LOCUS       sequence-317909-       19494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B6J Csrp2-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy 
            number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19494)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Miscellaneous gene targeting vector plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pB6-Csrp2-T2A-sfGFP-iCre-ERT2-FNF-DTA
FEATURES             Location/Qualifiers
     source          1..19494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4222..4275
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4291..4293,4294..5004)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4291..5004
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4321..4349)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4944..4964
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             6133..7068
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6181..7074
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     protein_bind    complement(7107..7140)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    7195..7419
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(7457..8260)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7568..7587)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8178..8197
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8279..8326)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(8338..8837)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    complement(8901..8934)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     gap             9422..9432
                     /estimated_length=11
     protein_bind    complement(14478..14502)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        14709..15208
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             15235..15807
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          15884..15949
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    16023..16247
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(16309..16329)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16309..16327)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(16364..16383)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    16408..16456
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(16760..16777)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16760..16776)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16769..16791)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(16985..17004)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17104..17126
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(17164..17182)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        17250..17354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(17355..17423,17424..18215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(17573..17592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      18380..18925
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     18826..18845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     19042..19059
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     19209..19228
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19209..19227
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    19470..19494
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttaaaggaac caattcagtc gacatatttt aatgatgttt aaaatctatc taccctgggg
       61 cttagtggtt tgcaggaaaa atttagacag cacaacagtg gcgattcatg atgaagagat
      121 ctactgcaaa tcctgctacg gaaagaagta tggaccaaaa ggctatggtt atggccaggg
      181 cgctggcacg ctcaacatgg accgcggtga gagactgggc atcaagccag agaggtgaga
      241 gaatgttact ctcttaaaag cgggtagaac agctcctgtc gctcaggcac caggagcctg
      301 catatttagt ttaaactaag caagcaaaat aaaatgtgac ctctactaaa tactcatatg
      361 attactacga cgttctgtaa cgtcataata ttgacagttt tgtatctaaa aatcttagta
