LOCUS       sequence-317916-        2525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-tagged version of Dpp (Decapentaplegic) under control of UAS and
            LexO enhancers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Harmansa S, Hamaratoglu F, Affolter M, Caussinus E
  TITLE     Dpp spreading is required for medial but not for lateral wing disc 
            growth.
  JOURNAL   Nature. 2015 Nov 19;527(7578):317-22. doi: 10.1038/nature15712. Epub
            2015 Nov 9.
  PUBMED    26550827
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature15712"; journalName: "Nature"; date: "2015-11-19- 
            19"; volume: "527"; issue: "7578"; pages: "317-22"
COMMENT     Sequence Label: pUASTLOTattB_eGFP::Dpp
FEATURES             Location/Qualifiers
     source          1..2525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1482..1484,1485..1487,1488..2198)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1482..1484,1485..1487,1488..2198)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1527..1548)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1788..1807)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2135..2156
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaattcagtt gcaagcgacc atgcgcgcat ggcttctact cctcgcagtg ctggcgactt
       61 ttcaaacgat tgttcgagtt gctagcaccg aggatatatc ccagagattc atcgccgcca
      121 tagcgcccgt tgccgctcat attccgctag catcagcatc aggatcagga tcaggacgat
      181 ctggatctag atcgggagga gcctcgacca gcacagcatt agcaaaagca tttaatccat
      241 tcagcgagcc cgcctcgttc agtgatagtg ataaaagcca tcggagtaaa acaaacaaaa
      301 aacctagcaa aagtgacgcg aaccgacagt tcaacgaagt gcataaacca agaacagacc
      361 aattagaaaa ttccaaaaat atgtctaaac aattagttaa taaacccaac cacaacaaaa
      421 tggctgtcaa ggagcagagg agccaccaca agaagagcca ccaccatcgc agccaccagc
      481 caaagcaggc cagtgcatcc acagaatctc atcaatcctc gtcgattgaa tcaatcttcg
      541 tggaggagcc gacgctggtg ctcgaccgcg aggtggcctc catcaacgtg cccgccaacg
      601 ccaaggccat catcgccgag cagggcccgt ccacctacag caaggaggcg ctcatcaagg
      661 acaagctgaa gccagacccc tccactctag tcgagatcga gaagagcctg ctctcgctgt
      721 tcaacatgaa gcggccgccc aagatcgacc gctccaagat catcatcccc gagccgatga
      781 agaagctcta cgccgagatc atgggccacg agctcgactc ggtcaacatc cccaagccgg
      841 gtctgctgac caagtcggcc aacacagtgc gaagttttac acacaaagat agtaaaatcg
      901 acgatcgatt tccgcaccac catcggtttc ggctgcactt cgacgtgaag agcattcccg
      961 ccgacgagaa gctgaaggcg gcggagctgc agctgacccg ggacgcactc agtcaacagg
     1021 tggtggccag cagatcgtcg gcgaatcgga cgcgctacca ggtgcttgtc tacgacatca
     1081 cgcgcgtcgg ggtgcgtggt cagcgggagc cgagctatct gctgttggac accaagacgg
     1141 tccggcttaa cagcacggac acggtgagcc tcgatgtcca gccggccgtg gaccggtggc
     1201 tggcgagtcc gcagcgcaac tacggactgc tggtggaggt gcggacggtc cgctccctga
     1261 agccggcccc acaccatcac gtacgcctgc gccgcagcgc agacgaggcg cacgagcggt
     1321 ggcagcacaa gcagccgctc ctgttcacct acacggacga cgggcggcac aaggcgcgct
     1381 ccattcggga cgtgtctggc ggagagggcg gtggcaaggg cggccggaac aagcggcagc
     1441 cgagacggcc tacgaggcgc aagaaccacg acgacctagg gatggtgagc aagggcgagg
     1501 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1561 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1621 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1681 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1741 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1801 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1861 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1921 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1981 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2041 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2101 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2161 ccgccgggat cactctcggc atggacgagc tgtacaaggg cctagggacc tgccggcggc
     2221 actcgctgta cgtggacttc tcggacgtgg gctgggacga ctggattgtg gcgcctctgg
     2281 gctacgatgc gtactactgc cacgggaagt gccccttccc gctggccgac cacttcaact
     2341 cgaccaatca cgccgtggtg cagaccctgg tcaacaatat gaatcccggc aaggtgccga
     2401 aggcgtgctg cgtgcccacg caactggaca gcgtggccat gctctatctc aacgaccaaa
     2461 gtacggtggt gctgaagaac taccaggaga tgaccgtggt gggctgtggc tgtcgatagt
     2521 ctaga
//
