LOCUS       sequence-317975-        2796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS_mTurqcp_chlo
FEATURES             Location/Qualifiers
     source          1..2796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(546..640)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(661..1320)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1235..1254
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1482..1500
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1504..1547)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      2319..2376
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2400..2417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2571..2796,1..363))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2651..2670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
       61 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      121 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      181 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      241 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      301 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      361 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      421 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      481 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg
      541 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      601 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
      661 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
      721 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      781 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      841 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
      901 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      961 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1021 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1081 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     1141 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1201 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1261 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1321 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1381 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
     1441 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     1501 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgctct
     1561 tcaggtctct aatggtttct aaaggagaag aactttttac tggtgttgta ccaatcttag
     1621 ttgaattaga tggagatgtc aatggtcaca aattttctgt ttctggagaa ggggaaggag
     1681 atgcaacata tggaaaactt actttgaaat tcatttgtac tactgggaaa ttacctgtac
     1741 catggcctac attagttaca actttatcat ggggtgtaca atgttttgca agatatcctg
     1801 atcatatgaa acaacatgat ttttttaaaa gtgctatgcc agaaggttat gttcaagaac
     1861 gtactatatt ttttaaagat gacggaaatt acaaaacgcg tgctgaagtt aaatttgaag
     1921 gagatacttt agttaatcga attgaactta aaggaattga ttttaaagaa gatggaaaca
     1981 ttttaggtca taaacttgag tataattatt tttctgataa tgtatatata acagcagata
     2041 aacaaaagaa tggcataaaa gctaatttta agattagaca taatattgaa gatggtggtg
     2101 ttcaattagc tgaccattat caacaaaata cccctattgg agatggtccg gtattacttc
     2161 ctgataatca ttatctaagt acacaatcaa aattatccaa agatccaaac gaaaaacgag
     2221 atcatatggt gttattagaa tttgtaactg ctgctggtat tacattagga atggatgaat
     2281 tgtacaaata agctttgaga cctgaagagc ccctgcagtc cggcaaaaaa gggcaaggtg
     2341 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
     2401 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2461 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2521 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
     2581 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2641 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2701 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2761 tctcatagct cacgctgtag gtatctcagt tcggtg
//