      421 atgaatgcag ggacttctag ccctggttat atagcatttt aactgatatc aggaaaacat
      481 aaatctcaag gaacgtgact tacattaata tcccatagca ctctggagat caaatatctt
      541 gaaatgagtg tctgaattct gagatgcttc tcatatgcaa ttaagctgtc cacggaaagt
      601 ccttagtcac tctttcctca ggaaattacg atccttcaac ttagaaatta aaaccatttc
      661 ctgcttctga tgatttgagg gacaaatgcg aagccagtct tgagttctga taaccaaagg
      721 tgtgtcactc tgataaccag agtgtcctcc gttcaggtca cctaatgctg cgttcatttc
      781 catgctcagg aaggtcactt ggaacgtcct ctatttcctt cctgctttcg tccctggttt
      841 tgaaacaggc tctccctgta tggtccagaa acagccaacc tacccctggt ggacactgtt
      901 gaaatgtact gtacttctta tattctcttt taagtgtatc cctgattcca aagggattcc
      961 aaatgtttca caatatacat tacctctaaa tcttcccatc aatgaaaact aaattcacaa
     1021 gacccccaaa ggctgtgttt gtagccagaa ctgggaaatc acggatgctc tttctgccct
     1081 gtccccacct ttcccagcaa taagcaagct ctgtgtgcac gccctatgtg cagtggtaac
     1141 cctgtctgtc ccttccagcc gggccctggt ctggtcttcc tctgcgatca ggtctaagga
     1201 attcctcctc ccagaggtct tctttaggac tcaaaaccat ggcctgcctt ttaacacaca
     1261 gattaaagag ctcacaaggg gctcctcgag aggtagcact gaggcgagta atttatccca
     1321 tcagcaagat atttaacatc tgggccccca ttttaaaacc tataacatga agtatcataa
     1381 cctgtgaagt tgttaaaaga ccagaggggt tgcgatctct gtaaagttct taatgacaca
     1441 ctgggaattt tcttcttggg actctaatct tctaagtgtt agatgtcagg tttgtaagtt
     1501 ttcaaagatc ataagtttgg gttggaaatg atttgaacat acaccagtgt tcgaacaact
     1561 gaagaaacac gctgtaaatt catgctttga atcagccaca cacacacaca cacacacaca
     1621 cacacacaca cacacacacg actccaagac agcaaactag ccagcagttc atggagaaaa
     1681 ctcccgtgtg actcaaacac agagtgaaga gaggtgaagg gacagcgaag tcttgaaaaa
     1741 tgatttaacg aaaaacaaaa tcagcatttc tatattaaga ttttgacttt acttatatga
     1801 ctattttaac aggtattata tagtgtggag ttgtcacgca gcgacctgtt gacttcaggt
     1861 tctgcaatgc cctcagccaa gagggtgttt tgcatattct agtgtgactg cttatgaatg
     1921 agggaaatca atcctgttaa ctatgattca ccctcgtaac ccgtcgtaaa cagtgataga
     1981 aataccatcc tgccacggga cactgtcagg caggacagca gagtaacagg taaacagtga
     2041 tagaaatacc atcctgccac gggacactgt caggcaggac agcagagtaa caggcaaaca
     2101 gtgatagaaa taccatcctg ccatgggaca ctgtcaggca gagtaacagg cacatttcca
     2161 aaagtatttc ccataaagga aacagttgag tcacacttta cctgctaaag atgcagtcag
     2221 caaataatct ggaaaacacg ccttccttcc ctagttgtga acgacttaga gtcagccgat
     2281 gtgtttgcgg gtagttttta atgccagaat tgcttacatt tgactttgca gtctctggtg
     2341 atacacaaag ctatgatcga gttatattgc tatgtctatg ggggagggat aactgcatag
     2401 ggaagctagg gacataatac agtctgttca cactcagagg gaaagatact tcagtttcag
     2461 gtttggcttg cctttttcta gtcctgatga atccagacac aggtaaagtt agaccatcca
     2521 tgagcacttg gtgtgtttat ttttggcaac ttctgacact gcccctgggc agcagtagag
     2581 ctccgaagct cctgttagct caggaggaac atttggagaa acactgcctc atttttttct
     2641 ccgttcctcc agtgctcaac ctcacaggcc tacgacaaat ccaaacactt ctaaatttgc
     2701 ccagaaatat ggaggagctg agaagtgttc caggtgtggg gattccgtgt atgctgcgga
     2761 gaagatcatt ggagctggga aggtagggcg ctgtctctaa gtggtaactg cagcacacac
     2821 tcacacacac acagggtgct gtctgtctct aactggtaac tgtaataaac acacacatac
     2881 atacacaagc atacatagac acacacacac acacacacac atacatacac aagcatacat
     2941 agacacacac acacttacac acacatacat acacaagcat acatagacac acacacacat
     3001 acatacacaa gcatacatag acacacacac acacacactt ccatttccca cttacgcagt
     3061 gtgttctggt ttaagattaa gcatttccag cagaatttcc attggaatca ttgatttctg
     3121 ggagtgaaag ccatgcttaa ctgtcatgtt taaaggaacg tgttttccct gacaatgccg
     3181 aactgcgaac aatctgctgt ggacaggttt tgcaggataa tcagggctaa gacaccagat
     3241 gatggctaga tccgtggact tggaaaaatt accacttatt tccttcgagc tgctcaattt
     3301 tgagcgagga aggttggcaa ctttgaagga gcagactggg tctgtggcaa ggccagagcc
     3361 tgctcccagg caaacagccc tttactggtg gctaggaagg atatggacag caaagagggc
     3421 cagctttcta gctgagccaa accgtagcct gaggaggctg cttgtgcgct ggttttccca
