LOCUS       sequence-31793-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector withTRE promoter driving mouse G beta 2 miR30-based 
            shRNA with GFP coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_TGmiR_Gb2-K
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           45..166
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           194..208
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           236..362
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(395..494)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(606..625)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(684..703)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gctgtacaag taaactagtc gactagggat aacagggtaa ttgtttgaat gaggcttcag
       61 tactttacag aatcgttgcc tgcacatctt ggaaacactt gctgggatta cttcttcagg
      121 ttaacccaac agaaggctcg agaaggtata ttgctgttga cagtgagcgc gctcatgtat
      181 tcccacgaca atagtgaagc cacagatgta ttgtcgtggg aatacatgag catgcctact
      241 gcctcggaat tcaaggggct actttaggag caattatctt gtttactaaa actgaatacc
      301 ttgctatctc tttgatacat ttttacaaag ctgaattaaa atggtataaa ttaaatcact
      361 tttttcaatt cgcggccgca ctcgagatat ctagacccag ctttcttgta caaagttggc
      421 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      481 aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc tctgatgtta
      541 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
      601 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
      661 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
      721 tgcgacaatc tatcgcttgt at
//
