LOCUS       sequence-317967-       18327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Positive control for germline GFP expression from simple array in C.
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 18327)
  AUTHORS   Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, 
            Frokjaer-Jensen C
  TITLE     Engineering rules that minimize germline silencing of transgenes in 
            simple extrachromosomal arrays in C. elegans.
  JOURNAL   Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.
  PUBMED    33298957
REFERENCE   2  (bases 1 to 18327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-12-9"; volume: "11(1):6300. doi"; pages: " 
            10.1038/s41467-020-19898-0"
COMMENT     Sequence Label: pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1
            ...
FEATURES             Location/Qualifiers
     source          1..18327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(6523..6547)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6576..6604)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7352..7372
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(8404..8424)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8432..8449)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8432..8448)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10275..10297
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10335..10353)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10421..10525
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10526..10594,10595..11386)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10744..10763)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11557..12145
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12046..12065
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13781..13792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    15858..15878
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    16874..16898
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtagccag tcaaccactt tctaaaatat caaatttgaa tttttcaagc atttttacat
       61 tgaaaaatct aatttttcga gtgaaaccac ttgaaaatct gaaaattgaa ttaatttctg
      121 aactttagag attttttgtc aaaattttag ataaagggat ttttaaacaa aaaattgatt
      181 ttttaatcga aaaatactgg gatttatggg ttttttaaaa gaaaaacggg ggtttgaatg
      241 aaaaatcgcc tgaaatctta gaaaattaat taaaaaacta tgatttaatt ccaaaaatta
      301 ccgaaaatat caaattttcc atttttaaac cctaaattct ttcagaaagt tatcccgaat
      361 cggcagtttt ctcaccggac gctaattatc ttgtttctgg ctcaaaagac ggatttatcg
      421 aagtttggaa ttatatgaat ggaaaattga gaaaggattt gaaatatcag gtagattttt
      481 tagaattttt gggtttttga agtagaaaat cataaaaatc tagggttttt atgaattgtt
      541 ttgaagaaaa attgcaaaaa ttccacaaaa tggaagaaaa ataactttgg aagcgcattt
      601 ttcgcaaaaa aaccgaaatt tttgcgtaaa atttcaaaat tgcaataaaa attccacaaa
      661 catcaaattt cttaaatttt ttataaaaaa ttggatggaa acactcttga atttagaaaa
      721 aaaatcagtt tttctcatct aaaacttcaa aatttcggtg taatcccatt aaaattgcca
      781 caaaattcgg aaatttcacc tgaaatagag tgaataattt aaaatgttca gaaattcata
      841 ttttgtcatt ttaaaagcat taaaacaaat caaaaatcta ttttttttgg ttggaaaagt
      901 tcaaaattct ggagaaatta cattaaaatt ttcgtaaaat atggtaaata aacgaaaatg
      961 ttgagaaatt aaagaaaagt tacaattttt agcttaaaaa ttcaacattt tgaggaaatg
     1021 ccacctaaaa aagtgactaa tcgaaaatgt tgagaaatta aaattgccac catttattta
     1081 tataaactac tctaaaatta caattttcat gttaaaaatt aataaaaaat ctactttttc
     1141 caaactacag taaccttacc gtatacctac agtacctgaa cattgccccc caccagctcc
     1201 caacccaata cctcctcaaa aacttacacc tcaatttttc ataaactaca gtaaccctac
     1261 caaaaaagca caaaaaaaaa tctacattca ttttccaaca attttcaata ttttcaggcc
     1321 caagataatc taatgatgat ggatgcagca gtgagatgta tatcatttag tcgtgattct
     1381 gaaatgctcg ccacaggctc aattgacgga aaaattaaag tttggaaagt ggaaactggt
     1441 gattgtcttc gaagatttga tagagctcac acgaaaggag tttgtgctgt tcgattctca
     1501 aaagataatt cacatatttt gtcgggtgga aatgatcatg ttgtgaggta cgactacctg
     1561 cctgcctacc gcctaaattt tttgtgaagt ttcttcaaaa aatccagaaa aaaaaacaat
     1621 tttcatacga ttttttccct taaaattgtg aattttcatg ctttttagcc ccaaaagtca