     3481 gccacttgct gcatctagat cataccaaag gaaacaagcc tctcaatgtc ctaactcagc
     3541 tgtctcttcc agccctggca caaaaactgt ttccggtgtg ccaagtgtgg gaagagtctg
     3601 gagtctacaa ctctgactga gaaagaaggc gaaatctact gtaaaggtaa aaactcggtt
     3661 ctgctgtctg ttagtgtcac cagaaaggga gacatcgtgc actgttacct tttgaaaatg
     3721 agaccgacat cttaggacag tgattacatt cttccattcc tactgtgtgt gttaagtccc
     3781 acacggctgg ggatctggcc gaatggtaaa acgcttgcct atgtagcaca ttcacaaggc
     3841 tgagcgccac gctcagcacg gcctccccaa cctctgactt cctgctttaa gccaagcata
     3901 tgactacgtg agggtgacac acagaaggca gctggatttc acgcctgcag ctcatcacaa
     3961 tcctaacttg gatgccgtgg gaattcctgg actcgcttca aacaaggatg ctcatagcag
     4021 agcccatttt atatcttaaa ctgacctctg gcagagcctc cagttggctt ttaaattcat
     4081 ggccatttgt tagtgacctc tgattaactc tccctttcct ttgtagggtg ctacgcaaag
     4141 aactttgggc ccaagggatt tggctatggt caaggggcag gggcccttgt tcatgctcag
     4201 ggaggatctg gcggaagatc tgaaggccgc ggcagcctgc tgacatgcgg cgacgtggaa
     4261 gagaaccctg gccctgctcc tggcagcaca atgagcaaag gagaagaact tttcactgga
     4321 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtccgt
     4381 ggagagggtg aaggtgatgc tacaaacgga aaactcaccc ttaaatttat ttgcactact
     4441 ggaaaactac ctgttccgtg gccaacactt gtcactactc tgacctatgg tgttcaatgc
     4501 ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc catgcccgaa
     4561 ggttatgtac aggaacgcac tatatctttc aaagatgacg ggacctacaa gacgcgtgct
     4621 gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg tattgatttt
     4681 aaagaagatg gaaacattct tggacacaaa ctcgagtaca actttaactc acacaatgta
     4741 tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat tcgccacaac
     4801 gttgaagatg gttccgttca actagcagac cattatcaac aaaatactcc aattggcgat
     4861 ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgtcct ttcgaaagat
     4921 cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca
     4981 catggcatgg atgagctcta caaaggagga tctggcggcc gctctgccac aaacttcagc
     5041 ctgctgaagc aggccggcga cgccgctgaa aatcctggcc ctgctcctgg aagcaccaac
     5101 ctgctgactg tgcaccaaaa cctgcctgcc ctccctgtgg atgccacctc tgatgaagtc
     5161 aggaagaacc tgatggacat gttcagggac aggcaggcct tctctgaaca cacctggaag
     5221 atgctcctgt ctgtgtgcag atcctgggct gcctggtgca agctgaacaa caggaaatgg
     5281 ttccctgctg aacctgagga tgtgagggac tacctcctgt acctgcaagc cagaggcctg
     5341 gctgtgaaga ccatccaaca gcacctgggc cagctcaaca tgctgcacag gagatctggc
     5401 ctgcctcgcc cttctgactc caatgctgtg tccctggtga tgaggagaat cagaaaggag
     5461 aatgtggatg ctggggagag agccaagcag gccctggcct ttgaacgcac tgactttgac
     5521 caagtcagat ccctgatgga gaactctgac agatgccagg acatcaggaa cctggccttc
     5581 ctgggcattg cctacaacac cctgctgcgc attgccgaaa ttgccagaat cagagtgaag
     5641 gacatctccc gcaccgatgg tgggagaatg ctgatccaca ttggcaggac caagaccctg
     5701 gtgtccacag ctggtgtgga gaaggccctg tccctggggg ttaccaagct ggtggagaga
     5761 tggatctctg tgtctggtgt ggctgatgac cccaacaact acctgttctg ccgggtcaga
     5821 aagaatggtg tggctgcccc ttctgccacc tcccaactgt ccacccgggc cctggaaggg
     5881 atctttgagg ccacccaccg cctgatctat ggtgccaagg atgactctgg gcagagatac
     5941 ctggcctggt ctggccactc tgccagagtg ggtgctgcca gggacatggc cagggctggt
     6001 gtgtccatcc ctgaaatcat gcaggctggt ggctggacca atgtgaacat tgtgatgaac
     6061 tacatcagaa acctggactc tgagactggg gccatggtga ggctgctaga ggatggggac
     6121 ctcgagccat ctgctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc
     6181 aaacgctcta agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc
     6241 ttgttggatg ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt
     6301 gaagcttcga tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc
     6361 aactgggcga agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt
     6421 ctagaatgtg cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac
     6481 ccagtgaagc tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta
     6541 gagggcatgg tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg
     6601 aatctgcagg gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg
     6661 tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc
     6721 ctggacaaga tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag
     6781 cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt
     6841 aacaaaggca tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac
     6901 ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca
     6961 tccgtggagg agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc
     7021 ttgcaaaagt attacatcac gggggaggca gagggtttcc ctgccacagc ttgaggcgcg
     7081 ccggatccat tatgtacctg actgatgaag ttcctatact ttctagagaa taggaacttc
     7141 gaagggttcc gcaagctcta gtcgagcccc agctggttct ttccgcctca gaagccatag
     7201 agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct
     7261 gccccacccc accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct
     7321 cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg
     7381 aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgagctc
     7441 tagagaattg atcccctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     7501 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     7561 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     7621 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     7681 catcgccatg ggtcacgacg agatcatcgc cgtcgggcat gcgcgccttg agcctggcga
     7741 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     7801 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     7861 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     7921 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     7981 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     8041 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     8101 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     8161 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     8221 aacctgcgtg caatccatct tgttcaatgg ccgatcccat ggtttagttc ctcaccttgt
     8281 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacaggc tgcaggtcga
     8341 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
     8401 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
     8461 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
     8521 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
     8581 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
     8641 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
     8701 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
     8761 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
     8821 aagcgcctcc cctacccggt agaatttcga cgacctgcag ccaagctagc ttggctggac
     8881 gtaaactcct cttcagacct gaagttccta tactttctag agaataggaa cttcggaatt
     8941 cgatatcaag cttatcgata ccttaattaa tggtgtgaac ccagtaagca cgacagagaa
     9001 tctccattac caaactgcag atggcgttta tggcgctcac tactgtgaaa cagccagcac
     9061 ttggcactgg gcatcaccga gctgcctgtg ggggctggac cgacagcgct gcactctccc
     9121 gcccactcac tagcgtctaa gagcattctt ttacatttga aataaaattt tggcttgatt
     9181 tgggtaccac ctcttaatta acctttcaga ggagctgttg tgatttttag atgatgagaa
     9241 gttatctggt tccttcctcc agtgaaaacc agtctcctga ttaaaaaaaa aaaaaagacg
     9301 ttcttaaaaa gacaatcaat tcctttatgc agtaggctaa catttgcact ctgagagctg
     9361 aaaacgacat ttttactttt gagattttca ttcatatata tatatatata tacatatata
     9421 tnnnnnnnnn nnatatatat atatatatat atataacaaa acactctttg ttgaaggtgc
     9481 gctcactggc agttttccag gcttccacac gcacacacaa atgcacagat ctctttagct
     9541 gtcaagatgc ttcgactgac tcatgattgc caagctcctt ggagccagca gttagcactc
     9601 agcatctctt tcccttctgc cccatttgag cctgctggtg gtgactcttt gtcaggacag
     9661 ctccctagag ggtagatgga aatgatctac attagtaaca cctgggtatg agactctggc
     9721 tggagaggtg gcttggtgac ttttgggtcc ccagtgccct gctgagctcc ggttcagaga
     9781 ccctgtctcc aaagtaaggt gatccagtga aacacctgat gtcaacctct gctactcacg
