactc agcctgtttt tgtttttgtt ttgttttttg tttttttcct gagacagggt
    20641 ttctctgtag tgttnntata tatatatata tatataactt cagacagcaa aagagggtat
    20701 cagaaagggc taatagacaa cttgcacctc accctatagg tggaacaaca gtatgaacta
    20761 accagtaccc cccggagctc gtgtctctag ctggatatat agcagaagat ggcctagatg
    20821 gccatcagtg gaaagagagg cccattggtc ttacaaactt tatgtgcctc agtacaaggg
    20881 aacaccaggg ccaagaaagc gaggggaggg gggttgggag actttcagag tagcattgga
    20941 aatataaatg aagaaaatac ctcctcatca cagcagtccg ctcagctcag aatccagaga
    21001 ctattgcgct ggagaaaaat ctgcagttaa ctttacctat tataccttcc tctctccaat
    21061 gtcctgagaa ggaccacttg aaaggacaga aagccaagcc caggtgaaag cctatcatta
    21121 cagcacacta ccaatggaca aacggacaca ggtcccattc tctgtcccat ttcctatgac
    21181 caagcaggcg ttgcctcatt cacctcatca cacagcaaac ctcacatgaa taacgacaca
    21241 tggccagcgg gcccaccagc ttctttatac agttcaatag aagtgacagc aaagtaggga
    21301 aagtggtgtt gctgaggagc taaaggtgca cagtggtaag cccagcactg aggagatgga
    21361 ggctgatgaa agggattcta ggccagcctc agctgcaaga gttcaaagcc agcctgaaat
    21421 acataacacc ctatctccgg aaagaaaaaa attgtggtgt ttcagtttga taagtctcca
    21481 tatcaatccc tgtatcccaa tccagaggct gccaaagaca gaggggctga ggaaggtact
    21541 tagtcataag gaacactgct gtggccagcc agcagatggc aagaggactt cacttcttca
    21601 caatctgaat gacgtcttca tcttccaatg tgtggtcttt acccactttc tgaggattat
    21661 gtttcacaga cagaccccag accaaagcac tgtatggaga ggaataaaga gtcagtggct
    21721 gtagcaagca gctgcttccc taactgccca ggtgccagct tatcaaattt agcatcctgg
    21781 atcttaaaag gcacagaact acaacagata aaagccctct tctaaataca tcagaataaa
    21841 caaacagtca aagccacttg ctaaagcagt gttgcacact gagtcatggg atcgctgtca
    21901 ttcaacagcc aattagttta aaccaactgg agatatctag acccagcttt cttgtacaaa
    21961 gtggt
//