     1681 ttatttgaga aaaaatttca tacaaaaaag ttttgagaaa tacacaattt tttaaatgta
     1741 attttcaaat tttcaatttt caactagaaa attcacaaaa cttgtaaatt ttggaccaaa
     1801 acatttatac aattactttt tttgaatcta ataactacaa taactacaaa ttttgttcag
     1861 agttcatgga atgaagagcg gaaaatgttt gaaggaaatg cgtgggcact cgagttatat
     1921 tacagatgtc agatattccg atgagggaaa tcatattgtg agtttttgaa aagtgcagtt
     1981 tgtagtctaa ttttcattta ttttcttttt aaaaaacgca tcaattttta aatattttgg
     2041 gacaaaatcc gaaaactgta ctaatttgta gtttgtaaaa ttaaaaaaaa aacgcaaaaa
     2101 aatgttttca aaaatgctta gaataaaaat aaaatttcta aaaaattgaa caaaataaac
     2161 atttaaaaat tcaaaaagtt tgaaaaaatg caaagttttt taatgcaaaa aatatttaat
     2221 gtttaaacaa atttaaaaaa tgttgtaaga atatgtttag agacagtttt taaatgttaa
     2281 attgcaaaaa aaaaaagcaa aaaaaaattt gaaaaatgta acagaaaatt ttaattaaaa
     2341 aagtcgtgtt tttttcaaac ctgcaattct ttgtaatttt atttataaag agaaacaaat
     2401 caaattgtat attacgaaaa acgatactaa aattcgaaaa tttgcggttt tttgcgtaaa
     2461 aaatacggtt cctgaaaatg ggtgtgcacc tttaaggagt actgtagttc taaactttca
     2521 ttgctgcaaa atttatatct attttttgaa gttttacaat aaaaataaca attttcatta
     2581 aataactaca attaaacagc taaaaacata taagaaatcg cgagaaaatc gggaaaaaat
     2641 tgtagtttgt agtatgttag ccgcaatcaa atgcatgtta tctctgtatt tcttttctca
     2701 cacaaatccc acatatttct atgtttacac acgtcatttt gtgcactttt tctgacgttt
     2761 cccctccata ttgattttgc tttttactgt ttagaaacgt tgcagaaatc cggaaaaatt
     2821 gatttttttt ctatatatat atattttttt ccaagtttca aatttaaatt tcagaaaaaa
     2881 aatttagact taaaaaaatt aaaaatccca aataatttcc agatctcatg ttcaacagat
     2941 ggttcaattc gtgtttggca tggaaaatcg ggtgaatgcc tttcaacatt ccgtgttgga
     3001 agtgaagatt atccaatatt aaatgtgatt ccaattccga aaagtgatcc accacaaatg
     3061 attgtatgca atcgatccaa taccctctac gttgttaata tttctggaca ggtaacatga
     3121 ttttgaaaaa aagtttaaga actgaaaaat ggaataaaaa tatttaagag catttttaaa
     3181 tgtaaaatta acaaaaaaag cgcctaagaa tgtttccaaa acagtaaaaa aaaggtttaa
     3241 aaaaatgcaa aaaaaaattt aagatgcttt taattacaca aaaaatgaaa gtaaaaaaaa
     3301 ttaaaatatt gaaaaatgta tatttgtttg aaaaggtaca tttttcaatg caaaaaatgt
     3361 ttctaaaaat taaaaaaata tgtttaaatt gtgtaaagtg taaattaaaa aataaaatta
     3421 tgaaaaagta ttgtttcata ctttttctaa attttggttg aaaaatttaa aattccagct
     3481 ttttattcac attatgtgtt ctaaaaatct ttccccaatt ttcgggaccg ttttccagta
     3541 tatttccatt tttcaaaaag atttttaact gaaattcatg ttttcaatgc taaaaatcaa
     3601 taaaaaaaat ttcaggtggt tcgtacgatg acgtcaggaa agcgtgagaa aggagacttc
     3661 atcaactgca ttttatcgcc gaaaggttaa ttttgacccg aaaattgaaa aaaaaaaccg
     3721 atttcaaaat tctaattttc ccattttttt ctttaaaata atttctcctc tggaaaggct
     3781 cgtagagcag aaaaagaatt aaattagaaa cggatttttg ttcaaatttc ccccgttttc
     3841 cccttcaaaa cttgcaattc agtttcttac aggagaatgg gcatacgcaa ttgcagagga
     3901 cggtgtcatg tactgtttta tggtattatc gggtaccctg gaaactactc ttcccgtaag
     3961 tttagcctct ttttgaactc aaattttagt ttaaaaagcg agaaaaagta caaggaaaat
     4021 gagaaatcaa gtgcatgaat tgataagaat agtatggtga gggtgtggtt gatgactaga
     4081 aaaaatcgtt tttttttaat catacacaga ttttcttaaa ataaaaacat caaaaatgtg
     4141 tcggactaat tttgtaaaat aaaatttaat cgaaaaatga ggtaaagttg aattttttga
     4201 ctccaaaaac ctctatttct acgctcaatt ttgtaaaaaa aaattcaggg taatttttat
     4261 ttcaatgtct acaaattttg ggggtattgc tactgaaaaa gtaccgaaaa attatttttt
     4321 tcgcaaaaaa ccgttatttc tgaaaaaatt ctgaaattta ttcttcaaaa tgttaaaaat
     4381 acgcagaata tttcagtacc aaaaaactaa aaaaaaaaac aaaatatcac aaaaaaacga
     4441 aataaatcga attgcgaatt tccccaactc tcgttagatt ccctatttga attccttcca
     4501 aaatgaaaac tccccgtcca atcagcgtct tcgaacactc cgcccactca ttctgattgg
     4561 ttcaataatg gacggctata gaaaatctat agaaaatcac ttgatttatc tgaaaccaac
     4621 attaatttca gctgaaaaat tagagaaagg gctcaaaatt ggcctgaaag ttgccaattt
     4681 ttattaaaaa aattggcaac tttcaggcaa atttttatat attttaaaat tctaattagt
     4741 cctcatttcg atattccacg tttccgaatt ttgatatttt tttttcaaaa tatatattcc
     4801 tcattaaaaa tgttctagtt ttttttacaa catccctctt gcctccaaaa tttttcgatt
     4861 ttctcccatt cctccctcac tcggaagaat gttcttcgac tgacgtagtg gtagtagcag