     9841 tgggggtagc aaggtcccca ctccccagag gcaggaccct aaataaagca ctgctggagt
     9901 cttggctgct gcacttcaca cagaccgtta ttactaaact gagcaacgtt ctagccctgc
     9961 ctaacaaatt ttaaaatcaa aaccaaacag ataaactttt tccaactaac ctaactgaat
    10021 tatggaacta agttcgaaat gtttacaaaa gtatctagca tgaataaaaa acaaaaatta
    10081 ctaggtctga aaagaagggg gaaactcatc aactgtacat ttctataatg agggtgggaa
    10141 ctatcaaaag ccactagaac tggccaagat gtaggggggg aaacaaggga tggaggttat
    10201 gtaaacactt accctgcagg gaaaaactca ggaaatgcta ggaatcgttc aaaaaagaaa
    10261 gagcattcag tcaacagtgg aagtcctaga aaccttgcta ccatgtgagg agccagaact
    10321 gcctgagagt tcttgggagg ggttgggggg ggggggcaga aaaatatcag aagaatgaca
    10381 gacatttcca aatttggtaa acttgtaaat ctatagatcc aagcattttg atactataaa
    10441 cgctaatcac taagctctaa aatagtgcct tctcttaaaa cttcatcaag gcaggagtct
    10501 gggtgcatat ttctcatcac tgctacctgg gaagcagggg ccggtgaatc catggccagc
    10561 cctcaacaga gcagctctgc atctccagca gatggggaca aagcgcacat ccaggtcaga
    10621 ggcaagggct tcctgggggt cagagctggc ctccttgagt gtgtgactta gaccaacact
    10681 gcatattcta cttgcaacta cagatttcct acttcaaaag tcaaaggtat ttaacagtaa
    10741 acactttgtg ggatgagggc gggtttccta ataagccctg tccccagatg tactgctttt
    10801 tcagatagtt ctacaaagta gagcgagaca tatgctagga aagccgtacc aatctggcaa
    10861 ggtcagggac acgtgactaa tgacgagcgg gagtgcatca catgaacatg gagttcattg
    10921 tcttgagcag ttctggactg agcatcgaca gttctggagc ccttctatct cataccatga
    10981 agttaattac gaactgaatg ttagtctaac ctgaaaccac ccaatgatcg tcccagcctc
    11041 tgagacctca ggaaacgaga ctgataacag ggaagagcct cgccataatt ttgcctttca
    11101 aatttgcaaa agaaaatgct tttgtttgct atgcctggca tttgaaataa ccaggatcaa
    11161 agtttagtag aaatcatttt gtaaaaataa tgctttagac atacttacat tcaaaattca
    11221 gatttctatt cttcctgagg tccttattag ctccatggaa tccaaaaata tactatggaa
    11281 aaaggttgtc tttttattgc aaaagatttc tccacattca aggagccaga aactttagaa
    11341 caccaacagt tttactttta caaaccccta taaatcctgc ccctgacttc aatccgatca
    11401 aaacagaact gcagaatctt tattttacag tctttctaag tgaactgagc agacaccaca
    11461 tttaagagtc aatatttatt ttgattaata ttttacaaaa ttttgacagg tttaaattat
    11521 gtaaggagga ctgatttatt aaacacttta caagtttttc tttccacaga gtaacacagt
    11581 aagtagctga ataatacttg gcacagttat aaataaagaa aatataaaac aaaaacattc
    11641 atagcttaag taggatgctt ctttatgcct agtatttaaa ataaactcct acaaaaccaa
    11701 caaaaatatg aatgaaaaaa ctcccaagaa cttcgtccct agctcgtaaa ggaatccaac
    11761 tgacagtgag aactccctcc aaggaacctt caaacacagc ctgagaactg gatttatccg
    11821 gccctgcaca caaggcctgc ggagtaagga aacatggcgc acagtagcct ccagaccatc
    11881 tgacaatcct gttccagact cgcggagacc caaacaagcc gtcagtctgg aacgcatctc
    11941 cttgccaata tcttccgtgg agttccacat ctacaggggt tgcctgcttt acggagaagc
    12001 atctcttgca ctgaaaggat actgtaccac aaagaaagaa catggtaaca acattcttac
    12061 tcaaactgag aaaaccatcg tactctaact gttgataaaa atgtatctga taaaaattgc
    12121 aactgagtct ccatgtgaag tgactcgaaa tagaaaatta aaacaaatcc tagcatttta
    12181 taaactttga atttaaaaat tacctaaaat ttctccccat taatagccaa aactagccga
    12241 catttgtaca gttgaccaat ttttttacat tctatgacaa aatggtacaa tgctattacc
    12301 accccacacc catctcttcc cttttcaaaa ataaagtaca tatgccaatt ctaatattaa
    12361 aaccctcaag tacaatgagg gtattttgtg gaggtgactt acggtcacag agattactat
    12421 aaaatggtag tacttggaaa cactgagcat caactatgta catgtccgct gctcctcctc
    12481 ctaaggcagg gcgggtggac cactagattg ctttaatacc tgtagccagc aatttacagg
    12541 gaatcctaag tttgcctagc actggacatg cagtgaactt gcaggggcga gtgcctatta
    12601 acagcggtat ggaacgtcac gagaagctca ttcatatctc cctttgcttt ttggttggac
    12661 aaaatgaact gagcagctgt aaaaacacta acacgtctag acacagagaa aactatttga
    12721 taagttattt atatacagat acacagtctt tacactggtc taggatgtca gtctgacccc
    12781 gcccccagtc cctcccacag gttgcgaacc acagtttgga tgagggctaa ccagagtctc
    12841 cctgtaaaca atattcatgg aagcagtggc aagtcgaccc tgagggtgtg gaaacgctgc
