LOCUS       sequence-317951-        9306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018255
FEATURES             Location/Qualifiers
     source          1..9306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1686..1706
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1707..1730
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    1756..1812
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1813..1830)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1813..1829)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1822..1844)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2038..2057)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2157..2179
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2217..2235)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3021..4220
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4451..5599)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(5871..6242)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6275..6384
                     /note="incP origin of transfer"
     rep_origin      6537..7168
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      7631..8219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8120..8139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8373..8390
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8507..8528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8543..8548,8549..8566,8567..8573)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8581..8597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8586..8608
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8605..8621
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8605..8621
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgacgaagaa cgccacctac tcgtactggc
       61 tgaccctgcc cttgctgttc ctcctcgcaa tctacttcat cccgcccgcc aacgccgcga
      121 ccagcgatag cgactggccc aagtaccatc ggctcgacaa cggctggcgc tacagcccgc
      181 tggccgatat caacaaagcc aacgtggaca agctcaaggt cgcctggatc caccagccgg
      241 gcgacatcca gcacggcctg caggccaccc cgatcgtcct ggatggcgtg atctactatg
      301 tggcggccaa taacaacgtc tacgcggtcg acggggagac gctgtggcat tatcagccgg
      361 agctggaccc catcgtgacc cgcgtcttct accaggccgc gagccgcggc gtgaccgtcg
      421 gccacggtat ggtctacctt gggaccctgg atggacgctt tatcgcgctc aaccagaaga
      481 ccggcaagcc ggtctggaag cgtaagatca ccgatctcaa gtcgtgctac ggctgcctct
      541 tctcgtcgcc tccgcagttg gcgggcgagg tcctctacgg tggaacgact ggtggcgacc
      601 agccgcagcg gggcaagatc tatgccgtga acgccctgaa cggccagccg ctgtgggaat
      661 tcgacaccat cagcgacgac cccgagagct ggccgggcga cacgggcaat gtcggcgggg
      721 gcggtgcctg gatgccgggc gtttatgatg agaagacgga cctgatccac atcggcacct
      781 ccaacgcggc gcccgacttc ttcggcgagg tgcgcaaggg cgataacaag tacacggcca
      841 gccttctggc gatcgaggct aagacgggca agctgaagtg gttcttccag gaggtgcccc
      901 acgacgtctg ggactacgac agcgcgtatg agatcgtgac catcgaacac gagaatcgcg
      961 acgtcatctt tcacctgaac aagggcggct tcgtgtacgt tctggacaag aagaccggca
     1021 agcccatcaa catctggccc ctcagccaga cctacaactg ggttgagtcc gtgaacccca
     1081 agaccgggga gctcatcggc cgcaatgaac ccaagctcgg agaggagaag gtcctgtgcc
     1141 cctacctgct cggagcccgg tcgtggaacc acggcgccta ctccccgaag accggactgt
     1201 ggtataccaa cgcaatggag gtctgcaaca agatcatcgc cgccgaacag aacccggaga
     1261 agatcggcat ctccggcctg tatctgggcg tctcgctcct ggaggccgtc gcgccgccga
     1321 accaaaaggc ctctgcccga ctggacgcgc gggacccgat caccggcgag gtccggtggt
     1381 cggtcaacta cccgatcccc ggcctgggca gcgtcctggc gaccgcggga ggcctggtgt
     1441 tcaacggcga cgtgttcggg aaggtgttcg cgtacgacgc ggccaatggc gacgagctct
     1501 ggtcgttcaa caccggatcc ggaatccgct caggtattat cagttatggc aagaacggca
     1561 agcagtatct gctcgtcccg agcggcttcg ggtcgcatgc ccccggcttc atggcctccg
     1621 cgttcccgga ggtgagcggc ctcccgggcg gggcggcgtt gatcgcgttc tcgatcgaca
     1681 aggccgaaaa cctctatttt cagggacatc atcaccatca ccatcaccac tgacagctat
     1741 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
     1801 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     1861 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     1921 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     1981 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     2041 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     2101 gccctgacgg gcttgtatgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     2161 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     2221 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agcacccttt
     2281 ctcggtcctt caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc
     2341 gcgagtccct gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc
     2401 cgcaacagcg gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg
     2461 tcgccggcct gctcctcaag cacggcccca acagtgaagt agctgattgt catcagcgca
     2521 ttgacggcgt ccccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc
     2581 gtttccatct gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag
     2641 gcgagcagcg cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg
     2701 tcggctctcg gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg
     2761 agcagcgccc gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt
     2821 tccgccagtt tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg
     2881 gcacggatca ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac
     2941 ataatatgtc caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga
     3001 ggctggtccg gaggccagac gtgaaaccca acatacccct gatcgtaatt ctgagcactg
     3061 tcgcgctcga cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg
     3121 atctggttca ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt
     3181 tggtgcaatt tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc
     3241 caatcttgct cgtctcgctg gccggcgcca ctgtcgacta cgccatcatg gcgacagcgc
     3301 ctttcctttg ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg
     3361 cggtagccgg cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg
     3421 gcttcatgag cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga
     3481 tgggcggttt ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt
     3541 tcctgacggg ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc
     3601 gggaggctct caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg
     3661 ccctgatggc ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg
     3721 tcattttcgg cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg
     3781 catttggcat tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc
     3841 tcggcgaaag gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc
     3901 ttgccttcgc gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg
     3961 gcatcggaat gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg
     4021 ggcagctgca aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc
     4081 tcttcacggc gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag
     4141 gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag
     4201 ggcaacgagc cgatcgctga tcgtggaaac gataggccta tgccatgcgg gtcaaggcga
     4261 cttccggcaa gctatacgcg ccctagaatt gtcaatttta atcctctgtt tatcggcagt
     4321 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
     4381 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
     4441 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
     4501 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
     4561 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
     4621 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
     4681 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
     4741 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
     4801 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
     4861 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
     4921 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
     4981 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
     5041 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
     5101 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
     5161 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
     5221 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
     5281 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
     5341 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
     5401 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
     5461 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
     5521 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
     5581 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
     5641 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
     5701 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
     5761 tcttgccctg cacgaatacc agctccgcga agtcgctctt cttgatggag cgcatgggga
     5821 cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct
     5881 gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc
     5941 ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg
     6001 agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg
     6061 cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg
     6121 taggcctaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct
     6181 ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc
     6241 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc
     6301 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
     6361 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
     6421 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc
     6481 gctataatga ccccgaagca gggttatgca gcggaaaaga tccgtcgacc ctttccgacg
     6541 ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
     6601 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc
     6661 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
     6721 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
     6781 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
     6841 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
     6901 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
     6961 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
     7021 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
     7081 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
     7141 cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg
     7201 cccctcggcc gcgaacggcc tcaccccaaa aatggcagcc aagctgacca cttctgcgct
     7261 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7321 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7381 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7441 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7501 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7561 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7621 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7681 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7741 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7801 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7861 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7921 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7981 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8041 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8101 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8161 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8221 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8281 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8341 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8401 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8461 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8521 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8581 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctag
     8641 cttcccgctt ggtcgggccg cttcgcgagg gcccgttgac gacaacggtg cgatgggtcc
     8701 cggccccggt caagacgatg ccaatacgtt gcgacactac gccttggcac ttttagaatt
     8761 gccttatcgt cctgataaga aatgtccgac cagctaaaga catcgcgtcc aatcaaagcc
     8821 tagaaaatat aggcgaaggg acgctaataa gtctttcata agaccgcgca aatctaaaaa
     8881 tatccttaga ttcacgatgc ggcacttcgg atgacttccg agcgagcctg gaacctcaga
     8941 aaaacgtctg agagataccg cggatctcac acaggaaatt tcgcaacgca aaatgctgaa
     9001 tttagcaggc aagtttcctg ataaagggcc ctgtgagtgc gacattcgac gcgcgaaagc
     9061 gagttgacgg cgcccgaaat tttcttagag ttcgttcaag cttcggtttc ggggagccgc
     9121 taagggatgc ggacccagcc gggtaagtcg ccgggaacta tggaataatc ctaatttctg
     9181 tgcggcaggc gcgagctgcc ggagcgaata tcatccctgt cgcaaacagc aggtcaatca
     9241 acggtgggta gcgggctgct atccagcggc ggataagact tgtcggagga atcctctaga
     9301 aataat
//
