LOCUS       sequence-317921-        4869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of intC-ZF1-NLS-HA under the ZF6x6-C promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJM572 ZF6x6-C intC-ZF1-NLS-HA in TUPV2
FEATURES             Location/Qualifiers
     source          1..4869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             805..825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             838..864
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(912..929)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    918..1142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1911..2339
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1998..2017)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2208..2229
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2362..2558
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2409..2544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2471..2490
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    2678..2799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2715..2734)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2769..2788
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2848..2864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2848..2864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2861..2883)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2872..2888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2896..2902,2903..2920,2921..2926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2941..2962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3079..3096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3250..3838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3330..3349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4009..4800,4801..4869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4632..4651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacgtcgacg gatcgggaga tcgaagacgg gcaaaaccga tgttgacgga
      181 ctaatcctga caagatctgc gaggtgtagg ggacgcgagt gtaggggtga gctgtgtagg
      241 gggactaagt gtaggggacg cgagtgtagg ggtgagctgt gtaggggaac gctctctaga
      301 gggtatataa tgggggccac tagtctacta ccagagctca tcgctagcgc caccatgatg
      361 ctgaagaaga tcctgaagat cgaggagctg gacgagagag agctgatcga catcgaggtg
      421 agcggcaacc acctgttcta cgccaacgac atcctgaccc acaacagcgg atccggtggt
      481 ggcgggtctg gaggaggagg tagtggtgga ggtggctctg gtaccgctag acccggggag
      541 cgccccttcc agtgtcgcat ttgcatgcgg aacttttcgc gccaggacag gcttgacagg
      601 catacccgta ctcataccgg tgaaaaaccg tttcagtgtc ggatctgtat gcgaaatttc
      661 tcccagaagg agcacttggc ggggcatcta cgtacgcaca ccggcgagaa gccattccaa
      721 tgccgaatat gcatgcgcaa cttcagtcgc cgcgacaacc tgaaccggca cctaaaaacc
      781 cacctgagag aattcggctc tggccctaag aagaagcgca aagttggctc tggcatgtac
      841 ccatacgacg taccagatta cgcttaggcg gccgctcgag tctagagggc ccgtttaaac
      901 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      961 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     1021 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     1081 cagcaagggg gaggattggg aagtcaatag caggcatgct ggggatgcgg tgggctctat
     1141 ggcttctgag gcaggtcgaa gaactatttc ctggctgtta cgcgagcgca tcgcgataag
     1201 ctctagaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
     1261 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
     1321 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     1381 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
     1441 tttaggctga aagagagatt tagaatgaca gaatcataga acggcctgcg tacggatggg
     1501 gctctagctc tagagcttga tatcgaattc ctgcagcccc gctagaggga cagccccccc
     1561 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
     1621 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
     1681 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
     1741 ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt
     1801 agaatgacag aatcatagaa cggcctgggc gcgcctggtg gcgaacgatc aacagacggg
     1861 agaggactaa agtcttcgct gacagcgacc ggactctagg gggtatcccc acgcgccctg
     1921 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1981 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     2041 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     2101 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     2161 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     2221 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     2281 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     2341 attctgtgga atgtgtgtca gttaggctca attagtcagc aaccatagtc ccgcccctaa
     2401 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2461 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     2521 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     2581 ccattttcgg aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     2641 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     2701 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2761 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     2821 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     2881 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2941 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3001 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3061 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3121 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3181 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3421 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3661 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4141 gataccgcga gatccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4861 aatactcat
//