    12901 aacacaacgt gatcagtgca tcacatttaa cagtaagatc tgctgagaag gcttcagaaa
    12961 gggttaccaa tgtcaaaaca tactcaatat tggagaaatt aaaacaaccc tggggtgtaa
    13021 attccacttt ctgatagatc attaaaacaa gaatattcca ttagcttttc agttattcac
    13081 atgtgctatg tacagaggtc atgccccatc tttagcctag acgccattat tttgtctaca
    13141 atttatttct cagctctgaa tttaaaaagc atatccactt atctctatta ataaagaatt
    13201 ttgcattagc ttattttttt ttgcttcttg aaaaaaatcc atcttaactc ttttaaaaat
    13261 tgggctgtct tgcacatgaa attaaatcct aatttatgct tcactggaca gtatctgcta
    13321 gtatacattc tatgcagtgt atcatgcact tagtagggct ttaaatttca acaaaatagt
    13381 atgacttagt atactcatgc aagtgcttta ttttcagacc gtgagacccc agagcaaggt
    13441 tactagaaat tatctcactg aactagaaat aagccagcat ccccagagcc ggcggctaca
    13501 caagagcgag ccgcttccat gaacattatt ccccgtgcct ttggggacac agtacagtag
    13561 acctctaata aaaccacatg attctcatca cagtatttac atgtacatct aaaatgtaca
    13621 taatactagt catcaattta agacagtttt cttggaatta aatgcaacac tcttaagtac
    13681 tgcataatac aatgtagccg tataacataa atttactttt agttataacg acatttatcc
    13741 tcggtatgcc atctctagta acagaataag ttcacttttt catgaaaaat gttatgagaa
    13801 caaaaccaaa aagtcgagta tgatgtgaat tcagacatta aaaaccatga aaattcattt
    13861 gatgatcttc tcttcctagg cgtggcagtg tgttctgtct atgtttgctt ttgttaataa
    13921 aagtactgaa caaggctgtc gagttcacgg ttagccctac atatagcatg ctattgttcc
    13981 aaggacgtgg attcgaggga gagagagaca gggacagacg caatcctcag gcaccactca
    14041 gtaacactga gttcctggca tccggacgct gctgctacac aagctggtac ccagatcctg
    14101 atatttggtc atattctata gtgtactgcc tggtccagtc cacgataaca ggacgaaaga
    14161 ggccacagca tcgaaattca aagaagtcta taatattcct tacacatcca tggctaaagt
    14221 cagaccccaa aagaaaggag aattcagtta gtatgagtga cttaataact tctatatact
    14281 tttcaagttt aaattttttc taagcttaaa aactcaggat gcttgcaaga aagaagtaac
    14341 ttatcactgt aaaatgaata tccacagctc taccagcaag tattttcttc tttttaagat
    14401 attacacaat tatactttag agtgacccaa ctgctccact tcagtgtgta tatattattt
    14461 gcttctgaga tatctagacc cagctttctt gtacaaagtg gttgattcga ggctgctaac
    14521 aaatcgagca gtgtggtttt caagaggaag caaaaagcct ctccacccag gcctggaatg
    14581 tttccaccca atgtcgagca gtgtggtttt gcaagaggaa gcaaaaagcc tctccaccca
    14641 ggcctggaat gtttccaccc aatgtcgagc aaaccccgcc cagcgtcttg tcattggcga
    14701 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
    14761 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
    14821 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
    14881 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
    14941 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
    15001 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
    15061 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
    15121 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
    15181 ctcctcttcc tcatctccgg gcctttcgac ctgcaggtcc tcgccatgga tcctgatgat
    15241 gttgttgatt cttctaaatc ttttgtgatg gaaaactttt cttcgtacca cgggactaaa
    15301 cctggttatg tagattccat tcaaaaaggt atacaaaagc caaaatctgg tacacaagga
    15361 aattatgacg atgattggaa agggttttat agtaccgaca ataaatacga cgctgcggga
    15421 tactctgtag ataatgaaaa cccgctctct ggaaaagctg gaggcgtggt caaagtgacg
    15481 tatccaggac tgacgaaggt tctcgcacta aaagtggata atgccgaaac tattaagaaa
    15541 gagttaggtt taagtctcac tgaaccgttg atggagcaag tcggaacgga agagtttatc
    15601 aaaaggttcg gtgatggtgc ttcgcgtgta gtgctcagcc ttcccttcgc tgaggggagt
    15661 tctagcgttg aatatattaa taactgggaa caggcgaaag cgttaagcgt agaacttgag
    15721 attaattttg aaacccgtgg aaaacgtggc caagatgcga tgtatgagta tatggctcaa
    15781 gcctgtgcag gaaatcgtgt caggcgatct ctttgtgaag gaaccttact tctgtggtgt
    15841 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
    15901 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
    15961 tggaactgat gaatgggagc agtggtggaa tgcagatcct agagctcgct gatcagcctc
    16021 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