     4921 cccataacac atacgtagat taattttata gatagtatag ggttttccta caccgatatt
     4981 cattccattg ttcacttttc gaattgtttc atattgcgat tttaaaggcg catggcttga
     5041 aaagtatgaa gtttagaaat tttacagaaa aaacgaaaaa aaaatccgaa aaaaccagaa
     5101 tttcctatct cctccctcac taacctcaat aaattcccag tttgaattcc ctccatattg
     5161 tgaactgcaa aaccaatcag cgatttgctc tgcccacttt tcaaccaatc agataaagtg
     5221 ggcggggttc gaagtctctg ggggcgtgtt acggagctac ctatttcatc atgctcttcc
     5281 cagacgtggg caacggttgc cattcggcaa ttttttggtc gacaagttcg gcaaatctgc
     5341 aaattgccgg tttgtcgatt tgccggaaac agccgcccac ccctggttcc tccaacattt
     5401 ttcattaaaa atttgtgtaa aacttcagaa aatctctcga ctttccttta aaaagaaaca
     5461 ctaatttcag ctgaaacact cgaaaatggc ctaaaagttg gaaattgccg attttcagac
     5521 catttttgag cattttctct aagatttttg tagtttgtag tctttctgcc aatttgttta
     5581 attcagtctt cccctctctc caattaaaca attcccgttc acatattttt ttcaatatga
     5641 ccgcactcgg tacacttttc tgtctgcatc tctactctct ctctctctct ctcacggaaa
     5701 attcattttt tttgttgttt ttctcttccg tggtccgtct agaagatgct aggaatacta
     5761 aatatatata tttcgtcaat tttttttccg cccgcccgtc tctcgcccaa cgtgctaggc
     5821 aaccggcttt tttttgttga aaattttgaa gatttcgatg aaaaatgtag attttttttc
     5881 gggtttctga gctgaaaatc actgataaag ctcacaactt tatcagaaaa tgtttgattt
     5941 ttttttcaaa ttttgggtcc cgccacgatc aacctgatga cggatactgt agtcttgaaa
     6001 attaggtttt attgaatttt taggaaaagt ttaaatattc agctgaaatt cgaaaattgc
     6061 agtctgagcc acaatcaatt ttttttcaaa agcgccccat tcttggatct tgcaacgaag
     6121 attttttagg ttactgtaga aacaaaaaat tgtaggttgt agtcatgaat atttttcgac
     6181 tttttcaaaa taaataagtt caaataaaat ggaaattcag cgaaaaaaaa gtaaattttt
     6241 atgagaaaaa tgaagaaaat ttaaaaaaaa acaaaaaaat tttttcgcta taaaaaaaat
     6301 tagaaacgaa tattttcaag aaaattcaat aattttgatt gaaaattgga gaaaaaagtt
     6361 ccagtttttt gctatttttc ttatttttta ttgaaaaaaa aatttaaaaa tcgataattt
     6421 tccaggtgac agaacgtctt ccaattggtt tggctcatca tccacatcag aatctcatcg
     6481 cttcatatgc agaagatgga cttttaaaat tatggaccga taacccagct ttcttgtaca
     6541 aagtggcgag caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     6601 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     6661 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatgggtaa
     6721 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagcca acacttgtca
     6781 ctactttctg ttatggtgtt caatgcttct cgagataccc agatcatatg aaacggcatg
     6841 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     6901 atgacgggaa ctacaagaca cgtaagttta aacagttcgg tactaactaa ccatacatat
     6961 ttaaattttc aggtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     7021 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     7081 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttgtaa
     7141 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagaac ttcaaaatta
     7201 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     7261 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     7321 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     7381 ggattacaca tggcatggat gaactataca aataattttc cccagatttc cccacgattt
     7441 tttttgacat ttttatgaaa attttctcta aaagttaccc ctattttgtt tagactgatc
     7501 tcttatttta tccaatgttt caaatttttg aaattgctcg aaaattccaa ttttcacata
     7561 tttttgttga ttttttcgcc attttcccct gataaattaa cttatttttg caattttttc
     7621 tttcgaaaaa atattggttt ctcatcacca tttatccttt tttttatgga gttaaaaatt
     7681 gttaaaaaaa tttaattttt tttctaattt tagagtcaaa acgggttcga atctagagcg
     7741 tagctgtacg attttttggc gatttttgcg gttttctggt gagttttttt aaagttattt
     7801 ttttgaaaaa aattcttgaa aataaaagga aattgtttta aaaaaaaatt tcaaagaaaa
     7861 aattttgaaa aaattgaaac atttttttta ttttgaccaa aaatattgaa aattaattaa
     7921 catttttttt tggaaagtta atttttaata caaatttttt aaatttattt tacaaaaaaa
     7981 attgaatttc tgtgaaaaat cagttttcca gtacataatg atttgaccaa aaaaaattga
     8041 aaaaaatgat tttttaaaat taaaaatacc ataaaatgct gaaaattcag aaaaataagt
     8101 aaattttaaa aaattctgga aaaacatcta aaaacccccc aaatataata ttttggaccc
     8161 ttttattttc tgctcgacat ctcaaatatc ctccaatttt tcgctttttt gattaattga
     8221 aagcatagtg ggggcaggtg ttataggaca cccttccctc cacttttttg gagcattttt
