LOCUS       sequence-31794-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector with TRE promoter driving GFP expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_TRE_GFP
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(101..125)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(122..160)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(193..211,212..234,235..253,254..276,277..295,296..318,
                     319..337,338..360,361..379,380..402,403..421,422..444,
                     445..463)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    complement(502..601)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(620..643)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(651..678)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(770..851)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      810..852
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgccttgctc accatggtgg actagttcgg ggccgcggag gctggatcgg tcccggtgtc
       61 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
      121 gagctctgct tatataggcc tcccaccgta cacgcctacc tcgacccggg taccgagctc
      181 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta
      241 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt
      301 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt
      361 tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc actgataggg
      421 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa aggatccagt
      481 cgactgaatt ggttccttta aagcctgctt ttttgtacaa agttggcatt ataaaaaagc
      541 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
      601 ggggcccgag cttaagtaac taactaacag gaagagtttg tagaaacgca aaaaggccat
      661 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
      721 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
      781 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
      841 cctttcgttt ta
//