    16081 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
    16141 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
    16201 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
    16261 aagaaccagc tggggctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg
    16321 gttaattgcg cgcacgtttg cccgggagat gggggaggct aactgaaaca cggaaggaga
    16381 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
    16441 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
    16501 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
    16561 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
    16621 gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg
    16681 gggttattat tttgggcgtt gcgtggggtc aggtccacga cccaagcttg gctgcaggtc
    16741 gagctcgcga aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
    16801 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
    16861 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
    16921 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
    16981 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
    17041 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    17101 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
    17161 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    17221 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    17281 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    17341 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    17401 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    17461 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    17521 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    17581 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
    17641 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    17701 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    17761 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    17821 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    17881 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
    17941 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    18001 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    18061 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    18121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    18181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    18241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    18301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    18361 ccttaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa
    18421 acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc aactctttga
    18481 accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca gtttagcctt
    18541 aaccggcgca tgacttcaag actaactcct ctaaatcaat taccagtggc tgctgccagt
    18601 ggtgcttttg catgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag
    18661 cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg
    18721 gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt
    18781 aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa acgcctggta
    18841 tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt
    18901 gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta
    18961 agtatcttcc tggcatcttc caggaaatct ccgccccgtt cgtaagccat ttccgctcgc
    19021 cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tatcctgtat
    19081 cacatattct gctgacgcac cggtgcagcc ttttttctcc tgccacatga agcacttcac
    19141 tgacaccctc atcagtgcca acatagtaag ccagtataca ctccgctagc aacctggctt
    19201 atcgaaatta atacgactca ctatagggag accggcagat ccggcgcgcc tgcaggcgcg
    19261 ccactagtta attatttaaa tcgatgcgac gctagcggcc gcgtttgctt ttgtttcgtg
    19321 ttggtcacca cggccgagtt tcctcgggac cccgggcagc tgctttacat cccaaagacc
    19381 tatctgctcg gccggcctcg gaacgcgagc ctgcccggaa gatccccggg taccgagctc
    19441 gaattcatcg tcaccatcac ctcgaatcaa caagtttgta caaaaaagca ggct
//
