LOCUS       sequence-31796-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Navarro F, Gutman D, Meire E, Caceres M, Rigoutsos I, Bentwich Z, 
            Lieberman J
  TITLE     miR-34a contributes to megakaryocytic differentiation of K562 cells 
            independently of p53.
  JOURNAL   Blood. 2009 Sep 3. 114(10):2181-92.
  PUBMED    19584398
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2009
            Sep 3. 114(10):2181-92."
COMMENT     Sequence Label: pSIREN-RetroQ-MYB-shRNA
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..129
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        262..761
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(297..320)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     694..713
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(782..801)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
       61 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      121 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      181 aggatccgct tccagaagaa cagtcattca agagatgact gttcttctgg aagctttttt
      241 gaattctttt ttaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
      301 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
      361 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
      421 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
      481 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
      541 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
      601 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
      661 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
      721 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
      781 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgt
//