     8281 caaagctctt ccgagagcag aagcttctca atttttccac cccaaaagtt tgaaattctg
     8341 ccaaaaaatc tcgcatttta acaatttttc gagatgtttc acgtcctttt cattgcgatt
     8401 tcccaacttt attatacata gttgataatt cactggccgt cgttttacac tcgagacgta
     8461 cggtgcgcgc gatgcattcg aagatctgcc cactagtgag tcgtattata agtgcaagta
     8521 agatcagtgt ttgtttcgat atcagtctgt ttcgtaacgg tcttctgtat aactacaaaa
     8581 aagaataaaa aaccgtatca caactgagaa aaaagacaaa acttattgaa cagtgttcct
     8641 ttcaaaaaat tattttgtaa aaaatcgagt ttttctccaa aataacgcac gcaatcaaaa
     8701 acaagaaaac taacgtgttc tcctgggcgt ggaactcctt atcaagatta ccattattgt
     8761 ctgtcagaat tgatatttca attatttcat atctagacaa acccgggtgt cttgcattta
     8821 ttagtactgt gagtttacat acaaacgagg cttactcaga taacatagag tcacctctaa
     8881 ttagcaaaaa cccatttcta attagctatt tcgaccagtt ctaaagtcac cgtaattaga
     8941 aatggttttt gtagcttttc taattagaaa tgaccccgag ataagtgaaa tatggcattt
     9001 tctgggtttt ctagtaagaa atgatcccgc acgtagtgat tccgcgttac aagccaaatt
     9061 caaattaaaa aagtatatta cacaattcaa gttacattat tttcaggaaa caatggcgaa
     9121 gagcaaagca gagattcaaa aggaggaaag agacaacaga tatgaacgaa gacttcttga
     9181 aaagagaaag agacatgagg tgagtaacca tgataataaa taatcaagta agggctatag
     9241 atttataaat tctggagtta acagtttcga aaaaaagtag ttttcatatg tgcgaaggaa
     9301 tatcaaaaat aggggaatag cctaaacgag tgagaaaatt aaatgtttgt tattttttca
     9361 aaaatgtctg cagggccgtt atcctttcaa atgcactcta tcaaacatag atcatatcca
     9421 tcatatctta tccagaattt atatcatatc caagattgag agcgtttcaa ggaaaatctc
     9481 aaacaaccat acaattttgt agatcgacaa caaggcctcc acttcttcat cgatggcctc
     9541 tgcagcacct gcaccaccaa gtttgtattt ttcatattaa tataaagtga gccacgcata
     9601 gtcgttttta ggatttttgc cagatcgatg tcgcgcaaac agactgtttt tttttctttg
     9661 aaagccgtgc gtgacatggg ccacggattg taacgccgcg ctggatggat aaaaatttat
     9721 tcagtttttt agcctttaca aaatatggtt cgactacttt ctttattttt acatttttat
     9781 aggtgctcat tgagctgaga tattttgggt ataatcggaa aatcgctgat tttggtcatt
     9841 tttctgcact aaagattaca caaaaagttc agtatttaga ttcattgttg aaaattgggg
     9901 atgatctaca gatacatatc gccgtgttca aaaacacacg tgctgtgacg tcgtgactgg
     9961 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    10021 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    10081 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
    10141 atggtgcact ctcagtgcaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
    10201 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    10261 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    10321 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
    10381 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    10441 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    10501 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    10561 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    10621 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    10681 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    10741 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    10801 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    10861 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    10921 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    10981 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    11041 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    11101 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    11161 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    11221 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggt cagatggtaa
    11281 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    11341 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    11401 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    11461 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    11521 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    11581 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    11641 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    11701 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    11761 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    11821 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    11881 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    11941 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    12001 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    12061 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    12121 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt
    12181 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    12241 ccgtattacc gcctttgagt gagctgatac cagcggataa caatttcaca gggccctgtc
    12301 ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa tgttgagtca
    12361 gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg aacaattact
    12421 ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt accacagtat
    12481 aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta ttgtatttgt
    12541 gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt ttttaagacg
    12601 agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta gataatcgtg
    12661 aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa acgttcaaaa
    12721 tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc ttctccttct
    12781 cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa aaatacgtat
    12841 tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg gttatactta
    12901 tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc agaactggag
    12961 ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat cgtagtattg
    13021 ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca tttacagtat
    13081 tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca attccttcat
    13141 ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga gagattcatg
    13201 atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata cggccgaaat
    13261 tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga gagattttgt
    13321 tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat acacccgaag
    13381 tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca aaaactctga
    13441 atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac aagagattct
    13501 tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc cttgttaaaa
    13561 tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat gatggtagca
    13621 aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt cgaaaattgt
    13681 taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc actttttgat
    13741 atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct attacagggt
    13801 ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc atcatgctca
    13861 aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct tcttggctca
    13921 tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat actctctcgt
    13981 cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat gacttgcgcc
    14041 cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct tttctccttc
    14101 ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg aagaagaaga
    14161 agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc tcggttaaga
    14221 gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca atcactccac
    14281 caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct ttttcaaatt
    14341 attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt tacaaagtgt
    14401 attttgatta catgtactca aaaaggttga aatttttcat accagttttc cggaaatcca
    14461 tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa caaaatatag
    14521 ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct tttccttctt
    14581 ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg agcaacaacg
    14641 atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa aactagagaa
    14701 accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt ttgttgaact
    14761 tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat cacaacggaa
    14821 tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact tccgggaatc
    14881 actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag gattcttatg
    14941 ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc gtgatctgga
    15001 cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga atgatgagtc
