LOCUS       sequence-317970-        2129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Frangedakis E, Guzman-Chavez F, Rebmann M, Markel K, Yu Y, Perraki 
            A, Tse SW, Liu Y, Rever J, Sauret-Gueto S, Goffinet B, Schneider H, 
            Haseloff J
  TITLE     Construction of DNA Tools for Hyperexpression in Marchantia 
            Chloroplasts.
  JOURNAL   ACS Synth Biol. 2021 Jul 16;10(7):1651-1666. doi: 
            10.1021/acssynbio.0c00637. Epub 2021 Jun 7.
  PUBMED    34097383
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00637"; journalName: "ACS Synth Biol"; date: 
            "2021-07-16- 16"; volume: "10"; issue: "7"; pages: "1651-1666"
COMMENT     Sequence Label: 5UTR_Mprbcl_chlo
FEATURES             Location/Qualifiers
     source          1..2129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     160..179
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     407..425
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(429..472)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      577..634
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(658..675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(829..1417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(909..928)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1600..1694)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1715..2129,1..245))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
       61 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
      121 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
      181 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
      241 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
      301 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
      361 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      421 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
      481 ggtctctcat taagtccctc cctacaaatc aaataatatt cttgcaagta tgaggtctgc
      541 tcgataaaaa tttttttcta gtaagagacc ctgcagtccg gcaaaaaagg gcaaggtgtc
      601 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
      661 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      721 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      781 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
      841 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      901 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      961 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1021 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1081 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1141 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1201 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1261 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1321 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1381 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1441 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1501 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1561 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
     1621 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1681 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     1741 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1801 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1861 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1921 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1981 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     2041 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     2101 aacaagggtg aacactatcc catatcacc
//