    15061 gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt tgaaactcaa
    15121 aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc atttccgaat
    15181 gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg aattcggatt
    15241 ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc aactctctca
    15301 acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct ctttttcagt
    15361 ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt tcgtcgataa
    15421 caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat ttaatacaaa
    15481 aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga ttaaattcca
    15541 ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg cattcctaag
    15601 cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact cgctaaaaac
    15661 actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat tttcaatttc
    15721 aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac gtaatacgac
    15781 tcacttaagg ccttgactag agggtaccag agctcaccta ggcaggaaca gctatgacca
    15841 tgattacgcc aagctatcaa ctttgtatag aaaagttgaa atttttcagg cgggaagtgg
    15901 cttcggtgcg ttcaacaaac gcgcttttga ctcgctagcc ggatccggct tcagcggttt
    15961 tgacaaaaga gctttcgatt cactggctgg acaaggtttt actggatttg agaaacgcgc
    16021 ctttgataca gtggtacggt tttcggtgga aattccagtt tttcgaattt tcaataattt
    16081 tcagagcacg tcaggctttg acgatttcaa attgtgaaga gctcactgat atttaaaatt
    16141 tggttcaatt caagcacata tttttgaggt aagcaactga ttttaatgtt gttaatcaaa
    16201 tgaaaagtga aatttttgga ataatttttt tttgttttca ggtagctcca acgaacgaca
    16261 cacacaagcc cagtccccca gtattcctga cgacagcccc tgctcacaca ctttttatta
    16321 taattccttt tttttcaaac ttttttttca aattttatac tttaatctca aaaaacctat
    16381 tcatacgaac acaagtaccg gtgtgcgtgt gtgagaataa gaatttattt taaaaaaata
    16441 aagaatgtcg tcctgttttt tcatatttat tgaagaaaat ggagctggaa aaaaatcaat
    16501 tgtaaaaaac agaaaaacgt gctgataaaa tgcattgtac gcaaactgtg gattttgaga
    16561 actaacgtag tcaagcaaaa atctctggaa ttttatcgat ttttaacgga taaactgttg
    16621 ttttttgtaa ctgtgcatga aaaaaaatct caggcaaaaa tattttctga aaaaaaaaga
    16681 gtttaaacac aaatgcaagt ccacaaaaaa ctccaatttg aaattcgctc caaaaacgca
    16741 aaacacgcca agcgctcgtg ctggcgcatt tgccgatcgc ttccaaaaag gctggcgcac
    16801 ttctcgtgtc cgaggatatt cgaattttac acgtcatttt cagccgtttt tcaccgaatt
    16861 ttcgctgaaa atgacaagtt tgtacaaaaa agcaggctcg tcatcgattg agatcgaatc
    16921 ttcagagtaa gtttatctaa aaagtttttc attcaaaatg tgtaaaaatt catttaaaat
    16981 aacccaaaaa tcattaatcc tcgatatttt cagtgtaatc cgtctcatcg aacaattcct
    17041 caaagaatcg aatttgcacc gaactctggc gattttgcag gtattttcct gcatttttca
    17101 actgggaaaa tgaaagaaaa tcgataattt caggaagaaa ccaacgtttc gctcaataca
    17161 gtcgactcaa ttgatggatt ttgtaatgaa attacgtctg gaaattggga taacgtgttg
    17221 aaaaccgttc aatcgttgaa attacccgcc aagaaactta tcgatctgta tgaacatgta
    17281 agatatggga aagaaggaaa aaaccgagat tttacttgaa aaattgaatt tttcgcggga
    17341 ttttcaccaa aaattgttga atattcatta tttcacgctg taaaacaaaa aaaaaaaaaa
    17401 tcaaaaacta cgttgaaatc gcgtttttaa gcgaattttc ttcagaattg ccagatttta
    17461 accccaaatt ttgcagtttt taaataaaat ttcacctttt cggctcaaat tgtagatttt
    17521 cctgaaaatt tagtacaaaa aacaatttcc tcgtaaattt ttcaaatttt caggtaataa
    17581 tcgagcttgt ggagctccgt gagctggcga ccgcccgtct cgtcgcccgt cagactgatc
    17641 caatgatttt gctaaaacaa attgatccgg atcgattcgc ccgcctggaa tctcttatca
    17701 accgcccgta tttcgatgga caagaggttt atggagatgt tagtaaagag aaacgacgct
    17761 ccgtaattgc acaaactctt tcatcagaag ttcatgtagt tgctccgtca agacttttga
    17821 gtttacttgg acaatcactt aaatggcaac ttcatcaagg acttcttcca cctggaactg
    17881 caattgattt atttagagga aaagctgcac agaaagagca aattgaagaa agatatccga
    17941 ctatgatggc tagaagcatt aaattctcaa cgagtacgga ttgaaattgc tttaaaattt
    18001 gaaaaattga ttaaaaagtg cattttttaa gctttgaccg acttaaaatt agatttctga
    18061 gcctattttc tgagaattgg aatttttttc attgtgaaag ttcaaagaat agcgtgaatg
    18121 attagaaaat attgtaaaat ttcaattttt tcctataaaa aaggattttt ttaggaatca
    18181 aaaattgcaa aatgatgccc taaaattcga aaataaatat aaaaattggc ggtttctcaa
    18241 aaatctagaa ttccgacctt aattattaat ttttttacaa tatttttttt tgaaaaaaat
    18301 ccagaaaatt tgaatttcgt agtttgt
//
